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GFP-labeled and non-labeled cells from X blastoderm were large and round with the same characters of morphology. The GFP protein was not expressed in CEF cell .Our results suggested that the CVH promoter regions have specific activity in stage X blastoderm.

实验结果显示：在胚盘细胞转染后12 h可观测到绿色荧光表达，转染后24 h荧光细胞增多，细胞形态均呈圆形、体积较大；在鸡胚成纤维细胞未检测到GFP表达。

A small portion of BrdU and NeuN double labeled cells could be detected in the cortex at the early stage of development, and in the striatum and CA of the hippocampus in all groups. The majority of BrdU labeled cells were neuroglia, and the number of neuroglia cells decreased dramatically with brain maturation. Neurogenesis is the major cytogenesis in the dentate gyrus.

皮层在发育早期以及纹状体和海马CA区在发育各期检测到极少数为新生神经元细胞，多数分化为胶质细胞；海马齿状回以神经元细胞再生为主；胶质细胞的再生随脑组织发育的成熟而逐渐减少。

Some SPR-LI neurons were also positive for TH. After administration of vasopressin, SPR-LI structures became denser, especially at levels of pyramidal decussation and area postrema . The dendrites of motor dorsal nucleus of X in the dorsal part of MVZ appeared thin and straight in morphology instead of curl and thick outlooks. These results implicate that some SPR-LI neurons might receive or regulate the cardiovascular stress information induced by vasopressin.③In PHA-L/FOS/TH triple-labeled experiments, seven kinds of immunohistochemical structures were found in MVZ. They were FOS-LI neurons, TH-LI neurons, PHA-L positive fibers, PHA-L positive fibers terminated on FOS-LI or TH-LI neurons, FOS/TH doublelabeled neurons, PHA-L positive fibers terminated on FOS/TH double-labeled neurons.

在锥体交叉和最后区水平，SPR免疫反应阳性结构较正常组明显增强；背内侧部迷走神经运动背核的SPR纤维也由弯曲的粗纤维变为细的直纤维，30％的SPR阳性神经元为SPR/FOS双标记神经元，提示这些神经元参与感受心血管伤害性刺激信息，继而参与心血管活动信息的中枢传导或整合；③三重标记研究结果显示，在MVZ可见到七种免疫反应阳性结构，即PHA-L，FOS，TH，FOS/TH，PHA-L/FOS，PHA-L/TH，PHA-L/FOS/TH。

The new method combined the use of two DNA probes whose sequences comprises the whole complementary strand to the target DNA, in which one probe was labeled with an EDTA-〓 complex on the 5'-terminus and the other, labeled with a bidentate β-diketone on the 3'-terminus.

该法测定目标DNA的最低检测下限为6 pM，比现有其它均相DNA杂交分析法低2-3个数量级。该法具有简单、快速和高灵敏度等优点，通过使用各种的标记DNA探针，预计该法可广泛应用于各种实际DNA样品的分析。

Rhamnosus P15 to the skin and enteron mucus of Flounder by H3-TdR labeled and FITC labeled methods, and the effect of L.

本研究从牙鲆肠道分离益生菌并对其进行鉴定，研究了乳酸菌的粘附和定植，并探讨了益生菌在生产中的应用效果。

Methods mRNA of placenta from normal pregnancy and PIH were extracted and labeled with flourescent Cy3 and Cy5 in reverse transcription,respectively.The Biodoor chip was hybridized with labeled cDNA.

方法从正常妊娠胎盘和PIH胎盘中抽提mRNA，反转录后，分别用Cy3、Cy5荧光标记，获得两组胎盘来源的cDNA探针。cDNA探针与含8 190条人cDNA点样的BioDoor基因芯片杂交，结果由激光共聚焦扫描仪扫描，并用软件进行统计分析。

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗；(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力；(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性；(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡；(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型，应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况，明确其靶向性；(6)将荷瘤裸鼠分3组进行放射免疫治疗，分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

The one located between the two binary isomorphous phases,Ag_3Sn and Ag_3Sb,is labeled as ε and the other one located between the two binary isomorphous phases,Ag_4Sn and Ag_4Sb,is labeled as ξ.The c phase is a very stable phase and is in eq...

相为一很稳定的相，分别与ξ、Sb、SbSn、Sb_2Sn_3及液态 Sn 相平衡共存，Ag 在 Sb、SbSn 相的溶解度很低，且在此系统有一个计量系数化合物 Sb_2Sn_3。

In every group, rabbits were subdivided into experimental and control subgroups. 2 Rabbits were bullets injected followed by continuous injected with 13C labeled leucine, glucose, and lactic acid; 3 Blood were drewed before and 150, 160, 170, and 180 min after the initiation of isotopes injection for material analysis; 4 Exhale gas were collected every 5 min in the first 30 min followed by every 30 min there after for material analysis; 5 After centrifuge in low temp the supernatant of the blood samples were collected and went through axon and anon exchange column treatment; 6 Treated blood sample was used for 13C labeled leucine, glucose lactic acid examination through mass spectrograph; 7 The exhale gas was collected for 13CO2 exam through gas-phase mass spectrograph; 8 CO2 total production rate (V13CO2), body various substances production, oxidation speed, and substances metabolic percentage all can be calculated through equations provided by references below; 9 Data was processed through student t test.

分亮氨酸、葡萄糖和乳酸三组各取健康新西兰兔16只，每组再分为高代谢脓毒症组和对照组两部分，建模方法同第一部分；2）三组分别先静脉弹丸式推注再持续灌注13C标记的亮氨酸、葡萄糖和乳酸；3）灌注前抽取动脉血作为背景值，灌注达150，160，170和180min分别抽取动脉血2ml用于质谱分析；4）实验开始后前30min中每5min收集呼出气一次，随后每30min收集呼出气一次，用于气相质谱分析；5）血样经过低温离心取上清液，过阳离子及阴离子交换树脂，再经衍生化处理；6）处理过的血样进入气相色谱－质谱仪，测量其13C标记的亮氨酸、葡萄糖和乳酸丰度；7）实验兔的呼出气通过13CO2气相质谱分析仪测定其中的13CO2丰度（E13CO2）；8）利用文献提供的公式算出CO2总生成率（V13CO2）以及机体各物质的通量、氧化速率以及物质代谢百分比等；9）数据分析处理采用t检验。

Polymarase Chain Reaction may be the better method of detecting M.tuberculosis than other methods and lead us to carry out a series of research on this problem. This thesis is composed of five parts:(1)systematic evaluation for the first time of the ability of five defferent PCR methods to detect M. tuberculosis;(2) using the endonuclease and southern blot by a radioactive and a nonradioactive labeled probe, to confirm the specificity of the PCR products and to analyse the sensitivity of detection by southern blot method using digoxigenin-labeled probe;(3) evaluation of acid-fast staining, bacterial culture and PCR for the detection of M. tuberculosis in sputum, pleural and peritoneal effusion samples;(4) The feasibility of PCR for detection of M tuberculosis on formalin-fixed paraffin embedded tissues;(5) 26 cases of sarcoidosis were retrospectively studiedwith PCR for M. tuberculosis and nontuberculosis Mycobacterium and the problems on histopatholoical diagnosis of sarcoidosis are discussed.

论文共分为五个部分，(1)首次系统地比较了五种不同引物序列的PCR检测结核杆菌方法的特异性和敏感性等情况；(2)利用限制性内切酶酶切分析和同位素及非同位素标记DNA探针进行Southern杂交的办法，分析PCR产物的序列与原设计是否相符及杂交检测PCR产物对提高检测结核杆菌敏感性的情况；(3)并对PCR用于快速检测临床标本中的结核杆菌进行了初步评价；(4)探讨了PCR用于检测石蜡包埋组织中结核杆菌的作用；(5)并利用PCR检测结核杆菌和非结核分枝杆菌的方法对26例原病理诊断为结节病的病例进行了PCR检测及组织病理学诊断的探讨。

Methods: Five patients with parkinsonism or dystonia were assigned to general anesthesia using an modified endotracheal tube.

本实验依照人体实验之相关规定进行，五位患有帕金森氏症或肌张力异常的病人接受神经立体定位手术。

If you can benefit from this book, it is our honour.

如果您能从本书获益，这将是我们的荣幸。