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In fetal, otocyst cells proliferated and differentiated and showed several morphotypes, which labeled positive to F-actin and cytokeratin. A subset of cells were labeled by calretinin and BrdU.

4100ng/ml和200ng/ml bFGF二组中calretinin阳性着染的细胞数量分别占所观察的细胞总数的7％和7.6％，1ng/ml和10ng/ml bFGF组中，calretinin阳性着染的细胞数量分别为0.5％和1.6％。

To the problem that large-scale labeled samples isn't easy to acquire in the course of SVM training, the active learning strategy is used in the SVM training and an incremental training algorithm of active SVM based on the uncertainty based sampling of distance ratio is proposed in the paper. The experimental results show that the active SVM learning strategy can considerably reduce the labeled samples and costs compared to the passive learning method, and at the same time it can ensure the accurate classification performance is kept as the passive SVM and also expedite the SVM training.

针对 SVM训练学习过程中难以获得大量带有类标注样本的问题，本文将主动学习策略应用于SVM增量训练中，提出了一种基于距离比值不确定性抽样的主动SVM增量训练算法，实验结果表明在保证不影响分类精度的情况下，应用主动学习策略的SVM选择的标记样本数量大大低于随机选择的标记样本数量，大大降低了标记的工作量或代价，而且训练速度同样有所提高。

Then the labeled Hela cells were implanted into the subcutaneous tissue of nude mice and the growth of the implanted tumor was observed and growth curve of the labeled Hela cells was analyzed with the whole-body optical imaging system and the slide caliper.

BALB/cA-nu裸鼠皮下接种1×106个发光细胞使其成瘤，利用活体荧光成像系统和游标卡尺观察肿瘤的生长情况。

Gold colloids were labeled with Raman reporter mercapto molecules such as 4-mercaptobenzoic acid and thiophenol to form Fourier transform surface-enhanced Raman scattering reporter-labeled gold colloids.

以对巯基苯甲酸、苯硫酚为标记分子，与金纳米粒子生成具有SERS信号的标记金溶胶。

Proteins associated with the membrane and the cytosolic portions of the proteome under different copper ion concentrations were differentially labeled with an acid cleavable isotope-coded affinity tag reagent, and the labeled peptides were separated by two-dimensional chromatography and characterized by electrospray ionization-tandem mass spectrometry.

我们分别将在不同铜离子浓度下的细胞质以及细胞内膜中所有蛋白质标识上酸裂性同位素亲和性标识(acid cleavable isotope-coded affinity tag， cICAT)试剂，用二次元管柱层析将所有标识并酵素水解过的片段分离，并且用电洒式串连质谱(electrospray ionization-tandem mass spectrometry， ESI-MS/MS)以及基因体定序资料库决定蛋白质序列。

Therefore, in the present study, a retrograde and anterograde experiments were made in the rat to address this issue. After injection of cholera toxin B subunit, a retrograde tracer, into Barrington's nucleus or D-region, some retrogradely labeled neurons were found in lamina I, sacral parasympathetic nucleus and dorsal commissural nucleus of the lumbosacral spinal cord segments. Injection of biotinylated dextran amine into lamina I, sacral parasympathetic nucleus and dorsal commissural nucleus, respectively, a large number of BDA-labeled axon terminals were seen in Barrington's nucleus and D-region.

发现，将逆行示踪剂霍乱毒素 B 亚单位（ Cholera toxin B subunit； CTb ）注射到 Barrington's 核或 D 区后，在大鼠腰骶髓 I 层、骶髓副交感核和后连合核内分布有一定数量的 CTb 逆标神经元；将顺行示踪剂结合生物素的葡聚糖胺(Biotinylated dextran amine； BDA)分别注射到腰骶髓 I 层、骶髓副交感核和后连合核内后， Barrington's 核和 D 区内出现大量顺行标记的轴突终末。

All of them were labeled with [32P] orthophosphate (2.03×10^5 Bq/mL) for 2 h and then stimulated with CCK8 l0^(-7 mol/L and DMEM respectively for different time. Reactions were terminated by freezing neurons in liquid nitrogen. The 32P-labeled lysates isoelectrically focused on IPG Drystrip pH 4~7 linear gels and subsequently separated by second-dimensional SDS-PAGE.

培养乳小鼠神经元，随机分为CCK8刺激组和对照组，32P-NaH2PO4标记后分别给予CCK810^(-7 mol/L和等量无磷培养基，作用不同时间后液氮终止反应，经双向电泳、放射自显影，获得磷酸化蛋白质的电泳图谱。

Methods: the spiral modiolar artery in guinea pig cochlea was exposed by microsurgery, then the fluorogold or horseradish peroxidase was given around the sma for retrograde tracing. the bilateral superior cervical ganglia and stellate ganglia were observed to find retrogradely labeled neurons. the neurotransmitters of these sympathetic fibers around sma were ascertained by using retrograde tracing combined with immunohistochemical double labeled technique.

在正常豚鼠单侧耳蜗螺旋蜗轴动脉局部滴加逆行追踪剂辣根过氧化物酶或荧光金，观察双侧交感颈上神经节、星状神经节内hrp或fg逆标神经元的分布；采用逆标与免疫组化相结合的双重标记方法观察追踪阳性神经元是否表达酪氨酸羟化酶、神经肽y、钙基因相关肽或p物质。

It was found that only few log-phase trophozoites were labeled by means of C6-NBD-ceramide, however, most of the encysting trophozoites were labeled in perinuclear region.

结果 极少数蓝氏贾第鞭毛虫滋养体被C6-NBD神经酰胺标记；多数处于成囊6、12、18h时段的虫体均可见被特异标记成亮绿色的核周围区域。golgin245和Rab1在滋养体及成囊诱导6、12、24h的虫体中的表达水平无显著变化。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上，活细胞单层生长，胞浆较丰富，细胞核大小一致，有核仁×400 图2 SHEE培养细胞细胞核椭圆形，核仁较大，胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状，有伪足贴壁，表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁，呈星状或多角形，有丰富伪足和胞浆突，核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞，胞核和胞浆PI染色呈红色或淡红色，蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞，染色质凝集呈块状，右上角有一固缩细胞核TdT标记×400

On closer examination, though, this is not a vote for multilateralism but just the opposite.

仔细审视后我们发现，这并非是对多边主义投出的赞成票，而是恰好相反。

Uncovering their weak spots, so I can defeat them.

揭露出他们的弱点，这样我就可以打败他们了。