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Study on the Crystallization Process of Labeled GL-7-ACA Acylase CA130 Complex 7β-bromoacetyl amino cephalosporanic acid (BA-7-ACA), an analog of glutaryl-7-amino cephalosporanic acid (GL-7-ACA), can inhibit and specifically alkylate GL-7-ACA acylase (CA130) from Pseudomonas sp. 130, forming a carbon-carbon bond between BA-7-ACA and the C-2 on indole ring of Trp-β4 residue of CA130.Here we reported that BA-7-ACA labeled CA130 (BA-C130) could self-catalyze the hydrolysis of BA-7-ACA during crystallization process. The hydrolysis was confirmed to be a reaction analogous to the one of GL-7-ACA by comparative MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) spectrometry analysis.

二、GL-7-AcA酰化酶CA130标记复合物的结晶过程研究溴乙酰氨基头孢烷酸（7β-bromoacetyl amino cephalosporanic acid，BA-7-ACA）作为戊二酰-7-氨基头孢烷酸（GL-7-ACA）的类似物，不仅能够抑制GL-7-ACA酰化酶CA130的活力，而且能通过在BA-7-ACA和CA130的β亚基第四位色氨酸吲哚环二位碳原子之间形成碳-碳共价键而将CA130特异的烷基化。

Gonorrhoeae was amplified with the one set of primer for the 16S rRNA gene. The reverse primers were labeled with biotin. The products were identified by hybridization with its specific oligonucleotide probes labeled with amidogen in a nylon membrane.

选择NG 16S rRNA基因设计一对PCR引物，生物素标记下游引物扩增NG DNA，然后与固定在尼龙膜上的特异性寡核苷核探针杂交。

With the method of HRP retrograde tracing, some labeled cells were observed in the LA and the basal amygdaloid nucleus after horseradish peroxidate injection into A-Ⅰ. Most of these labeled cells were separately distributed in these nucleuses.

六HRP逆行追踪实验表明，在AⅠ区电泳HRP可在同侧LA和杏仁基底核内观察到逆行标记神经元，从而证实在大鼠上杏仁体向听区皮层的直接神经投射的存在。

All the probes were pointed on glass slides by GMS 417 Arrayer. Amplified plasmids wer.e labeled by PCR fluorescence(CY5)-primer and CY5-dUTP. The labeled plasmids were applied to the DNA chip. The hybridization was performed at scalar temperatures (43 C, 45 C, 47 C, 49 C, 51C) for one hour. After hybridization the chips were scanned by GMS418 Array Scanner.

采用PCR引物直接标记法和掺入法对HPv标准品进行扩增和荧光标记，将标记好的标准品与芯片在梯度温度下(43℃，45℃，47℃，49℃，51℃)杂交1 小时，用GMs 418 Array Sc~er扫描仪对杂交后的芯片进行扫描检测分析，用 sca'Array软件对各信号点进行信号强度采集。

To be protected hereunder, CONFIDENTIAL INFORMATION must be disclosed in written or graphic form conspicuously labeled with the name of the DISCLOSER as CONFIDENTIAL INFORMATION, or disclosed aurally and be documented in detail, labeled as above, and submitted by DISCLOSER in written or graphic form to RECIPIENT within twenty (20) business days thereafter.

在此之下被保护，机要信息如上所述必须被透露以书面或图表形式显眼地被标记以DISCLOSER 的名字作为机要信息，或听觉上被透露和详细被提供，被标记，和由DISCLOSER 递交以尔后书面或图表形式给接收者在二十(20)营业日内。

The cells labeled with PKH26 were seeded on the bio-derived bone to construct tissue engineered bone in vitro. Then the compound of cells and material were observed under fluorescence microscope. The compound of labeled cells and material were implanted into the rabbit thigh muscle, and the transformation of the labeled cells was observed by fluorescence microscope 14 and 28 days later.

将PKH26 标记的BMSCs 接种于生物衍生骨材料，48 h 后荧光显微镜观察细胞与材料的复合情况；将细胞-材料复合物植入成年新西兰大白兔双后肢肌袋，14、28 d 后荧光显微镜观察标记细胞的转归。

Culture and laboratory tests, including fluorescence-labeled antibody staining, PCR of virulence factor genes and Ascoli precipitin test on tissue specimens, identified the organism as Bacillus anthracis.

组织检体进行细菌培养及其他实验室检查，包括FA染色(fluorescence-labeled antibody staining)、毒力因子基因PCR及Ascoli precipitin test，证实病原为炭疽杆菌。

MAIN OUTCOME MEASUREES: Overlapping of TMR retrogradely labeled neurons,NOS positive labeled neurons and transganglionic tracing labeled terminals,and their relationship in INV.

主要观察指标：观察逆行TMR标记细胞、NOS阳性细胞以及跨神经节BDA标记的传入终末在INV内的重叠分布和三者的相互关系。

BMSCs were isolated, depurated, cultivanted, and identified,then incubated with the concentration of 25μg Fe per milliliter at 37℃in 5% CO2. The labeled cells were stained by Prussian blue/trypan blue,and observed under fluorescent microscope.2. The labeled cells of different density (1×104/ml,5×104/ml,1×105/ml,5×105/ml,1×106 /ml,5×106/ml)were imaged by MRI with T1WI, T2WI and T2*WI sequences;and the same density (5×104/ml,1×105/ml)labeled cells were imaged by T2*WI sequences at different time.Then the signal intensities were measured and statistically analyzed.3. The model of rabbit renal ischemia-reperfusion injury was set up and treated. Then BMSCs(5×105)were injected into 16 recipient rabbits(1abeled cells in 10,unlabeled cells in 6)from ear vein.MR images of kidneys were obtained respectively at the time points of 0,1,3,5, 8 days after transplantation and before transplantation. MR imaging findings were analyzed,which were correlated with histological findings.

实验方法1分离、纯化、培养、鉴定兔BMSCs并以SPIO以25μg Fe/ml培养液浓度标记，对标记后不同时间的细胞行普鲁士蓝染色和台盼蓝拒染后显微镜观察。2将不同细胞浓度标记细胞管(1×104/ml、5×104/ml、1×105/ml、5×105/ml、1×106/ml、5×106/ml)，以不同扫描序列T1WI，T2WI，T2*WI(GRE进行MR成像，再选择相同细胞浓度组(5×104/ml、1×105/ml)进行不同时相MR成像，并测量信号强度，进行统计学分析。3缺血再灌注肾损伤模型建立和处理，然后将标记和未标记细胞(5×105个)经耳缘静脉移植入家兔体内(共16只：注入标记细胞者10只，注入未标记细胞者6只)，两组均于注射前、注射后第0、1、3、5、8天应用MRI对移植细胞进行活体示踪并与肾脏组织切片对照，然后对收集的信号强度进行统计学分析。

Western blots were performed to investigate the cross-reactivity of various labeled antibodies with serum proteins from other fish species. Results Immunoglobulins of five fish species were purified, including bighead carp, carp, argus snakehead fish, ricefield eel, sea bass. The titers of five rabbit anti-sera obtained up to 1:32 examined by agarose double immunodiffusion. Five kinds of purified rabbit anti-fish immunoglobulins antibodies were labeled with HRP and their activities were up to 1:10000 examined by EUSA. Four kinds of rabbit anti-fish immunoglobulins antibodies labeled with HRP could react with serum proteins from other fish species.

结果 纯化了鳙、鲤、乌鳢、黄鳝、鲈五种鱼血清免疫球蛋白，免疫双扩散法测定兔抗这五种鱼免疫球蛋白的抗血清效价均达到1：32，并对纯化的兔抗鳙、鲤、乌鳢、黄鳝、鲈五种鱼类免疫球蛋白的抗体进行了HRP标记，ELISA测定标记抗体的效价达到1：10000左右，Western blots显示标记抗体与部分其他鱼类免疫球蛋白之间存在不同程度的交叉反应。

It goes back to what I told you...

现在回到我告诉过你的。。。

With a supporter in Mr Charest and an admirer in Mr Dumont, Mr Harper may be encouraged to call an election himself.

由于在沙雷那边有个支持者，杜蒙那边有个崇拜者，鼓励哈珀为自己举行一次选举。