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The results of its intrinsic fluorescence spectroscopy and fluorescence phase diagram showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed a partially folded intermediate of Bacillus amyloliquefaciens a-amylase during its unfolding procedure, which followed a three-state model; the result of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed some stable hydrophobic regions, which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid, in the partially folded intermediate of Bacillus amyloliquefaciens a-amylase; and the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed the distribution of Trp residues in Bacillus amyloliquefaciens a-amylase in different denaturation solution, with the maximum number (8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciens a-amylase molecule could be quenched by potassium iodide; and the results of their protein electrophoresis and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciens a-amylase formed during the whole unfolding procedure of Bacillus amyloliquefaciens a-amylase induced by guanidine hydrochloride.

内源荧光光谱和荧光相图结果表明，当变性液中盐酸胍浓度约为1.0 mol/L时，芽孢杆菌a-淀粉酶的去折叠过程中出现一个部分折叠中间体，其去折叠过程符合&三态模型&；荧光探针结果表明，在溶液中盐酸胍浓度约为1.0 mol/L时，中间态芽孢杆菌a-淀粉酶分子中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；荧光猝灭研究给出了不同程度变性的淀粉液化芽孢杆菌a-淀粉酶中的Trp的分布情况，结果表明中间态芽孢杆菌a-淀粉酶分子中能够被碘化钾猝灭的位于分子表面的色氨酸残基数目达到最大的8个；蛋白电泳和体积排阻色谱结果表明，在盐酸胍诱导的芽孢杆菌a-淀粉酶分子的整个去折叠过程中，不会以共价键或非共价键形式形成芽孢杆菌a-淀粉酶分子之间的集聚体或集聚体沉淀。

The upper shell and the lower shell are provided with an operating window for rotating the intermediate shell and a driven portion, which is engaged with a rack bar passing outside the operating window for rotating the intermediate shell up to a predetermined position, is provided on an external peripheral face of the intermediate shell.

上壳体和下壳体设置有用于旋转中间壳体的操作窗口，与通过操作窗口外侧的齿条配合用于将中间壳体旋转到预定位置的从动部分设置在中间壳体的外周边表面上。

Hongze Lake is located in the middle and lower reaches of the Huai River, and placed at climate intermediate belt of north and south, middle latitude intermediate belt, sea and land intermediate belt.

洪泽湖形成于公元16世纪，距今已有400余年历史，位于江苏省西北部淮安、宿迁市境内，是我国五大淡水湖之一，也是淮河流域最大的平原水库。

At first,the intermediate of sodium 3-chloro-2-hydroxy propanesulfate was synthesized from epichlorohydrin and sodium bisulfite;And then,using NaOH as a catalyst,the title compound has been synthesized from intermediate and allyl alcohol to reach the yield of about 70%.

先由环氧氯丙烷和亚硫酸氢钠合成中间体3-氯-2-羟基丙磺酸钠，然后中间体再与丙烯醇在氢氧化钠催化下生成目标产物2-羟基-3-烯丙氧基丙磺酸钠。

Intermediate image of three-mirror anastigmat having two image surfaces was between secondary and tertiary mirror. In order to fold the optical path, four-mirror anastigmat was designed by adding the spherical reflective mirror at intermediate image.

带一次中间像面的离轴三反系统的中间像位于次镜和三镜之间，为了进一步折叠光路，在中间像面处加入球面反射场镜，从而成为离轴四反。

Based on the theory of Sequence Stratigraphy and the systematically analysis of strata which was closely focused on Lithostratigraphy, Biostratigraphy, Paleoecology Event Stratigraphy and Sequence Stratigraphy, four long-term base-level cycles can be recognized in the Mesozoic, each long-term base-level cycles can be further divided into intermediate-term to short-term base-level cycle. Eight intermediate-term base-level cycles are recognized in Mesozoic; Cenozoic can be divided into two structural base-level cycles, and can be further divided into seven long-term baselevel cycles.

应用层序地层学理论，在详细的岩石地层、生物地层、生态地层、事件地层和层序地层为核心的综合地层研究基础上，将中生界划分为四个长期基准面旋回，每个长期基准面旋回内部又可进一步识别出更次一级的旋回，侏罗系内部共可识别出8个次级旋回；根据钻井层序地层与地震地层划分结果，将北缘地区新生界划分为两个构造基准面旋回，其内部又可进一步划分为7个长期基准面旋回。

All compounds are synthesized starting from the acylation reaction of gallic acid with L-glutamine to form the basic scaffold, followed by the cyclic reaction in acetic anhydride and the key intermediate cyclic anhydride (compound 4) was obtained. The intermediate compound is condensed with different amino acids or primary amines, and produces the target compounds.

本研究以L－谷氨酸为基本骨架，与甲醚化保护的没食子酸缩合后，经过酰化、环合反应得到关键中间体环状酸酐（化合物4），再经胺解反应合成目标化合物，其化学结构经过红外光谱、电喷雾质谱和核磁共振谱（氢谱、碳谱、碳氢异核多键相关谱）确证。

It is found that in certain water ratio and consolidation pressure, the values of initial elastic modulus and principal deviator stress increase with intermediate principal stress increasing; in certain intermediate principal stress, the values of initial elastic modulus and principal deviator stress increase with water ratio falling.

试验结果表明：在一定的含水率和固结压力下，偏差应力和初始切线模量均随中主应力比b的增大而增大；在中主应力比b确定的情况下，偏差应力和强度最大值随着含水率的增大而减小。

And direction of factor flows between intermediate sectors are dependent on the quantities of difference in sectoral technical progress and on, elasticity of substitution between intermediate products in the production of the final-product, respectively.

要素流动的速度取决于部门间技术增长率的差异，流动方向取决于中间产品在最终产品生产中的替代弹性。

A novel carbonyl transposition led by anilines'nucleophilic addition to methyl 2-keto α-D-glucopyranoside was discovered. And the mechanism of the reaction concerned to an enol rearrangment was provided through tracing the change of 〓H-NMR and 〓C-NMR of a hemiacetal intermediate which was obtained in the reaction of o-phenylene diamine with 2-keto glycoside. Both the intermediate and the converting product were identified by 〓H-NMR,〓C-NMR,〓H-〓H COSY and HMQC.

一、发现了2-位氧化α-甲基D-葡萄糖苷的芳胺亲核加成引起羰基转位的新方法，利用核磁共振氢谱及碳谱对邻苯二胺与2-位氧化α-甲基D-葡萄糖苷加成生成的稳定中间体半缩酮化合物在氘代丙酮中的变化进行跟踪，结合对转化产物的氢氢相关谱和HMQC结构确证，提出了该反应中2-位羰基通过烯醇化互变而转至3-位的机理并得到了验证。

The statistical analysis of this large set of mutations has led us to propose a diagnostic strategy that should help with the molecular work-up of optic neuropathies.

这一大系列基因突变的统计学分析引导我们提出一个诊断策略，可以帮助我们对视神经病变进行分子学的诊断检查。

The results show that the pipe grouting changes the mechanical parameters of surrounding rocks to achieve the security of engineering objectives by reinforcing surrounding rocks and bolcking the water.

研究表明：小导管注浆一方面改变了围岩的力学参数，另一方面利用浆液的固结特性可以实现堵水和加固围岩的工程目标，使隧道在开挖过程中的安全得到保障。