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Detection the early stage apoptosis cells by flow cytornetry Incubating SCL-1 cells in 6-well culture medium with 1×10~6/well, after 12～48hculture with the concentration of imiquimod 0μg/mL and 150μg/mL, gathering cellsgrowing along the wall and making monocellular suspension, washing in PBS for 2times, and then add 5μL Annexin V/FITC and 10μL PI solutions, reaction for 15min avoiding light, detection the early stage apoptosis cells by flow cytometry.

流式细胞仪测定细胞早期凋亡 SCL-1细胞以1×10~6个／孔接种于6孔培养板中，使用含0μg／mL、150μg／mL咪喹莫特的培养液培养12～48h，收集贴壁生长的细胞制成单细胞悬液，PBS洗两次，分别加入5μL Annexin V／FITC和10μL浓度20μg／mL的PI溶液，混匀后室温避光孵育15min，流式细胞仪检测，结果用Cell Quest软件分析。

HFCL cells can induce monocytic differentiation in HL 60 cells and weaken the ability of metastasis and invasion of HL 60 cells.

HFCL细胞能诱导HL 60细胞部分向单核细胞分化，并使趋化因子受体cxcr4表达下调。

When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.

选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。

The frequencies of micronuclei and multinucleate cells in BEAS-2B cells were significantly increased by DU and these increments of micronucleated cells could be inhibited effectively by DMSO.

贫铀诱发微核细胞、多核细胞增多，DMSO对微核细胞增多有抑制作用，而对多核细胞增多无明显的抑制作用。

Countless neutrophilic leucocytes were seen in 53 cases or 94.6%, plasma cells in 39 cases or 69.6%, reticulum cells in 34 cases or 60.7%, atypical epithelial cell in 39 cases or 69.6%, and multinucleated giant cells in 31 cases or 55.4% out of 56 cases of active phase ulcerative colitis.

在56例活动性溃疡结肠炎中，无数的嗜中性白细胞在53例（94.6%）中可见，浆细胞在39例（69.6%）中可见，网状细胞在34例（60.7%）中可见，不规则上皮细胞在39例（69.6%）中可见，多核巨噬细胞在31例（55.4%）中可见。

The results showed that the cells had classic characteristics of osteoclasts: multinucleated giant cells, staining positively for TRAP in cells, forming bone absorptive lacunae on the bone slices.

结果表明，直接分离的细胞具有典型的破骨细胞的形态特点：属多核大细胞，TRAP染色阳性，能在骨片表面形成吸收陷窝。

We have previously showed that CD40 activation induced the cell fusion and formation of multinucleated giant RS like cells from B lymphoma Daudi cells, which makes it clear that the Daudi cells obtained the ability of invasion after CD40 triggering.

CD40分子激发后的恶性B淋巴母细胞Daudi发生自身融合现象，表明肿瘤细胞在CD40分子激发后获得了某种侵润能力。而恶性B淋巴细胞的特性之一就是肿瘤细胞的广泛转移和侵润。因之，我们推测淋巴细胞的CD40分子激发可能对其扩散和转移会产生影响。

Observe the influence of combination therapy with microwave ablationand peritumoral injection of immature dendritic cells on survival and tumor growth in mice.Observe clinical safety of combination therapy with MWA and adoptive immunotherapy of dendritic cells and eJector cells.Research the change of percentage of lymphocyte subsets and concentration of cytokine in peripheral blood after combination therapy.Initially observe the short-term result of combination therapy.

目的摸索微波消融治疗时有效灭活肿瘤细胞并释放肿瘤抗原的温控和能量条件；观察肝癌消融后局部注射未成熟树突状细胞对荷瘤小鼠生存期及肿瘤生长的影响；观察肝癌微波消融联合树突状细胞疫苗及效应细胞综合过继免疫治疗的临床安全性，研究微波联合细胞免疫治疗后近期外周血淋巴亚群比例及细胞因子浓度的变化，初步探讨细胞免疫治疗对肝癌微波消融后近期复发率的影响。

rhBMP-2 suppresses the proliferation of HFCL cells, but has no effect on the differentiation of HFCL cells to myelocytic or mononucleate cells.

rhBMP-2可抑制HFCL细胞的增殖，但对HFCL细胞向髓系或单核方向分化影响不大。

In small intestine,the villous epithelial cells,Paneth cells and parasympathetic ganglion cells in myenteric plexus show GnRHR immunoreactivity.

三段小肠绒毛上皮细胞、潘氏细胞、十二指肠腺上皮细胞以及小肠肌间神经丛副交感神经节细胞，均呈较强的GnRH受体免疫反应性。

The role of the environment is very important.

的作用，环境是非常重要的。

At that time, the High Court of England and Wales Court of Queen's Bench president of William Murray, Lord Mansfield in June 22, 1772 ruling:"No matter then there is the inconvenience, but there must be a decision, I can not say that this case under the law in England are permitted or authorized; are so black should be released."

当时的英格兰及威尔士高等法院王座法庭院长William Murray， Lord Mansfield于1772年6月22日宣判：「无论有那麽不便，但总要有个决定，我不能说这件案在英格兰法律之下是准许或认可；所以黑人是应该被释放。