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RESULTS: Perforin positive cells were seen in the whole intralobular areas, especially in inflammatory areas. The amount of positive cells was different, 71.9% in spotty necrosis areas, 31.0% in focal necrosis areas, 9.2% in portal areas and only 4.8% in non necrotic areas. The average amount of perforin cells in the liver lobules of 18 patients had no linear correlation with the ALT level or Knodell's histology activity index.

结果： Perforin阳性细胞在整个肝小叶内均可检见，有炎性细胞浸润区均有perforin阳性细胞分布，浸润阳性细胞数量因病变部位的不同而有较大差异，慢性丙肝点状坏死区阳性率最高达71.9%，其次是片状坏死区(31.0%)，门管区阳性率为9.2%，小叶内非坏死区仅为4.8%。

Failure of B cells to undergo immunoglobulin isotype switch in this case is a result of mutation on the CD40L gene in the activated T cells and B cells may have the ability to secrete IgG. This is the first case of HIGM with X-linked inheritance diagnosed at a molecular level in China.

患儿B细胞可能具有正常Ig分泌功能，Ig同种型转换障碍是由于其T细胞CD40L基因缺失，并在国内首次从分子水平上确诊一例性联高IgM综合征患儿。

The cytomorphological changes of K562 cells were observed in the electron microscope.Results RRTJ at the concentration of 1μL/mL expressed obviously inhibitory effect on the growth of K562 cells.The inhibitory effect at the concentration of 2μl/ml went up to its highest at 83.4%.RRTJ showed virulence to K562 cells.The karyon changed because of RRTJ attending.

结果 每mL培养液含1μL刺梨汁即对肿瘤细胞增殖有明显的抑制作用，每mL培养液含2μL刺梨汁抑制作用达到最大，为83.4%，再加大剂量抑制作用无明显升高，各种剂量的刺梨汁对细胞均无毒性，刺梨汁的加入使得细胞体积明显变小，细胞核形态发生改变，异染色质增多。

Results Nicotinamide at 1-5 mg/ml induced apoptosis of GLC-82 cells in a dose-dependent manner, and group 4 mg/ml and group 5 mg/ml showed increased apoptosis than other groups. Distinct morphologic changes of cell apoptosis such as karyopyknosis and conglomeration were observed; Cell migration became slower as the dosage of Nicotinamide increased;Cells produced an arrested at G1 phase, accompanied by a reduction of cells transiting through S phase.

结果 烟酰胺诱导细胞凋亡程度与用药剂量呈正相关，较高剂量的药物能引起GLC-82肺腺癌细胞呈现边界不清，体积缩小，核固缩，染色质凝集、边缘化，甚至细胞发生破碎，凋亡小体形成；随着烟酰胺浓度的增高GLC-82细胞向划痕区的愈合速度不断减慢；烟酰胺的浓度大于等于3 mg/ml 时，细胞周期主要表现为G1 期比例的上升伴随S期比例的下降。

Results When the cells were treated with Sodium Selenite at a concentration of 2.0mmol//L lasting 72h, karyopyknosis and chromatin condensation were observed under microscope; When the cells were treated with Sodium Selenite at a concentration of 4.0mmol/L lasting 72h, the cells in G0/G1 and S phase was significantly decreased, a typical apoptosis peak before G1 phase was shown.

结果 2.0mmol/L亚硒酸钠作用72h， HE切片可见核固缩、染色质边集等改变；4mmol/L亚硒酸钠作用72h， G0/G1期和S期细胞明显减少，在细胞周期G1前期可见典型的细胞凋亡峰。

The expression changes of relating-apoptosis gene proteins (bcl-2, bax) were detected by immunohistochemistry before and after treating with the Agaricus Blazei Murill extract to explore the possible mechanisms of inducing apoptosis for MGC-803 cells in vitro. Results: The Agaricus Blazei Murill extract significantly inhibited the proliferation of gastric cancer cells in vitro and the inhibitive effects were depended on the medicine concentration and treating times. After treating 24 hours on the gastric cancer cells with the morphologic changes of apoptosis with chromatin margination, karyopyknosis, karyorrhexis were found by the light.

结果：(1)通过细胞培养和MTT法，表明长白山姬松茸在体外对MGC-803细胞株有明显的抑制作用，加药组与对照组相比其生长抑制率有显著性差异(P＜0.01)，而且这种抑制作用呈现浓度和时间的依赖性；(2)通过集落形成率的测定结果表明，加药组与对照组相比其集落形成率和集落形成抑制率有明显差异(P＜0.01)，说明长白山姬松茸对MGC-803胃癌细胞株有明显抗增殖作用；(3)光镜观察结果表明，加药组可见细胞脱水浓缩伊红染色增强，胞体缩小，内含高度浓缩的胞核呈深蓝色等典型细胞凋亡形态；经AO／EB荧光染色观察结果表明，当终浓度为1.0mg／ml的姬松茸提取物作用于MGC-803胃癌细胞24h后，其凋亡率和破膜率与自然凋亡率与破膜率相比，均有显著性差异，其凋亡率86.3％(P＜0.001)，破膜率为41.6％(P＜0.01)，说明姬松茸确实有诱导MGC-803胃癌细胞凋亡的作用，同时也有细胞毒作用，但以诱导细胞凋亡为主；(4)免疫组化结果表明，用药前后凋亡相关基因的BCl-2、Bax蛋白均有显著性的改变(P＜0.001)。

After 4 days of serum deprivation, CPR-293 cells exhibited substantial nuclear condensation and fragmentation DNA laddering but HO-1/CPR-293 cells was not apparent. Thus HO-1 expression is sufficient to protect cells from serum deprivation-induced apoptosis. In the experiment, we used primary cultures of from HO-1 fibroblasts and their wild-type littermates fibroblasts CPR-293 cell and HO!

2生理加压诱导细胞凋亡我们用稳定的CPR细胞株和HO1和CPR在一起的细胞株实验，当浆液丢失4天后，CPR-293细胞表现出有实质的核浓缩和DNA梯状条带的形成，而HO1/CPR-293细胞则没有，显示HO1是充分保护细胞，避免在浆液丢失情况下而诱导的细胞凋亡。

The distribution density of oil cells, the distribution types of oil cells and mucilage cells and their localization in the mesophyll are of some taxonomic value at the specific level in the Lauraceae.

油细胞的分布密度、油细胞与粘液细胞是否共存、是否单独存在或无，以及其具体分布部位等方面在种间存在一定的差异，这对于属以下等级的区分具有一定意义。

In addition, SW480 and HCT116 cells were treated with deoxycholic acid for 15, 30, 45, 60, 90 and 120 minutes. It was found that the deoxycholic acid treated cells showed an elevation of ATP activity by 12.35 %, 20.08 %, 29.69 %, 33.56 %, 45.85 %, 54.32 %, respectively. While the HCT116 treated cells, showed an elevation of ATP activity by 3.69 %, 5.59 %, 8.52 %, 12.26 %, 16.52 %, 19.71%, respectively.From the experiments conducted using the cell lines described above, it was further confirmed that genes CYP7A1 (cytochrome P450, family 7, subfamily A, polypeptide 1), AKR1D1 (aldo-keto reductase family 1, member D1), ALDH1A1 (aldehyde dehydrogenase 1 family, member A1), BAAT (bile acid Coenzyme A: amino acid N-acyltransferase), ABCA1 (ATP-binding cassette, sub-family A, member 1), APOC3 (apolipoprotein C-III), LIPA, LIPE (lipase, hormone-sensitive), PTGER2 (prostaglandin E receptor 2) and PTGS2 (prostaglandin-endoperoxide synthase 2) were up-regulated.

此外，为了证实其脂肪代谢产物胆酸对细胞癌化的影响，亦使用SW480和HCT116细胞株，经由脱氧胆酸在15分钟、30分钟、45分钟、60分钟、90分钟和120分钟处理后，其细胞增生比率分别有12.35%，20.08%，29.69%，33.56%，45.85%和54.32%(p.05)以及3.69%，5.59%，8.52%，12.26%，16.52%和19.71%(p.05)上升情形，并从这些细胞株进一步证实CYP7A1(细胞色素P450-7A1)、AKR1D1(醇醛酮还原酶-1-D1)、ALDH1A1(醛脱氢酶-1-A1)、BAAT(胆酸辅酶A：氨基酸N-醯基转移酶)、ABCA1(三磷酸腺苷结合盒转运体A1)、APOC3、LIPA、LIPE(脂肪酶/激素敏感脂肪酶)、PTGER2(前列腺素E受体2)和PTGS2(前列腺素内过氧化物合成酶2)等基因表现皆有呈现向上调控的情形。

L To observe the expression rule of miRNA when the lipocyte stem cells differentiated into cartilage cells or bone cells in different micro-environment and stages by in vivo tests and in vitro study.

通过体内、外试验，观察不同环境、不同阶段家猪脂肪干细胞向软骨、成骨方向分化时miRNA表达的规律。（3）研究双层一体Collagen/TCP影响家猪脂肪干细胞 miRNA表达的变化，并验证与鉴定成软骨、成骨分化相关的miRNA。

Effective local search tactics reaches local searchers when they are ready to buy - with every way that it is possible for them to buy from you.

有效的地方搜索策略达到当地的搜索，当他们准备购买-以各种方式，这是他们有可能从您那里进行购买。

The first two are periodic sentences for their beginnings; and the fifth and the last are also periodic sentences each for the embedding of a parenthetic structure.

1960年代中后期，当战后女权主义和当时的许多其他抗议运动在西欧和北美发展成某种势力时，性别的概念演绎成补充和延伸生物性别差异的概念。