查询词典 induced bundle

To get more information of the function of human Era on cell cycle, the complete control inducible mammalian expression system was used to express human Era mutant and analyzed cell cycle of uninduced (add 1% ethanol) and induced (10μM Pon A) cell lines by flowcytometer, the primary results demonstrated human Era mutant could inhibit apoptosis induced by ethanol, and Era S36N may stimulate the expression of Bcl-2 as detected by Western Blot.

在构建稳定高表达蜕皮激素受体基因的细胞株3V3的基础上，转染突变型人Era的表达载体进行压力筛选，对所得到的单克隆细胞株进行诱导与未诱导条件下细胞周期的分析，结果表明未诱导加酒精的对照细胞可以引起细胞凋亡，而Era诱导表达后可以显著抑制凋亡细胞的数量，Western表明Era S36N可以诱导细胞Bcl-2表达，提示人Era可能与细胞凋亡相关。

A 1: Uninduced supernant of cell lysate by sonication; 2: Induced supernant of cell lysate by sonication; 3: Uninduced precipitate of cell lysate by sonication; 4: Induced inclusion body of cell lysate by sonication; 5: Purified ULBP4 protein; M: Low molecular marker proteins.

2.3 体外NKG2D结合实验和细胞因子分泌实验间接ELISA检测结果显示：原核表达复性后的ULBP4能与人NKG2D结合，而带His标签的无关蛋白则不能与之结合，说明复性后的ULBP4仍能维持正确的天然构像。

ANP and CX43 began to express at 2nd week after induction and increased gradually,about 60% of the resulting myogenic cells were positive at 4th week after induction ,they were negative for uninduced cells.hMSCs'surface antigen profiles obtained by Flow Cytometry were negative for CD31\CD34\CD45 before and after induction,but CD90 expressed higher after induction while was weak positive before induction(P.05). Apotosis index was correlated with the cultural time after induction,The apoptosis rate of induced hMSCs was remarkably higher than control group(P.01),and the variation between groups was notable(P.05),the cell cycle analysis showed that the percentages of G_0/G_1phases were reduced significantly after induction. The expresstion levels ofβ-MHC and CTNT mRNA were undetectable before induction,began to increase at 1st、4th week after induction,reached the peak at 6th week and decreased after that,the expression of Bcl-2 and Bax mRNA varied regularly after treated with 5-azacytidine. hMSCs'resting membrane potential、range and rate of depolarization were heightened gradually after being induced.

结果：hMSCs诱导前为纺锤形，诱导后第2天部分细胞即开始发生形变，呈球形或短棒状，1周后胞浆中颗粒增多，约20～30%细胞边缘呈毛刷样变化；hMSCs表面抗原CD31、CD34、CD45在诱导前后差异无统计学意义，CD90未诱导时表达呈弱阳性，诱导后明显增高(P.05)；ANP和CX43在诱导前无表达，诱导后第2周开始表达且表达随时间逐渐增强，但CX43在诱导后第5周表达量开始降低。hMSCs诱导后凋亡指数随诱导后培养时间增加，低浓度诱导组低于标准浓度诱导组，组间差异有统计学意义(P.05)，G_0/G_1期细胞比例诱导后较对照组显著减少(P.05)；β-MHC和CTNT基因分别在诱导后第1周和第4周时表达开始增强，在第6周时均达到高峰，第8周时表达开始衰减，Bcl-2、Bax基因表达呈时间依赖性变化，hMSCs经诱导后随心肌样细胞特征的表达膜静息电位、去极化幅值和去极化速率逐渐增高。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Instant electric field-induced domain switching, crack initiation and propagation in a poled BaTiO3 single crystal, as well as sustained field -induced indentation crack propagation and 90°domain switching in an unpoled BaTiO3 single crystal have been investigated in situ using optical microscopy.

金相显微镜原位观察了面内极化的BaTiO3单晶在瞬时电场作用下的畴变和裂纹形核、扩展的关系以及未极化BaTiO3单晶在恒电场下Vickers压痕裂纹扩展和电致畴变的关系。

The research which is the important part of the tunable erbium-doped fiber laser is composed of two main parts: 1. the gain part of the tunable erbium-doped fiber laser, which in fact is an erbium-doped fiber amplifier, its output characteristics are discussed both in theory and experiment, and the differences are also discussed between theory and experiment; 2. the saturated absorber part of the tunable erbium-doped fiber laser, also the part to narrow the line width of the fiber laser. The line-width narrowing mechanism in fiber laser induced by unpumped Er-doped fiber is presented from the aspect of coupling wave equation, and the main factor is considered as the standing wave induced by interference. The line-width is narrower when the standing wave is stronger, and the narrowing effect disappears with the disappearance of the standing wave. In the experiment, when adding a Faraday mirror in the laser cavity to remove the standing wave , the narrowing effect disappears. So the theory is proved by the result of the experiment.

本课题的研究内容是集成光学声光可调谐掺铒光纤激光器的重要组成部分，主要分两部分：1、声光可调谐掺铒光纤激光器中增益部分的研究，相当于对掺铒光纤放大器的研究，理论上对掺铒光纤放大器的输出特性进行分析，实验上实现了掺铒光纤放大器系统，并将实验数据与理论计算数据进行对比，分析了误差产生原因；2、声光可调谐掺铒光纤激光器中饱和吸收体部分的研究，即压窄光纤激光器输出线宽的研究，从耦合波方程出发，理论上解释了未泵浦掺铒光纤压窄光纤激光器线宽的原理，分析了影响该作用的主要因素是未泵浦掺铒光纤中相对传输的光干涉形成的驻波：驻波越强，压窄效果越好，相反当没有驻波时，没有压窄效果，在实验上通过在激光器腔内加入法拉第旋转镜使驻波消失，同时压窄效果消失，从而验证了理论分析的正确性。

MeJA treatment induced vanillic acid and caffeic acid productions; whereas treatment of MeSA and the damage caused by third instar larvae of the maize stalk borer induced the production of only caffeic acid and p－Hydroxybenzoic acid respectively.

三种浓度的MeJA处理均使根际土壤细菌群落相似性指数显著降低，6μL/L的MeJA处理使细菌群落的多样性指数显著降低。

The outbreak was induced by eating fresh or half-fresh pomacea canaliculata,indicating that outbreak of verminosis induced by food can also happen in the northland.

本次疫情属于生吃半生吃福寿螺螺肉而发生的广州管圆线虫病暴发流行，提示食源性寄生虫病在北方也存在暴发可能。

Methods Both in vitro and in vivo models of ventilator-induced lung injury were used. Alveolar epithelial cells were stretched in vitro to mimic the lung injury in VILI. Rats were ventilated at large tidal volume to produce ventilator-induced lung injury in vivo. A total of 23 inflammatory cytokines were screened with micro gene array in stretched alveolar epithelial cells.

方法通过呼吸机诱导肺损伤的体内及体外模型，将分离的肺泡上皮细胞于体外拉长，利用基因微阵列技术分析并筛选出上调的炎性细胞因子，之后给予大鼠呼吸机机械通气，留取血清标本，测量这些细胞因子在血清中是否上调。

Compare the lung injury extent and the important physiological parameters to determine the optimal condition for rabbit ventilator induced lung injury, Choosing another 24 new Zealand rabbit, randomly divided into 4 groups:(1)conventional ventilation+PEEP group;(2)ventilator induced lung injury group: employ the experimental condition determined by the first part experiment;(3)VILI+HO-1 inducer hemin group:pre-treated with HO-1 inducer, the ventilation method is the same with VILI group;(4)VILI+HO-1 inhibitor Zn-PP group(Zn-PP group):pre-treated with HO-1 inhibitor, the ventilation condition is the same with VILI group.

第二部分24只家兔随机分为4组：(1)常规潮气量+PEEP组；(2)呼吸机所致肺损伤组：使用实验第一部分确定的最适条件；(3)呼吸机所致肺损伤+HO-1诱导剂干预组：预先给予氯铁血红素，通气条件同VILI组；(4)呼吸机所致肺损伤+HO-1阻断剂干预组：预先给予锌原卟啉，通气条件同VILI组。

This matter overthrew all basises I learned from Sunday school .

这件事把我从周日学校学到的所有道理都颠覆了。

It's one of the cruel ironies of aging: if you want to remember your favorite restaurants, the less you should eat at them.

这是一个老化的残酷讽刺：如果你想记住你最喜欢的餐馆，你应该更少吃到它们。