查询词典 induced bundle

PTX at tenuates the ALI induced by LPS or sepsis in rat. The therapeutic effects of PTX include:improve oxygenation, at tenuate pulmonary edema formation, enhance animal survival in sepsis-induced ALI. The mechanisms may related with the effects of PTX inhibiting the chemotaxis, migration, activation of inflammatory leukocytes, diminishing the production of active oxygen, and inhibiting inflammatory cytokine release partly.

4PTX对大鼠内毒素性ALI及脓毒症性ALI均有一定的防治作用，主要表现在改善动脉血氧合，减轻肺水肿形成，提高脓毒症性ALI大鼠的存活率等方面，其机理可能与抑制白细胞趋化与激活，减少肺内白细胞渗出及活性氧产生，一定程度上抑制炎性细胞因子生成等有关。

NodD binds to and bends target promoters through anchoring two tandem and individual specific DNA sites. NodD functions as a tetramer, which has a V-shaped main body. Tetrameric NodD is to change its own conformation rather than its oligomeric forms in response to small signal molecules. The specific interaction between each NodD DNA-binding domain and each specific DNA site does not alter itself in spite of naringenin induction, and the induced conformational change is transferred from protein to DNA. Only the DNA conformation incited by induced NodD is competent for RNA polymerase to form the transcriptional open complex. It cannot be excluded that NodD may have protein-to-protein contacts with RNA polymerase, and that the NodD conformational change may also directly contribute to the transcriptional open complex formation. However, the NodD conformational change itself cannot serve as the determinant of the transcriptional molecular switch.

通过研究，我们提出了初步的NodD操纵子激活模型：第一，四聚体是NodD蛋白的功能单位，它通过铆钉两个串联的相对独立的DNA靶位点结合被诱导的启动子；第二，小分子配基的结合是改变NodD四聚体的构象而不是引发不同形式的寡聚体，在我们的模型中，NodD四聚体缩小其V形主体的弯折角，进而缩短其DNA结合功能域的间距；第三，小分子信号的诱导并没有改变NodD的DNA结合域和其DNA靶位点的相互作用，NodD的构象改变由蛋白质经其双铆钉位点传递给DNA；第四，只有诱导状态的NodD激发的DNA构象才能有效地使RNA聚合酶形成转录开放复合物；第五，不排除NodD与RNA聚合酶可能有直接的相互接触位点，不排除NodD构象的改变可能直接有利于RNA聚合酶形成转录开放复合物，但是我们认为NodD构象改变本身不是充当转录激活开关的决定因素。

Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.

第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml，分离单个核细胞体外诱导培养DC，并将实验组和对照组细胞各分为三组，A组为未处理组，B组为冻融组，C组为RNA转染组。A组DC在培养5d后，每孔加入TNF-α200U/ml；B组DC培养第5d后，每孔中加入癌细胞蛋白抗原，使DC/肿瘤细胞=1：1，继续培养4h后，每孔加入TNF-α200U/ml；C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC：RNA=1×105/ml： 5ug，按照invitrogen DMRIE-C试剂说明书进行操作)，转染结束后用完全培基取代转染剂，每孔加入TNF-α200U/ml。

Methods Quantitative immunoblot analysis was used to examine sIgE and tIgE level in 364 chronic eczema children. All subjects were tested for food-induced and airborne-induced allergens equally.

采用德国Mediwiss过敏原体外检测系统，应用免疫印迹定量法对364例慢性湿疹患儿进行血清特异性IgE和总IgE检测。

Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2～3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.

本文将对氧化还原状态变化敏感的绿色荧光蛋白roGFP1-R12，在酵母细胞中实现了多拷贝强表达；荧光扫描经强氧化剂H_2O_2和还原剂DTT以及环境中重金属NaAsO_2或Pb(NO_3)_2处理后的酵母细胞悬液，测定510 nm处的荧光发射强度结果显示，表达的绿色荧光蛋白对氧化还原水平敏感，且在510 nm处的荧光强度与一定的重金属浓度呈正相关，即roGFP1-R12在510nm处的荧光发射值随重金属浓度的增高而增强，从而说明重金属对细胞的毒性在一定程度上很可能是通过破坏细胞内的氧化还原平衡发生作用；同时通过该绿色荧光蛋白对胞内氧化还原状态变化的响应情况可以来实时检测环境中的重金属；遗传学的点突变频率及致死率实验数据表明，重金属能导致菌体的点突变频率和致死率升高，且活性氧的清除剂巯基脲能明显降低这种点突变和致死率，说明由重金属引发的这种点突变和致死效应在很大程度上是依赖于重金属对细胞诱导产生的氧化胁迫。

Calcium ionophore-induced tryptase release was inhibited 60% and 69% by 10 and 50 microM of DL, respectively, and anti-IgE-induced tryptase release was inhibited 33%, 47% and 66% for 1, 10 and 50 microM of DL.

研究发现浓度分别为1、10和50mM的DL分别使钙离子载体诱导的LTC4释放下降29%、35%和39%，分别使IgE诱导的类胰蛋白酶释放下降33%、47%和66%。

Effects of Simvastatin on the Transdifferentiation of Renal Tubular Epithelial Cells Induced with High Glucose UANG Xiao-xia,KE Chang-bin,ZHANG Qing-hong,ZHANG Jian-e*（Department of Nephrology;Department of Anesthesia,Taihe Hospital,Yunyang Medical College,Shiyan,Hubei 442000,China）Abstract:Objective To investigate the effects of simvastatin in renal tubular epithelial cells transdifferentiation induced with high glucose.

作者：黄晓霞，柯昌斌，张庆红，张建鄂*作者单位：郧阳医学院附属太和医院肾病内科；麻醉科，湖北十堰 442000 目的：观察辛伐他汀对高糖诱导的肾小管上皮细胞转分化的作用。

Two special TL phenomena have been found in Yanzhuang meteorite through the determination of the natural TL, annealed TL and induced TL by β radiation: l.when the sample of Yanzhuang meteorite is annealed at temperature up to 500℃, the TL peak induced by β rays moves to lower temperature with the increasing of irradiation dose; 2.when the annealed temperature is greater than 600℃, the TL peak temperature of the annealed sample is higher than that of the unannealed sample.

991年10月31日21点45分在广东省翁源县岩庄乡境内发生了陨石降落。从降落现场收集到3.skg的陨石样品，经初步鉴定为H6型、具有强冲击变质特性的珍稀普通球粒陨石。根据陨石命名规则定名为岩庄陨石。普通球粒陨石是目前研究太阳系早期所发生的化学与物理变化过程的最好的信息来源。

The HSS action showed a dose-effect manner and this action was proved uneffective with the larger dose of CCl〓.(2) Hepatic histological findings indicated that CCl〓-induced hepatic lesions could been alliviated by HSS.(3) CCl〓-induced reduction of hepatic mitochondrial succinic dehydrogenase activity was restored by the treatment of HSS.

相反，如果CCl〓所用剂量过大，HSS将无抗损伤作用；(2)HSS可使CCl〓损伤肝组织的程度减轻，用肝组织切片可充分证明这一现象；(3)HSS可使CCl〓损害肝细胞线粒体琥珀酸脱氢酶的活性恢复，用组织化学法可得到证实。

In this study, We used longer DD primers in combination with a two-step PCR protocol to overcome these two problems in term of modified methods of Martin and Pardee.K562 erythroleukemia cell differentiation induced by HEMIN or PMA for 36 hours was used as erythriod or megakaryocytic differentiation model in vitro.Total RNAs were extracted from induced and uninduced K562 cells and were applied to mRNA differential display analysis.

为解决此问题，我们参照Martin和Pardee的改进方案［3］，建立了一种使用长引物，进行二步PCR的改进DDRT-PCR方法，以氯高铁血红素和肉豆蔻佛波酯诱导K562细胞36小时作为体外红细胞和巨核细胞分化模型，取诱导前后的细胞总RNA进行mRNA差异显示分析。

This matter overthrew all basises I learned from Sunday school .

这件事把我从周日学校学到的所有道理都颠覆了。

It's one of the cruel ironies of aging: if you want to remember your favorite restaurants, the less you should eat at them.

这是一个老化的残酷讽刺：如果你想记住你最喜欢的餐馆，你应该更少吃到它们。