查询词典 hybridized

Today, the unregistered hybridized Alaskan husky is the preferred choice for dogsled racing.

今天，未有登记记录的杂交阿拉斯加爱斯基摩狗是狗拉雪橇比赛的首选品系。

Results 371 bp DNA fragment was amplified from 24 different species. The sensitivity could be improved to 10-12 g. No signal was observed when human DNA,funguses and viruses were used as templates. 22 blood samples and 4 cerebrospinal fluid samples, being positive with culture, were positive by using PCR. The gramnegative and grampositive probes hybridized to clinic samples and different species, as predicted by Gram stain characteristics.

结果 对24株不同标准菌株进行PCR扩增，均出现371 bp长度的DNA片段，敏感性试验可检测出10-12 g的细菌DNA，与人类基因组DNA、真菌及病毒无交叉反应；22例血培养阳性标本及4例脑脊液培养阳性标本均扩增出371 bp长度DNA条带，反相杂交法区分革兰阳性/阴性细菌与培养结果相符。

The yield and purity of each DNA sample was tested by UV-spectrophotometer, the integrality of these DNA samples were run electrophoresis on the agarose gel. Each DNA sample was subjected to PCR amplification and hybridization using One lambada rSSO HLA-A,-B and -DRB1 commercial kit, the fluorescent intensity for positive bead and negative bead hybridized with HLA-A,-B and -DRB1 PCR products were calculated and analyzed.

使用2ml深孔板和TECAN DNA全自动工作站，从288份骨髓捐献者样本中提取基因组DNA；提取的DNA样本用紫外分光光度仪测定其浓度和纯度，并采用琼脂糖凝胶电泳检测DNA的完整性；DNA样本用One lambda rSSO HLA-A、B和DRB1基因分型试剂盒进行PCR扩增、分子杂交和Luminex流式磁珠分析仪检测，统计分析每一DNA样本HLA-A、B和DRB1基因扩增产物经DNA探针分子杂交后的阳性磁珠和阴性磁珠的荧光信号强度。

During the first cross, 4 different white monokaryotic strains were hybridized with 14 kinds of yellow monokaryotic ones to obtain light-yellow strains, which were then used to fruiting experiment. In the second cross, five kinds of yellow monokaryotic strains, which were observed monokaryotic fruit body, and 12 types of white monokaryotic ones were crossed to acquire hybrid strains, which were also to the experiment of fruit body formation.

第一轮杂交，运用4个不同的白色单核菌株和14个不同黄色单核杂交，获得系列浅黄色杂合子菌株，用于出菇实验；第二轮杂交，运用5个能够获得单核子实体的不同黄色单核菌株与12个不同的白色单核菌株相互杂交获得杂合子菌株，用于出菇实验。

The long specific DNA probes were synthesized by polymerase chain reaction in the highly specific gene sequences of above 10 bacteria. The species-specific probes synthesized were inoculated on a nitrocellulose filter and hybridized with template DNA labeled with long-arm photosensitive biotin. The signal of hybridization reaction was observed to detect which bacteria infected. The sensitivity and specificity of the method were studied.

在这10种病原菌高度特异的基因序列内利用聚合酶链反应合成上述10种细菌特异的长链DNA探针，并将合成的特异探针固定在硝酸纤维素膜上，与用长臂光敏生物素标记后的模板DNA进行斑点杂交反应，通过观察杂交信号判断存在何种细菌感染，同时研究该方法的敏感性和特异性。2。

Brief probe hybridized with long target DNA, the hybridization position had no effects on the binding amount, but mid-position of hybridization would prolong the hybridization time, which was due to the steric hindrance on the surface.

结合位点不同对杂交量无明显影响，但由于晶体表面DNA空间结构的影响，结合位点位于靶序列中间时杂交时间明显延长。

RT-PCR was a sensitive and stable method. It would be useful for large-scale diagnosis.A length cDNA of CEVd had been cloned into plasmid vector. Biotinylated probes were prepared from these plasmid DNAs. CEVd were examined from the positive and netative materials by Dot-blotting hybridization and imprint-hybridization. The result was exposed to x-ray film. Singles were detected for positive materials spots probed with probe, indicating that under the reaction conditions the probe hybridized with the target viroid specifically.

核酸杂交法检测：利用已经克隆的美国柑桔裂皮类病毒cDNA序列，制备生物素标记的核苷酸探针，采用斑点杂交的方法和组织印迹的方法分别对阳性材料进行杂交检测，通过胶片曝光显示杂交信号，发现在两种方法的检测结果中阳性材料都有强的反应，而阴性材料无反应或较弱，该方法安全可靠、快速准确，适合大量样品的检测。

The genomic DNA of Thinopyron intermedium labelled with biotin-16-dUTP was hybridized with the chromosomal DNA of Wheat-Th. intermedium addition Line Z,(2n = 44), which had a good resistance to barley yellow dwarf vims.

以生物素（Biotin－16－dUTP）标记中间偃麦草基因组 DNA为探针，与抗黄矮病小麦－中间偃麦草染色体异附加系Z6进行原位杂交，鉴定出附加的1对中间偃麦草染色体。

The replicon of irp-2 gene was identified by PCR method in 64 of 67 strains (95.5%) hybridized with irp-2 probe.

鉴于在一部分肠侵袭性大肠杆菌中可检测到irp-2基因，因而检测了EIEC的侵袭性基因ipaB基因。

The protocol is summarized in Figure 18.16. A radioactive probe representing a gene (most often a labeled cDNA clone derived from the mRNA) is hybridized with thedenatured DNA of the polytene chromosomes in situ. Autoradiography identifies the position or positions of the corresponding genes by the superimposition of grains at a particular band or bands.

如图26.15所示为此过程草图：一个放射性探针代表一个基因（常是一个从mRNA中克隆得到的cDNA），它与原位多线染色体的变性DNA杂交，放射自显影仪可通过叠印特定纹带的纹理来识别应答基因的位置。

He and Nina moved to California and lived at 2005 Ivar Street, Apt.

他和Nina搬到加州，并在2005年伊瓦尔街，公寓生活。

Droperidol （ potently inhibits transfected HERGchannels and this is the probable mechanism for QT prolongation.

氟哌利多有效地抑制了转染的 HERG 钾通道，可能是 QT 间期延长的机制。