查询词典 hybridized

The total RNA from brain tissue of substantia nigra of PD rats is extracted at the 1st, 2nd, 4th and 8th week and comparison is made between it and that from normal rats. The total RNA, after RNA formaldehyde degenerated sepharose electrophoresis and separation and Northern transfer, is adsorbed unto colloxylin membrane. Then ECL labeled Selenoprotein P and an unknown gene fragment are used as probes to hybridize with the RNA on the colloxylin membrane. And the hybridized result is obtained with enzyme-linked immunosorbent assay. Finally, analysis of OD is made using ONE-Dscan software.

分别提取1周、2周、1月、2月的帕金森大鼠（各8只）黑质区脑组织总RNA，并取相对应时间的正常大鼠的脑组织总RNA作为对照，经RNA甲醛变性琼脂糖凝胶电泳分离后，通过Northern转移，将其吸附在硝酸纤维素膜上，然后利用ECL标记的Selenoprotein P和一段未知基因为探针，与硝酸纤维素膜上的RNA分子进行杂交，再通过显影取得杂交的结果，应用ONE-Dscan软件进行光密度值分析。

Other three alien translocation lines referring to chromosome Lr.14 of L.racemosus were also induced via irradiated pollens.After irradiated by gamma raysof 1KR does,the pollens of T.aestivum-L.racemosus Lr.14 disomic addition lineswere used to pollinate wheat varieties"Yangmai No.5"and"Mianyang No.11".M1plants have pairing between wheat chromosomes and Lr.14 ofL.racemosus at PMCMI stage were self-pollinated,and the M2 seeds used to be Giemsa C-banded andfluorescence in situ hybridized.As the result,three alien translocation lines wereobtained and T12 was observed to have distal translocation chromosomes betweenLr.14S and a wheat chromosome segment(TW-Lr.14S·Lr.14L).T13 wasidentified to contain mostly-alien-translocation chromosome(TLr.14L·Lr.14S-W).T14 was proved to contain a translocation between Lr.14L and 6BS,with approximatebreakpoint at 6BS2.2 region, FL0.22 in the short arm of chromosome T6BL.6BS-Lr.14L.

用普通小麦-大赖草Lr.14二体异附加系经辐射处理过的花粉给扬麦5号和绵阳11号授粉，M1代植株进行PMC MI染色体配对分析和Giemsa C-分带，选出PMCMI小麦和大赖草染色体发生配对的植株进行自交，M2代经RTC M期染色体C-分带和原位杂交，鉴定出3个小麦-大赖草易位系，其中T12为由Lr.14大部分和一个小麦染色体片段组成的易位染色体(TLr.14L·Lr.14S-W)；T13含由Lr.14染色体绝大部分与小麦染色体的一个小片段组成的易位染色体(TLr.14L·Lr.14S-W)；T14易位系的染色体易位发生在小麦6BS2.2区和大赖草Lr.14L之间，易位断点在T6BL·6BS-Lr.14L易位染色体的FL0.22附近。

In addition, the human X-specific and Y-specific whole chromosome painting probes were hybridized onto metaphase spreads obtained from male of Elaphodus cephalophus.No signals were detected, which indicates the phylogenetic relationship between human and the tufted deer is not close.

分别用人X、Y染色体特异的整条染色体涂染探针，通过荧光原位杂交的方法，检测核型Ⅰ中是否存在与人X、Y染色体同源的片段，结果显示该毛冠鹿间期、中期细胞都呈阴性，提示可能毛冠鹿与人的亲缘关系较远。

Inaddition,this probe no only hybridized strongly to the DNA of gastric cancer cell linesand gastric cancer tissue but also to the DNA of other gastroenterological cancer.

这些结果提示，1A360片段可能来自于1个候选的癌基因，它在胃癌组织和细胞系中均见扩增并有相对的组织特异性。

In the GISH, a given genomic DNA was hybridized to chromosomes of their own or other species and signals of repetitive DNA sequences were exclusively detected.

一种新的通过基因组原位杂交进行的分子显带方法，即利用一个物种的基因组DNA作探针，对该物种或其它物种的染色体进行原位杂交，其杂交信号的检出程序是专门用于检出重复DNA顺序的。

These sequences possessed highly copy number in the genome and were named as RMX1-6 individually. When these sequences were hybridized to chromosomes of spiny eel, RMX6 was proved to be sex chromosome specific and it only located in heterochromatic regions.

在随机挑取的三百多个克隆中，分离得到了6个高度重复序列，它们在基因组中存在较高拷贝数，按照发现顺序分别命名为RMX1—6(Repetitive sequence inMicrodissected X chromosome)。

As to'Cabemet Sauvignon'and'Queen of Vineyard', which belong to the'West Europe Cultivars Group', no obvious decline of Fv/Fm was found in their potted plants even under severe drought stress. Their photosynthetic abilities also greatly recovered after rewatering, i. e. no serious photodamage was formed in these cultivars under drought stress. Between them, the φPSⅡ of 'Queen of Vineyard'under severe drought stress was much lower than that of'Cabernet Sauvignon', which suggested that the light use efficiency of the former was much lower than the latter under severe drought stress. The potted plants of'Autumn Royal'and'Rizamat' sustained high Fv/Fm and φPSⅡ before moderate drought stress, but seriously declined under severe drought stress, which indicated that these cultivars had a weak resistant ability to extreme drought stress. A hybridized cultivar of Vitis vinifera L. and V. labrusca L.,'Red Double Taste', its Fv/Fm and φPSⅡ expressed mild decline along drought stress grade.φPSⅡ of'Red Double Taste'was the highest among seven cultivars under severe drought stress condition, which might closely connected with its high photorespiration activity. Another new cultivar,'Red Globe', and a rootstock,'1103Paulsen', the Fv/Fm and φPSⅡ of their potted plants declined markedly under slight drought stress, which suggested that they held a weak ability to defend against photoinhibition.

西欧品种群的赤霞珠和葡萄园皇后，其盆栽苗的Fv／Fm直到重度干旱胁迫下仍没有明显的降低，且复水后光合能力恢复程度较高，即干旱没有给这两个品种造成严重的光破坏，而两个品种之间，葡萄园皇后在重度干旱胁迫下的φPSⅡ明显低于赤霞珠，说明其光合器官在严重干旱胁迫时对光能的利用率较差；新品种皇家秋天和东方品种群的里扎马特在中度干旱胁迫下仍可维持较高的Fv／Fm和φPSⅡ，但在重度干旱胁迫下则降低幅度较大，说明其抵御极端干旱胁迫的能力较差；欧美杂种红双味的Fv／Fm和φPSⅡ随着干旱胁迫程度的增加呈现平缓的下降趋势，而且其φPSⅡ在重度干旱胁迫下是七个品种中最高的，这应该与其此时仍维持较强的光呼吸活性有关；红地球和砧木1103Paulsen的Fv／Fm和φPSⅡ在轻度干旱胁迫下就明显下降，表明干旱条件下其抵御光抑制的能力较差。

M-3-16 and baker's yeast BY-6 haploid strain without using melibiose was hybridized, 153 hybrids were identified.

将M-3-16与不利用蜜二糖的面包酵母BY-6单倍体进行杂交，共获得杂交株153株。

Result The COⅠ probe hybridized well with DNA from Paragonimus westermani, but not with DNA from other species.

将酶切后的COⅠ基因和ITS2基因回收，地高辛标记成探针，尼龙膜上行斑点杂交观察。

The chemical composition, fibre morphology and feasibility of pulping and papermaking of hybridized Chinese pennisetum was studied in a research trial.

对杂交狼尾草的化学成分、纤维形态及制浆造纸的可行性进行了研究试验。

He and Nina moved to California and lived at 2005 Ivar Street, Apt.

他和Nina搬到加州，并在2005年伊瓦尔街，公寓生活。

Droperidol （ potently inhibits transfected HERGchannels and this is the probable mechanism for QT prolongation.

氟哌利多有效地抑制了转染的 HERG 钾通道，可能是 QT 间期延长的机制。