查询词典 genetic engineering

Amon g them , four polymorphic loci with inevident genetic variation made little cont ribution to genetic variation of this population and other ten polymorphic loci with evident genetic variation made great contribution to genetic variation of this population .

其中有14个酶基因座属多态，只有一个单态基因座（ME－3）。14个多态基因座中，4个基因座遗传变异小，对该天然群体的遗传变异贡献不大，其余10个基因座遗传变异丰富，对该天然群体的遗传变异贡献大。

Furthermore, out of 497 fAFLP markers, 80 special bands were found to be able to distinguish the four groups from each other and may be applied for germplasm characterization and molecular assistant classification of Meretrix clam.4 Molecular classification of two species of Meretrix clam based on fAFLP and ITS sequences4.1 The results of fAFLP maker analysis of S, G and W showed that each group had their own specific loci among which there were 53 special loci in W group, much more than those of S group (14) and G group (21). Among the 53 loci, nine were all dominant loci. These unique loci could be taken as molecular markers to distinguish W from other groups. The genetic similarity indexes and distance matrix between S and G groups were 0.9585 and 0.0424 respectively, but the genetic similarity indexes and distance matrix between W group and S or G group was 0.7939 or 0.7941, and 0.2308 or 0.2305 respectively. The results revealed that significant difference existed between W and S or G groups in molecular genetic structure. The phylogenetic trees by the methods of UPGMA and NJ also indicated that S and G populations were very closely related, while W population was a relatively independent cluster, lying beyond the species which S and G belong to.4.2 The internal transcribed spacer region of the rDNA from S group, G group and W group were PCR amplified and sequenced. The results showed that the size of ITS ranged between 1266-1269bp in W group, while those in G and S groups were 1614bp and 1520bp respectively. The GC content ranged 62.32-62.62% in W group while it was 61.77% in G group. The genetic distances between three populations of W group were 0.001～0.003, but it was 0.110 or 0.147 respectively between W group and G group or S group. Phylogenetic trees by NJ method also showed that G group was very closely related to S group, while W group was a relatively independent cluster.

在457个总扩增位点中找出了53个W的特有位点，远多于S群体(14)和G(21)群体，而且在53个特有位点中有9个出现频率为100%的位点，这些位点可以作为区分其它2个群体的特征性标记；S– G群体特有的位点有112个，其中有4个位点出现频率为100%，可作为S– G群体区别于W群体的特征性标记。S群体和G群体间的遗传相似性系数为0.9585，遗传距离只有0.0424，在NJ和UPGMA法构建的亲缘关系的树状图上均首先聚在一起，说明二者的亲缘关系很近，应属于种内群体间的关系；而W与S和G的遗传相似性系数均较小(0.7939和0.7941)，相对遗传距离很大而且十分相近(0.2308和0.2305)，在亲缘关系树状图上单独分出一支，也表明W与S和G群体间的亲缘关系较远。4.2 ITS序列比较分析通过对白壳文蛤、山东文蛤和广西文蛤的ITS序列扩增电泳、PCR-RFLP分析和ITS序列分析发现，W的ITS序列长度在1266-1269 bp，而S和与G的ITS序列总长度分别为1520 bp和1614 bp；从ITS1和ITS2长度来看，W分别为739-741 bp和316-317 bp，S为895 bp和414 bp，G为987 bp和416 bp；而从ITS碱基组成来看，W的GC含量在62.32-62.62%之间，而G群体为61.77%。W的3个壳色不同群体间的遗传距离仅0.001、0.002和0.003，S与G群体间的遗传距离是0.010，说明W群体内变异很小，而S与G群体间已出现明显的遗传分化，但还均属于种内群体间的遗传变异；而W与G和S的遗传距离分别达到0.110、0.147，两个类群差异显著，已远超出种内群体间的遗传变异。

Based on the genetic distance of 26% by SSR data, the cluster analysis with un-weighted pair-group method using arithmetic averages produced 2 major clusters: cluster Ⅰ of higher degrees of genetic differentiation and Cluster Ⅱ of lower degrees of genetic differentiation relatively, which are composed of 30 cultivars, and another cluster of much higher degrees of genetic differentiation, which is only composed of the cultivars of FHIA 25 and Pisang Jari Buaya.

依据26%的遗传距离，除了FHIA25和Pisang Jari Buaya单独化成1组外，其它30个品种可以分为2组：品种间遗传差异相对较高的组Ⅰ和品种间遗传差异相对较低的组Ⅱ。

ISSR markers were used to study the genetic relationship and investigate the genetic diversity and genetic structure for providing a theoretical basis and technical support for appropriate conservation and application of existing genetic resources of Tagetes.

通过ISSR分子标记研究品种资源遗传关系，探讨其种群遗传多样性水平和遗传结构，为了解品种间遗传多样性、品种间亲缘关系、育种及科学合理保存和利用现有万寿菊属种质资源提供理论依据和技术支持。

The model was based on genetic tabu algorithm of global search and BP global network of local search precision features, combined genetic tabu algorithm and BP algorithm, and used genetic tabu algorithm initial weights of BP network, at the same time,used niching technology to improve genetic tabu algorithm.

该模型基于遗传禁忌算法的全局搜索和BP网络局部精确搜索的特性，将遗传禁忌算法和BP算法有机结合，利用遗传禁忌算法优化BP网络初始权重，同时引入小生境技术改进遗传禁忌算法。

Anticipated results of this core laboratory are to:(1) facilitate genetic epidemiological studies within the campus by means of active avocations, workshop, and collaboration;(2) help identify important genetic variants as well as contextual variables that influence the risk of metabolic syndrome in a community sample of large sample size;(3) help the gene mapping for schizophrenia by means of family-based association as well as schizophrenia-related endophenotype in non-clinical samples;(4) help train more students and research assistants who can deal with genetic epidemiology via hands-on experience; and (5) help researchers accomplish their genetic epidemiological investigation of the diseases they are interested in.

本核心实验室之预期结果为：(1)藉由主动推广、工作坊、研讨会等方式促进遗传流行病学研究；(2)对於社区样本中常见之代谢症候群，可协助找出相关的基因变异以及脉络因素；(3)藉由家族关联研究可协助对於精神分裂症基因定位分析，并探讨其内表型在非临床样本中的特性；(4)协助学生、助理亲手处理遗传流行学学分析的训练；(5)协助研究人员完成他们对於感兴趣疾病的遗传流行病学研究。

One day an infant born with a genetic defect of the bone marrow or blood may be able to have his umbilical cord blood harvested at birth, repaired by genetic engineering and then reinfused, so that he need never suffer the negative effects of his genetic inheritance.

有朝一日，骨髓或血液发生遗传缺失的婴儿，都可用出生时收集的脐带血，以基因工程修复后再输回体内；这样一来，他一辈子都不必为遗传疾病所苦。

Results After six passages in BHK-21 cells, cytopathic effect was observed by microscopy. The rescued virus was rejuvenated once in unweaned mice, and then came back to cell passage. We found that the poly tract of rescued virus was shortened when the virus was passaged twice in BHK-21 cells. Although the data of LD50 in mice and TCID50 in BHK-21 cells showed that the virulence and infectivity of genetic engineering viruses were lower than its parental virus, no significant difference was observed between the genetic engineering viruses with the different length poly tract.

我们发现，基因工程毒在BHK-21上连续传代至第6代时，可见明显的致细胞病变效应(cytopathic effect，CPE)；在经过一代乳鼠接种之后再重新适应到BHK-21细胞的过程中，poly区段出现了缩短现象；乳鼠致病性试验和BHK-21细胞TCID50测定结果表明，尽管基因工程毒与母本毒相比毒力偏低，但含有不同长度poly的基因工程毒之间并无显著差异。

The courses in this academic program are political theory, foreign languages, applied computational technology, experimental biology, microbiology, molecular biology, genetic engineering, bioseparation engineering, bioreaction engineering, chemical thermodynamics, mass transfer, chemical process simulation, pharmacology, preparation of finished traditional Chinese medicines, pharmaceutical chemistry, GMP in pharmaceutical industry, liquid-to-liquid extraction, pharmaceutical properties measurement and quality control, pharmacy administration, etc.

研修的主要课程有：政治理论课、外语课、数值分析、计算机应用技术、现代生物学与技术实验、现代微生物学、分子生物学、基因工程、生物分离工程、生物反应工程、化工热力学、化工传递过程原理、化工过程模拟、药理学新进展、中成药的制备、药物化学的进展、制药工业的 GMP、液液萃取基础、药品性能检测与质量控制、药业管理学等。

The courses in this academic program are political theory, foreign languages, applied computational technology, experimental biology, microbiology, molecular biology, genetic engineering, bioseparation engineering, bioreaction engineering, chemical thermodynamics, mass transfer, chemical process simulation, pharmacology, preparation of finished traditional Chinese medicines, pharmaceutical chemistry, GMP in pharmaceutical industry, liquid-to-liquid extraction, pharmaceutical properties measurement and quality control, pharmacy administration, etc.

其工程硕士学位授权单位培养从事生物制药、合成制药、中药开发、药剂、药物性能检测与分析的高级工程技术人才。研修的主要课程有：政治理论课、外语课、数值分析、计算机应用技术、现代生物学与技术实验、现代微生物学、分子生物学、基因工程、生物分离工程、生物反应工程、化工热力学、化工传递过程原理、化工过程模拟、药理学新进展、中成药的制备、药物化学的进展、制药工业的GMP、液液萃取基础、药品性能检测与质量控制、药业管理学等。

Warden: That's a beautiful dog, but don't bring her again.

那是条漂亮的狗，但是不要再把她带来了。

You get up in the morning – no hangover!

你第二天早上起来的时候—不会宿醉！