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The release of CRH in stress response during menopause inhibited the secretion of GnRH, gonadotropic hormone and estrogen and induced the expression of intracellular HSP. As the growing of month, the expression of HSP70 and HSP70mRNA in uterus went up at the beginning and came down in the end. The result might be caused by the following:The increase of CRH and the decrease of estrogen during early menopause weakened the inhibitory effects on HSP.

本实验观察到随着大鼠月龄的增长子宫HSP70及mRNA的表达呈先增后降的变化，早期热休克蛋白的增加可能与CRH的增加及局部雌激素水平下降对HSP抑制作用的降低有关，但随着组织器官的衰退，其蛋白合成能力下降，因而表现出晚期热休克蛋白及基因表达下调。

Methods Realtime fluorescence quantitive RTPCR was used to detect the expression level of Tiam1 mRNA in gastric cancer tissues of 66 cases, paracancerous tissues (over 3cm) of 66 cases and gastric benign lesion tissues of 11 cases. Meanwhile, CK18 was selected as the hallmarker for lymph node micrometastasis to detect the level of CK18 mRNA expression in 199 lymph nodes from 24 cases of gastric carcinomas diagnosed negative by pathologic examinations.

采用TaqMan探针实时荧光定量PCR方法，定量检测66例胃癌患者癌组织、癌旁组织及11例胃良性病变组织中的Tiam1 mRNA拷贝数，同时将CK18作为淋巴结微转移的标志基因，定量检测24例病理诊断无淋巴结转移的胃癌患者199枚淋巴结的CK18mRNA拷贝数。

Objective To construct spinocerebellar ataxia 3 (SCA3) eukarotic expression vector with en hanced green fluorescent protein as report gene and to observe its expression in PC12 cells.

目的 构建以增强型绿色荧光蛋白作为报告基因、带有脊髓小脑性共济失调3型(SCA3)基因的真核细胞表达载体，并转染PC12细胞，观察外源基因的表达情况。

OBJECTIVE: To compare the differences of gene expression and the response to bFGF of human umbilical cord CD34+ and CD133+ cells, and to explore gene expression changes of bFGF-induced umbilical cord CD34+ and CD133+ hematopotic stem cells/hemapoietic progenitor cells in vitro.

目的：利用基因芯片比较脐血CD34+和CD133+细胞基因表达的差异，并探讨碱性成纤维细胞生长因子体外诱导脐血CD34+和CD133+造血干/祖细胞分化的基因表达变化，及其对碱性成纤维细胞生长因子反应性差异。

ConclusionHCMV infection significantly down-regulated the expression of HOXB6 gene.The result suggests that the abnormal expression of HOXB6 gene induced by HCMV may play an important role in HCMV-induced abnormal hematogenic damage.

结论HCMV感染能诱导脐血红系祖细胞HOXB6的表达下调，HCMV有可能通过调控HOXB6基因表达异常而引起红系祖细胞的增殖障碍。

Selenium level in HP-infected gastric mucosal lesions Was negative correlated significantly with Expression rat of CD44V6, C-met and PCNA ( P.05 ), but was positive correlated with expression rat of P16 (P.05). Conclusions Selenium can influence morphological conversion, and decrease hematolysis and effects the function of vacuole toxin of HP.

其原因可能是机体为保护性地对抗幽门螺杆菌感染所致增加的活性氧而动员硒，使组织硒含量增加，但由于随胃黏膜组织病变程度加重，硒消耗增多，使含量又相对降低；硒含量分别与CD44V6、C-met、PCNA表达阳性率呈负相关，而与P16表达阳性率呈正相关。

Decreased expression of MMPs by blocking the expression of Snail in ovarian carcinoma cells Objective: To investigate the influence of antiadhesive antibody (SHE78-7), targeted at the potent hemophilic cell adhesion molecule E-cadherin, on the motility and invasive ability of ovarian carcinoma cells that have stably tansfectant with anti-Snail plasmid.

第二部分反义Snail对裸鼠卵巢癌原位移植瘤转移的影响目的：观察转染反义Snail质粒的卵巢癌细胞在裸鼠原位移植瘤模型中生长及转移的情况，检测转移组织中Snail、E-钙粘蛋白、及基质金属蛋白酶的表达和分布。

Fibroblasts have the ability to secret many kinds of extracellular matrix and cytokines relevant to hepatogenic differentiation. So, we investigate whether coculture of hADSCs with fibroblasts without cell-cell contact play a role in hepatic differentiation of hADSCs by RT-PCR analysis of hepatic specific gene expression and real-time PCR analysis of Alb expression.

成纤维细胞能够分泌很多利于干细胞向肝细胞分化的细胞外基质和细胞因子，我们采用非接触共培养的方式对成纤维细胞与人脂肪干细胞共培养能否引发其向肝细胞的分化进行了研究。

Further, the global gene expression profiles differ in hepatoma cells with and without HBV gene expression and replication.

此外，在肝癌细胞系中，乙肝病毒的复制和乙肝蛋白的表达能引起细胞基因表达的全局性改变。

There is Cx43 expression in the human thyroid epithelial cells, and there are heterogenicity in levels of Cx43 expression in autoimmune thyroid diseases.

Cx43可在人甲状腺上皮细胞中表达；Cx43在AITD的表达强度存在异质性，这种缝隙连接蛋白表达的异质性可能与AITD的发生、发展及预后有关。

We have something to do in the new stage.

在这段崭新的时期中，我们有一些要做的。

The difference between tourism decision-making under uncertainty and risky tourism decision-making is indistinctive.

其中不确定性旅游决策和风险旅游决策的界线比较模糊，两者的区分是相对的。