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Complementary DNA encoding 49KD ES antigen of Trichinella spiralis muscle larvae was cloned in E.coli by recombinant DNA technology.

应用DNA重组技术将编码旋毛虫肌幼虫49KDa抗原的基因克隆于大肠杆菌中。

The purpose of this thesis is to accomplish encoding and decoding of the extened Golay code and the 1/2 convolutional code with Xilinx FPGA applicable in the trunked system.

本论文的目的是对扩展Golay码和卷积码在集群通信中应用及其FPGA实现的研究。

Site-directed mutagenesis was used to change the gene encoding tryptic bovine liver microsomal hepatic Cyt 〓.

本论文采用寡聚核苷酸定点突变技术对人工合成的编码牛肝细胞色素〓的基因进行改变，得到了目标突变基因。

The Oryza sativa genome contains at least 6 uncharacterized encoding proteins with a SPX domain at the N-terminal portion.

水稻中包括至少6个在N-末端含有SPX结构域的未知功能的蛋白。

This implies that DNA methylation of promoter exon 1_7 may not underly the mechanism of GR up-regulation in GLP-1 -programmed rats.5 Hippocampal expression of GLP-1R and NGFI-A mRNAHippocampal GLP-1R (p =.009) and NGFI-A (p =.001) gene mRNA expression were significantly up-regulated in GP group as compared to VP group.Taken together, neonatal intramuscular injection of plasmid DNA encoding GLP-1 affects behavior and hippocampal GR expression in adolescent rats.

提示GR exon 1_7启动子区域DNA甲基化可能不参与GLP-1导致的GR转录上调。6海马GLP-1R和NGFI-A mRNA表达GP组大鼠GLP-lR(P=。009)和NGFI-A(P=。001)表达均显著高于VP组，表明肌肉注射GLP-1可能通过作用于其海马受体提高NGFI-A表达，进而调控GR表达。

BACE algorithm has the desired property that does not reduce the encoding efficiency of the CABAC entropy encoder compared to the CIME algorithm for CAVLC entropy encoder. Both CIME and BACE algorithms can adapt to the content security level by choose the amount and different key parts of the video stream to be encrypted, the theoretical and experimental results show that both these algorithms can effectively encrypt the video content. Due to the ability of encrypting the video content without violating the syntax, the BIME and BACE algorithms can be employed used for many advanced H.264/AVC coding options without first decrypting the video stream, like Data Partition and UnEqualized Protection for the enhancement of transmission robustness, and the scalable coding in order to match the different channel capacity.

上述两种结合了H.264/AVC熵编码器特征的内容选择性加密算法，由于其加密后的视频码流的句法结构完全符合H.264/AVC视频编码标准，与传统的基于块数据的块加密算法相比较，本文提出的两种内容加密算法可以使得加密后码流在无需解密的情况下，实现H.264/AVC视频编码标准为移动视频应用而设计的诸多特性，如：增强传输鲁棒性的数据分割技术和非均衡保护技术，以及直接做匹配信道带宽的分级编码操作等，具有更高的灵活性和计算效率，从而可以灵活运用于各种应用场合。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Unsuppressed water reference signal from the volume of interest is relatively unaffected by these artifacts and is collected without phase encoding.

有关体积未抑制的水基准信号受这些膺象的影响不大，因而无需相位编码即可以收集。

For the inherent untunable interaction between qubits, concatenating BB pulse control with DFSs encoding can eliminate all the unwanted effect of the interaction.

对于量子比特之间固有的不可调相互作用，通过级联bang-bang脉冲控制和无消相干子空间编码会消除固有相互作用破坏量子信息的的不良效应。

Thedegenerate primers were designed and synthesized according to the sequence of NDHB amino acid published in GneBank. A novel cDNA encoding the second subunit of NADH dehydrogenase was amplified from Fraxinus velutina Torr by RT-PCR.

根据GenBank上已发表的NDHB氨基酸序列，设计合成了一对简并引物，采用RT-PCR技术对绒毛白蜡NADH还原酶第二亚基基因进行扩增，将扩增的PCR产物与pMD18-T连接后转化至E。

Pachomius manual labour was organized as an essential part of the monastic life; and since it is a principle of the monks as distinguished from the mendicants, that the body shall be self-supporting, external work of one sort or another has been an inevitable part of the life ever since.

根据圣pachomius体力劳动组织作为一个重要组成部分寺院生活；以来，这是一个原则的和尚，作为有别于乞丐，该机构应自支撑，对外工作的这种或那种已一个必然的一部分，生活至今。

In order to study the effects of parametrical rolling on the ship movement and tilts, in allusion to ship's navigating in regular following waves, the equation of ship rolling motion is transformed into normal Mathieu equation by proper variable replacement when strong parametrical excitation in restoring moment varied according to sine or cosine wave.

为研究参数横摇对船舶运动和倾覆的影响，通过对船在规则波上随浪航行时，恢复力矩中出现的1个以正余弦规律变化的强参数激励项进行适当的变量置换，将船舶的横摇运动方程转换成标准形式的Mathieu方程。