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For better understanding of the phylogenic diversity and evolution of FSH p molecule, we have isolated and sequenced the genomic sequence encoding Chinese swamp buffalo FSH p precursor molecule by PCR.

为了更好地了解FSHβ基因的进化情况，我们利用PCR方法对中国沼泽型水牛的该基因的基因组序列进行了克隆测序。

The nucleotide sequences of the PAP gene and PAPⅡ gene in the root of Phytolacca americana were determined by reverse transcription polymerase chain reaction and PCR. The gene comprises 942bp and 933bp respectively, encoding 313 amino acids and 310 amino acids. When PAP gene and PAPⅡ gene sequences in the root were compared with those in the spring leaves and summer ones, the highest similarity 100% and 99. 8% were observed respectively. They both had one open reading frame . Analysis of the two sequences showed, in fact, they belonged to one species.

以美洲商陆根为材料，提取其总RNA、反转录，用一对PAP基因的特异性引物和一对PAPⅡ的特异性引物，分别对反转录产物进行PCR扩增，将扩增的目的片段进行序列分析，结果扩增出来的PAP基因与已经报道的序列完全相同，扩增出来的PAPⅡ基因与已经报道的序列同源性也在99.8％，而且它们都各自编码一个开放阅读框架，可以说美洲商陆根中肯定存在PAP和PAPⅡ基因，并已经转录。

The nucleotide sequences of the PAP gene and PAPII gene in the root of Phytolacca americana were determined by reverse transcription polymerase chain reaction and PCR. The gene comprises 942bp and 933bp respectively, encoding 313 amino acids and 310 amino acids. When PAP gene and PAPII gene sequences in the root were compared with those in the spring leaves and summer ones, the highest similarity 100% and 99.8% were observed respectively. They both had one open reading frame. Analysis of the two sequences showed, in fact, they belonged to one species.

以美洲商陆根为材料，提取其总RNA、反转录，用一对PAP基因的特异性引物和一对PAPⅡ的特异性引物，分别对反转录产物进行PCR扩增，将扩增的目的片段进行序列分析，结果扩增出来的PAP基因与已经报道的序列完全相同，扩增出来的PAPⅡ基因与已经报道的序列同源性也在99.8％，而且它们都各自编码一个开放阅读框架，可以说美洲商陆根中肯定存在PAP和PAPⅡ基因，并已经转录。

In this study, the cDNA fragments encoding α subunit of nicotinic acetylcholine receptor was cloned from the diamond back moth, Plutella xylostella with RT-PCR techniques. The amplified fragments are 425 nucleotides in length, from which 141 amino acids were deduced.

测序结果显示，扩增的目的基因片段包含了nAChRα亚基的三种亚型，分别命名为Pxal、Pxa2、Pxa3，都具有典型的α亚基的结构：两个相邻的半胱氨酸；形成胞外环的15个氨基酸；与烟碱和α银环蛇毒素结合有关的氨基酸残基。

This research consolidated that evolutionary models using w as an indicator of selective pressure provide valuable approaches for analyzing the adaption of protein-encoding sequences. Moreover, it also indicated that Polypodiaceous ferns responded to the terrestrial ecosystem changes initiated by angiosperms through molecular adaption of functional genes such as rbeL.

这些结果一方面显示了基于ω比值检验DNA编码序列分子适应的有效性，另一方面也提示水龙骨类可借助rbcL等功能基因的适应性进化，应对白垩纪被子植物引发的陆地生态系统改变。

The negative encoding of the social information processing o f the students deˉpressive symptoms was mainly caused by the negative bias of p rocessing of the positive items and the neutral items.

提示抑郁症状中学生对正性和中性的社会信息较多的负偏向编码。

Co-expression of collagen genes and cDNAs encoding the subunits of prolyl hydroxylase has lead to the synthesis of completely hydroxylated, thermostable collagens.

胶原基因和编码羟化酶亚单位的 cDNAs 的共表达已经可以使胶原完全的羟基化并耐热。

During encoding, the right amygdala and right pulvinar were activated when subjects unconsciously encoded fearful faces (vs. neutral faces).

在编码阶段，与中性面孔相比，恐惧面孔在无意识编码的被试中激活了右侧杏仁核和右侧枕核；而在有意识编码的被试中激活了左侧杏仁核。

Effective encoding technologies to obliterate redundance and retain the image quality are the focus of research.

采用有效的压缩编码技术删除冗余，以尽量少的比特数存储图像，并同时保证图像的质量，己成为研究的热点。

To express chicken osteoprotegerin in Pichia pastoris and determine its inhibitive effects on mature osteoclastic bone resorptive function in vitro. The chOPG cDNA encoding for 382 amino acid without signal peptice was subcloned into Pichia pastoris expression vector pPICZα-A. The constructed plasmid was transformed into yeast X-33 by electroporation. The recombinant transformants were selected by Zeocin. Induced by the addition of methanol every 24 hours, the product analyzed by SDS-PAGE was sized about 43kDa and 53kDa at a yield of 200mg per litter of culture. The result of Western blotting indicated that the recombinant protein had specific antigenicity mainly owing to heterogeneous glycosylation.

为了在毕赤酵母中分泌表达鸡骨保护素（osteoprotegerin，OPG），采用DNA重组技术将chOPG成熟肽cDNA片段插入毕赤酵母表达载体pPICZα-A中，以电穿孔法转化酵母X-33，用Zeocin 平板筛选重组子，经甲醇诱导表达后， SDS-PAGE 和免疫印迹分析表达产物，由于糖基化不同，表达产物有两种，其相对分子量分别为43kDa和53kDa，表达量约为200mg/L，经Western印迹验证，有较好的抗原性。

Pachomius manual labour was organized as an essential part of the monastic life; and since it is a principle of the monks as distinguished from the mendicants, that the body shall be self-supporting, external work of one sort or another has been an inevitable part of the life ever since.

根据圣pachomius体力劳动组织作为一个重要组成部分寺院生活；以来，这是一个原则的和尚，作为有别于乞丐，该机构应自支撑，对外工作的这种或那种已一个必然的一部分，生活至今。

In order to study the effects of parametrical rolling on the ship movement and tilts, in allusion to ship's navigating in regular following waves, the equation of ship rolling motion is transformed into normal Mathieu equation by proper variable replacement when strong parametrical excitation in restoring moment varied according to sine or cosine wave.

为研究参数横摇对船舶运动和倾覆的影响，通过对船在规则波上随浪航行时，恢复力矩中出现的1个以正余弦规律变化的强参数激励项进行适当的变量置换，将船舶的横摇运动方程转换成标准形式的Mathieu方程。