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The cDNA was 919bp in length and contained a complete Open Reading Frame of 576bp, encoding a protein of 192 amino acid residues. The deduced amino acids of OsRPS7 showed 88%、72% and 72% identity with those from Secale cereale、Arabidopsis thaliana and Brassica oleracea, respectively.

该cDNA序列全长919bp，编码192个氨基酸；其与黑麦、拟南芥和芸薹的S7核糖体蛋白的氨基酸一致率分别为88%、72%和72%。

Transcription/translation assay and transient transfection of COS-7 cells with a cDNA encoding Sparus aurata lier GK showed the functionality of the enzyme in itro.

转录和翻译水平的分析，以及用金头鲷肝脏GK的cDNA转染COS－7细胞都表现出GK在体外的活性。

Methods At first, retrovirus vectors encoding IFN-γ or IL-4 gene were constructed. They were transfected into PA317 packaging cells by lipofectamine, PA317IFN-γ and PA317IL-4 cells were obtained. C6 glioma cells were infected with replication deficiency retrovius containing IFN-γ or IL-4 gene, cell morphology、cell growth curve and cloning efficiency assay were examined. The intracranial C6 glioma animal model was established in immunocompetent Wistar rats. PA317IFN-γ and PA317IL-4 cells were stereotactically implanted into the tumor areas alone or together. The survival of tumor bearing rats were examined, tumor volumes were measured and immunohistochemical analyses of CD4〓 and CD8〓 T lymphocyte infiltration were performed.

构建和鉴定携带IL-4或IFN-γ基因的逆转录病毒载体，将其导入逆转录病毒包装细胞PA317，通过G418抗性筛选，得到携带目的基因的包装细胞并鉴定；应用携带IL-4或IFN-γ基因的复制缺陷型逆转录病毒感染C6胶质瘤细胞，观察对C6胶质瘤细胞形态、细胞生长曲线和克隆形成率的影响；建立C6胶质瘤大鼠脑内移植动物模型，将携带IL-4和IFN-γ基因的逆转录病毒包装细胞分别或联合注射到脑内荷瘤大鼠的肿瘤组织中，观察其治疗作用，并初步探讨其机制。

Then rAAV-DJ-1 was transfected into HEK-293 cells for packaging. AAV vectors encoding human DJ-1 protein were stereotactically injected into the rat SN 4 weeks prior to rotenone lesion in SN. Tyrosine hydroxylase protein was determined using immunohistochemical staining. Ethovison software allowed measurement of movement distance in 30 minutes.

并转染HEK-293细胞，进行腺相关假病毒颗粒包装，所得病毒颗粒注射到大鼠脑内感染黑质神经细胞；4周后，注射鱼藤酮于大鼠黑质相同脑区；通过免疫组织化学方法鉴定TH蛋白表达情况，采用动物行为轨迹分析软件计算大鼠30 min内运动距离。

Lame392和RozarLame115-LAME is undoubtedly the best MP3 encoding software with the traditional MP3 encoder than it can make better use of people's ears shadowing effect, support VBR and the joint stero, so in a certain compression ratio to achieve a better listening effect.

LAME无疑是目前最优秀的MP3编码软件，与传统的MP3编码器相比，它能够更好地利用人耳遮蔽效应，并且支持VBR以及joint stero技术，从而在一定的压缩比达到了更好的听音效果。

Subsequently, a l kb fragment of HCCR-2 encoding region was amplified by RT-PCR and cloned into Promega TEasy vector.

测序证实无误后，以HCCR-2／T载体为模板，再通过PCR克隆出HCCR-2的编码区序列，将其连接到pIRES2-EGFP真核表达载体，并通过测序获得证实。

Results: The anti PSA scFv/human p53 tetramerization domain fusion gene consisted of 891bp encoding 297 amino acid residues, and was the same as that reported before. The expression products of the tetrameric anti PSA scFv, which relative molecular mass was about 35 000, were confirmed by SDS PAGE and Western blot.

结果：获得了抗PSA scFv/人p53四聚功能域融合基因，基因全长891bp，可编码297个氨基酸，与已发表的抗PSA，scFv，人IgG3上游铰链区和人p53四聚功能域基因cDNA序列一致。

Objective: Detect mRNA encoding TSHR in retroorbital fatty and connective tissue with TAO and healthy subjects, demonstrate that TSHR may present in retroorbital tissue and serve as common antigen in the thyroidal and extrathyroidal manifestations of TAO.

目的：寻找在甲状腺外组织TAO患者球后脂肪结缔组织中编码促甲状腺激素受体的mRNA，证实TSHR作为甲状腺和TAO球后脂肪结缔组织的共同抗原而存在于甲状腺外结构中。

Objective To obtain antigenic genes encoding newborn larvae antigens of Trichinella spiralis.

目的 获取具有抗原性的旋毛虫新生幼虫基因。

Some cDNAs encoding antigenic protein of newborn larvae of Trichinella spiralis were obtained.

获得具有抗原性的旋毛虫抗原编码cDNA分子

Pachomius manual labour was organized as an essential part of the monastic life; and since it is a principle of the monks as distinguished from the mendicants, that the body shall be self-supporting, external work of one sort or another has been an inevitable part of the life ever since.

根据圣pachomius体力劳动组织作为一个重要组成部分寺院生活；以来，这是一个原则的和尚，作为有别于乞丐，该机构应自支撑，对外工作的这种或那种已一个必然的一部分，生活至今。

In order to study the effects of parametrical rolling on the ship movement and tilts, in allusion to ship's navigating in regular following waves, the equation of ship rolling motion is transformed into normal Mathieu equation by proper variable replacement when strong parametrical excitation in restoring moment varied according to sine or cosine wave.

为研究参数横摇对船舶运动和倾覆的影响，通过对船在规则波上随浪航行时，恢复力矩中出现的1个以正余弦规律变化的强参数激励项进行适当的变量置换，将船舶的横摇运动方程转换成标准形式的Mathieu方程。