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The cDNA encoding AChE from susceptible and propoxur-resistant strains of Musca domestica had been cloned using RT-PCR.

2利用RT-PCR技术克隆了家蝇敏感和残杀威抗性品系的乙酰胆碱酯酶基因。

The cDNA sequence encoding ubiquitin from Musca domestica was cloned by RT-PCR and sequenced.

本研究根据GenBank已登录的真核生物泛素编码框的氨基酸序列，设计一对简并引物，RT-PCR克隆了家蝇Musca domestica泛素基因的编码区cDNA序列，并进行了测序。

The result shows that there were two transcriptions in Muscovy duck, MSTN-1(EU336991) and MSTN-2(EU336992). MSTN-1 and MSTN-2 were 1128 bp and 754 bp separately, with homology up to 94%. MSTN-2 included an open reading frame, encoding 124 amino acids.

结果表明，番鸭体内存在2种MSTN基因转录本，MSTN-1(EU336991)和MSTN-2(EU336992)，大小分别为1128 bp和754 bp，同源性为94%；且MSTN-2是一个全新的MSTN转录本，含有1个开放阅读框，可编码124个氨基酸。

Hexon protein gene of egg drop syndrome virus, named strain E05 which was isolated from breeding muscovy duck with irregular egg dropping was amplified by PCR and cloned into pMD18-T vector. The recombinant plasmids were identified by digested restriction endonuclease and PCR. The result showed that the nucleotide length of hexon protein gene encoding 910 amino acids was 2733 bp.

运用PCR技术从表现产蛋异常的种番鸭分离鉴定的减蛋综合征病毒 E05株中分段扩增六邻体蛋白基因，分别与克隆载体pMD18-T连接后，转化感受态细胞，对经PCR鉴定和酶切鉴定均为阳性的克隆进行测序，结果表明，E05株六邻体蛋白基因长度为2733 bp，共编码910个氨基酸。

In order to compare the homology of the gene encoding VP2 between MDPV-Q and MDPV which was registered in the GenBank, also in order to find out changes of the VP2 gene and the immuno-genicity between the wild-strain MDPV-Q and the attenuated strain MDPV-26 which derived by continued passage of virulent wild-type in muscovy duck embryo, a pair of primer (LHMP7/LHMP8) was designed. The upper one LHMP7 and the lower one LHMP8 corresponded to the MDPV-specific nucleotides sequence 2885-2900 and 4618-4604 respectively, according to the MDPV nucleotide sequence databases. The length of the sequence embraced by the primers was 1734bp.

为了获取这一基因片段与国外分离株进行同源性比较，同时也为了了解MDPV强、弱毒株VP2基因之间的异同关系，及其免疫原性的变化规律，通过DNA重组技术，设计了一对引物LHMP7／LHMP8，该对引物选取位于2885～2900及4618～4604的两段序列，跨幅为1734bp，并在这两条引物中分别加入两种限制性核酸内切酶SacⅡ和KpnⅠ的酶切位点，分别对MDPV-Q和由该株病毒经人工致弱后得到的MDPV-26株进行PCR扩增，并将PCR产物克隆到pMD18-T载体上，分别得到二个重组子：pMD18-T—M-Q VP2和pMD18-T-M-26 VP2。

A new data encoding method is presented in this paper.

文中提出了一种新的数据编码传送方法。

Finally, we discuss the encoding methods of mathematical analysis in NL and also in PVS, which is the starting point of mechanical supporting of mathematical analysis in hybrid systems.

最后我们讨论了数学分析在邻域逻辑和PVS中的编码，这是对混合系统数学分析提供机械支持的基石。

Within the hippocampus, genes encoding two neural signaling chemicals, the neuropeptides Grp and Nmb, are expressed in two distinct areas, suggesting functional differences between the two parts.

在海马区，基因编码了两种神经信号化学传导物质，即神经肽Grp 和 Nmb，他们在两个不同的区域里表达，并显示了不同的功能。

In our previous STM studies we found that the disruption of the hns gene encoding a histone-like nucleoid structuring protein could enhance biofilm formation of APP.

我们在筛选APP转座突变体库时，发现hns基因缺失突变可显著增强APP血清1型生物被膜的形成，该基因编码一种DNA结合蛋白。

In our experiment, the GADes gene from rat brain was cloned and expressed in E.coli, so as to make the recombinant GADes produced by gene technology for clinical application and further study on the relation between GAD and type 1 diabetes mellitus.Firstly, with the pUClS/GADes recombinant plasmid as template, the full-length encoding sequence of GADes was amplified by PCR, and cloned into pGEM-T vector. Secondly, the target gene was cut by EcoRI and inserted into restriction sites of pET42a vector for expression.

本研究首先用PCR方法，以pUC18/GAD_(65)为模板，扩增目的基因，克隆到pGEM-T载体中，构建pGEM-T/GAD_(65)重组质粒，然后用EcoRI酶切，切下的目的基因片段插入pET42a融合型表达载体中，经酶切鉴定和测序，证实插入方向、读码框架正确；重组表达质粒转化大肠杆菌BL21(DE_3)中诱导表达，表达产物经亲和层析纯化后获得了融合蛋白GST-GAD_(65)。

Many will continue to choose to live in duality and in conflict.

许多人将继续选择活在二元对立性和冲突中。

I find that students of the University of Physical Education all wear sportswear at first sight.

我发现：体育大学的学生乍一看，都是穿运动衣，大家都一样