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Falciparum isolates prepared directly from 5 cases of cerebral malaria patients'blood in Mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding the regions 12—17 in MAD20 merozoite surface protein 1(MSP1) of Papua New Guinea strain of P . falciparum were used as primers. The PCR products were digested with EcoRI and KpnI, respectively, and the generated fragment regions 16—17 were cloned into M13mp18 and M13mp19 vectors and their DNA were analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应技术对中国5 例脑型疟患者恶性疟原虫云南省勐腊县勐罕CMH/YN 分离株和云南省盈江县农场CYJ/YN 分离株基因组DNA 裂殖子表面蛋白1 (MSP1)第13—17 区基因进行扩增,将扩增产物分别经EcoRI 和KpnI 双酶切后,回收的MSP1 第16—17 区基因分子定向克隆M13mp18 和M13mp19 载体,按Sanger 双脱氧链终止法进行DNA 序列测定,并与MAD20、K1 和Wellcome 株原型基因进行同源性分析比较。

Methods Genomic DNA samples of two isolated Plasmodium falciparum isolate strains prepared directly from 5 cases of cerebral malaria patients′ blood in mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding FC27 merozoite surface protein 2 (MSP2) of Papua New Guinea strain of Plasmodium falciparum were used as primers. The PCR products were digested with BamH1 and Hind Ⅲ respectively, and the generated fragment MSP2 were cloned into M13mp18 and M13mp19 vectors and their DNA was analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应对5例中国脑型疟患者恶性疟原虫云南省勐腊县勐罕分离株和云南省盈江县农场分离株基因组裂殖子表面蛋白2(MSP2)基因进行扩增,将扩增产物分别经BamHI和Hind Ⅲ双酶切后,回收的MSP2基因分子定向克隆M13mp18和M13mp19载体,按Sanger双脱氧链终止法进行DNA序列测定,并与恶性疟原虫株FC27、K1、IC1和CAMP株进行同源性分析比较。

Uses "Specific Area Message Encoding" to provide you with appropriate weather alerts for your neighborhood.

它还使用"特别区域信息编码"向你发送附近地区的天气警报。

After kth encoding they exchange their message.

第k编码后,他们交流他们的信息。

Compared to MSM,method of plasmid elimination,it was proved that the genes encoding for degradation of phenanthrene locate on the larger one of the two plasmids.

与无机盐培养基相比,LB和牛肉膏蛋白胨培养基能促进此菌对菲的降解,尤其能促进对中间产物的进一步转化。

All results from morphological, physiological and biochemical characterization, the 16S rDNA sequence and phylogenetic assay identified that the strain belongs to Micrococcus luteus. The DNA fragment encoding mature peptide of fibrinolytic enzyme was cloned by PCR and sequenced.

利用传统细菌分类鉴定方法对其形态和生理生化特征进行了研究,发现其与藤黄微球菌的特征相符, 16S rDNA序列分析的结果也表明它与藤黄微球菌的16S rDNA序列的同源性高达99%,因此该菌在系统分类学上属于微球菌属藤黄微球菌。

An exquisite encoding technique is used in the invention, so the length of microinstruction is reduced from 56 to 40 bits.

本发明采用精巧的编码,使微指令长度从56位缩短成40位。

No fragments were amplified from some loci of EST-SSRs, which may suggest the loss of these loci in mirror carp genome or sequence divergence at the primer binding sites. These null alleles may result from selection because functional genes are under more selection pressure than non-encoding loci. Overall, population genetic variation is high for each of the five mirror carp, and the differentiations are also significant among populations.

3在与功能基因相关的多个微卫星基因座位上,扩增产物呈现不同程度的缺失现象,这些无效等位基因的产生可能与结构基因在育种中受到人工选择的影响较大有关。

Digital Mapping the future of software development should be a non-point number, no e-plate encoding mirror site mapping system.

今后数字化测图软件的发展方向应该是一种无点号、无编码的镜站电子平板测图系统。

We could look at what the neural encoding is for this, says Mitchell.

我们可以看看这种情况神经解码是怎样的。

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Many will continue to choose to live in duality and in conflict.

许多人将继续选择活在二元对立性和冲突中。

I find that students of the University of Physical Education all wear sportswear at first sight.

我发现:体育大学的学生乍一看,都是穿运动衣,大家都一样

I love singing, but I don't want to take it as my lifelong career.

我喜欢唱歌,但我还不愿意把它当作我的终身职业。