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The transient transfection efficiency was measured by flow cytometry. The stable transfected cells were screened by G418. Results Electrotransfection had the highest transient transfection efficiency (56.8%), with the most masc clones of stably transfection and the least time taken to form big masc clones (20 days). Cationic polymer mediated transient transfection had the lowest efficiency (1.7%), with the least masc clones and the longest time taken to form the big masc clones (30 days). Cationic liposome transfection had a transient transfection efficiency of 39.9% and took 25 days to form the big masc clones.

结果 电转染有最高的瞬时转染效率(56.8%)，稳定转染得到的阳性克隆最多，且从细胞筛选到扩增至得到阳性大克隆所用的时间也最少（约20d）；用阳离子聚合物瞬时转染率低(1.7%)，稳定筛选同样可以得到阳性大克隆，但是数量很少且从细胞筛选到扩增至阳性大克隆所用的时间也最多（约30d）；阳离子脂质体转染效果居中，瞬时转染率为39.9%，从细胞筛选到扩增至得到阳性大克隆所用的时间约25d。

The result shows that JBD has different water absorbing-expanding properties in different medium, after absorbing it has very high strength and toughness, it also has good temperature resistant property and good efficiency of water plugging, with its temperature resistant capacity being higher than 120^0 and water plugging efficiency over 99% and oil plugging efficiency less than 15%.

但由于它吸水膨胀速度太快、吸水膨胀倍数过大、对地层的封堵强度较低、生产成本较高，使它在石油开发中的应用受到一定的局限。

And it comparatives and analyses the efficiency of three algorithm's cliping through an example, the example proved: Liang－Barsky algorithm efficiency is highest, Cohen-Sutherland next best, and the midpoint of division algorithm efficiency is lowest.

并通过一个实例对三种算法的裁剪效率比较分析，实例证明：梁友栋一Barsky算法效率最高，Cohen—Sutherland次之，而中点分割算法裁剪效率最低。

The braking efficiency is a main performance index in aircraft antiskid braking systems, the author studies the relationship between control accuracy and braking efficiency, so that we may design antiskid braking systems by means of braking efficiency directly, and this is the second creative point.

针对一种常用的描述结合系数和滑移率函数关系的表达式，研究了最佳滑移率、结合系数最大值和机轮抱死时的结合系数与该关系式中三个参数取值范围之间的联系，细化了这些参数的取值范围，减轻了应用迭代法求参数具体值的计算工作量。

The results show that the degassing efficiency increases with the residence time increasing;when other parameters are certain, the degassing efficiency increases with the number of arris and the concave depth increasing;the degassing efficiency decreases with the angle increasing.

结果表明：脱气效率随着滞留时间的增大而增大；当其他参数一定时，脱气效率随着棱数和凹入深度的增大而增大，随着角度的增大而减小。

In order to provide scientific basis and methods for study of walnut seedlings rapid propagation,survival ratio,scion's growth and efficiency of grafting were investigated and determined,efficiency of the new grafting method of walnut that it is single bud green branch grafting compared with efficiency of traditional grafting methods bud grafting and shield budding.

为给核桃优质苗木的快速繁育研究提供科学的依据和方法，就核桃嫁接的成活率、接穗的高生长、接穗的粗生长和嫁接的工作效率进行了调查和观测，比较分析了核桃嫁接新方法——单芽绿枝接与传统嫁接方法——"工"形芽接和嵌芽接的嫁接效果。

Comparing the synchronous rectifier to normal circuit by calculating the efficiency indicates that forestay is much better on efficiency and thermal performance. A simple circuit with good consistency, high efficiency, high reliability and low cost is presented in this paper. The feasibility is proved by emulating with computer.

通过分析已有的集中解决隔离型变换器输出带预偏置电压启动的解决方案，提出了一种全新的一致性好、成本低、效率高、简单和可靠性高的解决隔离变换器输出有预偏置电压的启动问题的电路，通过仿真验证了其可行性。

The article educes a relation about charcoal percent conversion and running temperature, gas caloricity, gasification efficiency, system efficiency by three programmes's calculation. So charcoal percent conversion has important influence on system's operation. And It is confirmed that air partial gasificat ion combined cycle generation system is a high efficiency, low pollution, techno logy simpleness, low investment, short periodicity generation system by comparis on with else combined cycle generation systems of different gasification mode.

文章通过对三种方案的计算，得出了碳的转化率与运行温度、煤气热值、气化效率、系统效率之间的关系，证实了碳的转化率对系统运行有重要的影响，同时和其他气化方式下联合循环发电系统进行比较分析，证实煤的空气部分气化联合循环系统是一高效低污染、技术简单、投资小、见效快的发电系统。

The article educes a relation about charcoal percent conversion and running temperature, gas caloricity, gasification efficiency, system efficiency by three programmes's calculation. So charcoal percent conversion has importantinfluence on system's operation. And It is confirmed that air partial gasificat ion combined cycle generation system is a high efficiency, low pollution, techno logy simpleness, low investment, short periodicity generation system by comparis on with else combined cycle generation systems of different gasification mode.

文章通过对三种方案的计算，得出了碳的转化率与运行温度、煤气热值、气化效率、系统效率之间的关系，证实了碳的转化率对系统运行有重要的影响，同时和其他气化方式下联合循环发电系统进行比较分析，证实煤的空气部分气化联合循环系统是一高效低污染、技术简单、投资小、见效快的发电系统。

3Photosynthetic efficiency was sensitive to the increase of plant density, which mainly caused by the decrease of content of chlorophyll, photosynthetic rate, and carboxylation efficiency which were positive correlation to yield per plant, and the decline of quanta efficiency was subordinate

3叶片光合效率对密度增加的反应较敏感，叶绿素含量、光合速率和羧化效率的降低是主要原因，它们与单株产量呈显著的正相关，叶绿素、光合速率和羧化效率之间也呈显著的正相关，量子效率的降低是次要的

Pachomius manual labour was organized as an essential part of the monastic life; and since it is a principle of the monks as distinguished from the mendicants, that the body shall be self-supporting, external work of one sort or another has been an inevitable part of the life ever since.

根据圣pachomius体力劳动组织作为一个重要组成部分寺院生活；以来，这是一个原则的和尚，作为有别于乞丐，该机构应自支撑，对外工作的这种或那种已一个必然的一部分，生活至今。

In order to study the effects of parametrical rolling on the ship movement and tilts, in allusion to ship's navigating in regular following waves, the equation of ship rolling motion is transformed into normal Mathieu equation by proper variable replacement when strong parametrical excitation in restoring moment varied according to sine or cosine wave.

为研究参数横摇对船舶运动和倾覆的影响，通过对船在规则波上随浪航行时，恢复力矩中出现的1个以正余弦规律变化的强参数激励项进行适当的变量置换，将船舶的横摇运动方程转换成标准形式的Mathieu方程。