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And then, the pBI101.2 wirelike vector and the promoter fragment of 886bp were acquired. Becase the promoter was modified by the HindⅢand BamH I, the acquired target genes fragment, digested by the restriction endonuclease, was matched to the wirelike vector.

由于在启动子两端有人工设计合成的HindⅢ和BamHⅠ酶切位点，经上述双酶切消化后，回收目的基因片段与线状载体的粘性接头恰好匹配。

Those carbohydrates not digested to monosaccharides by human enzymes in the small intestine may be metabolized by microorganisms in the large intestine, producing substances that are absorbed and catabolized for energy.

那些不能被人体小肠中的酶消化至单糖的会被大肠中微生物代谢，产生能被吸收和用于能量代谢的物质。

Those carbohydrates not digested to monosaccharides by human enzymes in the small intestine may be metabolized by microorganisms in the large intestine, producing substances that are absorbed and catabolized for energy.

无知的人开导更无知的人。7。那些不能被人体小肠中的酶消化至单糖的会被大肠中微生物代谢，产生能被吸收和用于能量代谢的物质。

Restriction endonuclease digested genomic DNA was circularized by self-ligation, and complete gene of α subunit and β subunit was obtained by inverse PCR.

以环化的限制性内切酶消化的基因组片段为模板，用反向PCR的方法扩增、克隆并测定了α亚基和β亚基的完整基因序列。

At the same time, the purified soil DNA was partially digested by enzyme Sau3AI, and the 2-6kb and 6-9kb fragments were recovered by dialyzing respectively.

纯化后的土壤DNA用限制性内切酶Sau3AI随机切割，以透析袋分别回收2-6kb和6-9kb的酶切片段，然后用T_4连接酶与上述脱磷载体连接。

The crude DNA pellets were recovered by dialyzing and then could be used for molecular operation such as amplification of 16S rRNA gene and digestion of restriction enzymes.The plasmid pUC19 was prepared and digested with BamHI.

粗提DNA用半透膜透析回收纯化，纯化后的DNA可以进行16sRNA基因PCR扩增，酶切等分子生物学操作，具有较高的质量。

They are suckled for two months, but at the end of that time they are able to eat half-digested flesh disgorged for them by their dam or even their sire.

他们是哺乳两个月，但在去年底，当时他们都能够吃半消化肉disgorged为他们自己的大坝或什至他们的妻妾。

Some books are to be tasted,others to be swallowed,and some few to be c hewed and digested.

一些书可以品味，另外一些书可以吞食，少数的一些可以咀嚼、消化。

Genomic DNA of maize was digested with restriction enzymes and hybridized with a mF2KP cDNA fragment.

应用不同的限制性内切酶消化玉米基因组DNA，以〓p标记的mF2KP cDNA片段作为探针进行Southern杂交分析。

Some key processes influencing construction of cotton bacterial artificial chromosome library, such as preparation of plug, DNA partial digestion, selection of digested fragment, molecular ratio between insert DNA andvector, concentration of ligates etc, were explored.

以我国抗病、优质、丰产的棉花优良品种中棉所12号为材料，对棉花细菌人工染色体文库构建过程中的一些关键技术，如plug的制备、DNA的部分消化、酶切片段的选择、插入片段与载体的摩尔比值、连接产物的浓缩等进行了研究，建立了构建棉花BAC文库的适宜方法体系。

Molecular Distillation is a kind of high vacuum distillation method, which is suitable for the separation of high boiling, heat sensitivity and viscidity products.

分子蒸馏技术是一种在高真空条件下进行的连续蒸馏过程，适合于分离高沸点、热敏性及具有生物活性的混合物。

They are to the Noughties what yuppies were to the Eighties .

那在六十年代是时髦的，但在八十年代肯定是过时了