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In this paper, PCR primers were designed based on the sequence of a single copy in atp6 gene. A 500bp fragment was amplified, and then the PCR products were digested with restriction enzym BglII.

根据atp6的基因序列设计特异扩增引物，以瓣化型胡萝卜核质互作雄性不育系H05A及其保持系H05B为试材，扩增获得了1条约500bp的特异扩增片段。

It has been reported that NDP kinase has weak interaction with Hsp70, which can increase the ATP hydrolysis activity, so we carefully examined the possibility of NDP kinase contamination in the experiment. The equimolar mixture of NDP kinase and Hsp70 were digested and then 44-kD fragment was purified.

为获得准确的结论排除NDP激酶可能对实验的影响，按照纯化Hsp70 44-kD片段的方法对等物质量NDP激酶和Hsp70混合物进行酶切、纯化，检测得到的44-kD片段的ATP-ADP转换活性，确定Hsp70 ATP-ADP转换活性是Hsp70自身固有的，位于其N端44-kD片段处。

Zinc deficiency in children's early performance was digested hypofunction.

小儿锌缺乏的早期表现是消化功能减退。

In vitro digestion methods for monogastric animal were reviewed accordi ng to simulatation of sites of the digestive tract,sources of digestive enzymes and the ways for separating digested and indigested nutrients in digesta.

本文从畜禽消化道模拟部位、消化酶的来源和分离已消化养分的方法等几方面对单胃动物体外消化模拟技术进行了归类和总结。

Methods:The subcutaneous adipose tissue was obtained from the fold inguen of four SD rats, then digested with collagenase type Ⅰ and cultured in endothelial medium for seven days.

获取4只雄性SD大鼠鼠蹊部皮下脂肪，通过机械分割和组织消化法获取脂肪基质细胞，在内皮细胞培养液中培养7d后，经免疫细胞化学及透射电镜鉴定证实95%以上细胞已具内皮细胞表型特征，再将这些细胞进行成脂(DMEM/F-12培养基内加入0.5mmol/L1-甲基-3-异丁基-黄嘌呤，1μmol/L地塞米松，10μmol/L胰岛素，200μmol/L吲哚美星)定向诱导7d，形态学观察和油红O特殊染色鉴定诱导后的细胞。

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建：设计引物，通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70／5UTR)、HSP70基因3&UTR序列(HSP70／3UTR)及β-微管蛋白基因内含子C序列，通过酶切连接，构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的，以β-微管蛋白基因内含子C序列作为间隔序列，以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70／5UTR-IntronC-HSP70／3UTR，序列测定结果正确。

Its value of BOD 5/COD fell to 0.19, moreover carbon and nitrogen was inversive, which brought much difficulty for anaerobically digested effluent to aerobically treatment.

猪场废水经过厌氧消化后，可生化性变差，BOD5/COD仅为0.19，并且碳、氮倒置，比例严重失调，给后续好氧处理带来很大困难。

Assisted by DNA2.0 and Gene2Oliga software, we optimized the codon usage and secondary structure of RNA and induced enzyme sites Cla I (237 site) and Pst I (475 site) into the gene. In the first step, fragments F1 (237 bp), F2 (238 bp) and F3 (422 bp) were separately synthesized by assembly PCR. In the second step, fragments F1, F2 and F3 were separately digested by Cla I and Pst I, and then ligated into a full length lipA gene.

首先在DNA2.0和Gene2Oliga软件辅助下对lipA基因密码子及RNA二级结构进行优化并引入Cla I(237位)和Pst I(475位)酶切位点；通过组装PCR分别合成lipA基因的各片段F1(237 bp)、F2(238 bp)和F3(422 bp)；通过该基因内的Cla I和Pst I限制性酶切位点连接成完整的全长lipA基因。

Schistosoma japonica antigen cDNA clones were identified by lysogenic expression,flat lyiic method and PCR amplification. All 8 positive clones immunologically screened could be expressed in E- coli in the form of fusion proteins with the molecular weight being about 140 to 150 kDa. The positive cDNA genes were digested by restriction endonuclease EcoRI,then the agarose gel electrophoresis revealed the size of them being 700 to 900 bp.

利用融源表达、平板裂解法和PCR扩增三种不同方法分别对日本血吸虫抗原cDNA基因进行鉴定和分析，8个免疫筛选阳性克隆均能在大肠杆菌中以融合蛋白的形式表达，表达蛋白分子量为140～150kDa，抗原cDNA基因经限制性内切酶EcoRI酶解后，琼脂糖凝胶电泳显示其大小为700～900bp，PCR能扩增出特异性条带。

Methods After the sample of Fenneropenaeus chinensis, Litopenaeus vannamei, Liza haematocheila, Acanthogobius hasta, Mactra veneriformis, Aloidis laevis, Ostrea denselamellosa and Fenneropenaeus chinensis was digested with concentrated hydrochloric acid, the lead content in marine products was measured by fluorimetric method and the results were compared with dithizone spectrophotometry.

采用湿式消解法对中国对虾、南美白对虾、梭鱼、矛尾刺虾虎鱼、四角蛤蜊、光滑兰蛤、密鳞牡蛎、中国对虾样品进行处理，采用浓盐酸作溶剂，荧光分光光度法直接检测铅含量，并与双硫腙光度法的测定结果进行比较。

Molecular Distillation is a kind of high vacuum distillation method, which is suitable for the separation of high boiling, heat sensitivity and viscidity products.

分子蒸馏技术是一种在高真空条件下进行的连续蒸馏过程，适合于分离高沸点、热敏性及具有生物活性的混合物。

They are to the Noughties what yuppies were to the Eighties .

那在六十年代是时髦的，但在八十年代肯定是过时了