查询词典 differentiate

The chondroblast cultured with compound porous materials PDLLA and PDLLA/chitosan. The aim are: 1 To examine the proliferation ability and potential chondroblast differentiation; 2 To find an ideal condition stimulated BMSC differentiate into chondroblast; 3 To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials; 4 To examine the release of cytokine in vitro.

目的在于：（1）通过骨髓基质干细胞的原代分离、培养及成软骨诱导，观察原代细胞扩增能力及其向成软骨方向分化的潜能；（2）寻求骨髓基质干细胞向成软骨方向分化的理想诱导条件；（3）观察经成软骨诱导的MSC在PDLLA、PDLLA／壳聚糖多孔载体上三维的增殖状况，探求细胞与材料间的相互作用；（4）观察经成软骨诱导的MSC在体外三维培养系统中细胞因子的释放状况，了解维持诱导细胞软骨表型的合适条件及作用机制。

MSC can be obtained by percoll fluid and density gradient centrifugation, this cells kept proliferation and differentiation ability in vitro; MSC can differentiate into chondroblast by lightly centrifugation and chemical definition medium, after one generation, the differential cells kept the phentype of chondroblast, they are a kind of ideal seed-cells for cartilage tissue engineering.

经percoll液密度梯度离心法能获得纯度较高的人骨髓间充质干细胞，这种细胞在体外培养体系中保持旺盛的增殖能力；经轻度离心和化学限定培养基法可诱导骨髓间充质干细胞向成软骨方向分化，增殖旺盛，可传一代培养，是软骨组织工程理想的种子细胞。

Now MSC become the chief seed-cell in cartilage tissue engineer, but the technology to stimulate MSC differentiate into chondroblast is not very successful,,the standard technology is culture the cell-ball in centrifuge tube,the reconstructured cartilage-like tissue is not achieve the mechanics of hyaline cartilage.

软骨组织工程的材料学研究主要体现在两类基质材料方面的进展，一类为天然基质材料，如胶原、壳聚糖、纤维素等；一类为人工合成可降解材料，如PGA、PLA等，但两者在来源、塑形能力及细胞识别信号等方面存在不足。

Part one Isolating, culturing, chondroblast induction and evaluation of human MSC in vitroObjective:To examine the property and proliferation ability of primary culturing MSC ,mvestgate the effect of lightly centrifugation and chemical definition medium to induce MSC differentiate into chondroblast.

结果：原代骨髓基质干细胞易于体外培养，增殖旺盛；经轻度离心和化学限定培养基诱导后细胞呈高密度生长，仍保持较旺盛的增殖能力，细胞转化成圆形肥大细胞，甲苯胺蓝染色显示软骨细胞外基质 v的特异性异染，11胶原蛋白免疫细胞化学染色呈强阳性，BT干CR鉴定显示＊胶原mRNA丰富的表达。

The aim are:lTo examine the proliferation ability and potential chondroblast differentiation ;2To find an ideal condition stimulated BMSC differentiate into chondroblast;3To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials;4To examine the release of cytokine in vitro.

取健康成人略后上棘处骨髓 5ml，经 percoll液分离后密度梯度离心，10'砌l密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心 5分钟，10V加 l接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质QCM）中的蛋白多糖，免疫细胞化学染色检测ECM中＊胶原的蛋白合成，RT干CR鉴定诱导细胞＊胶原mRNA的表达。

BMP-2 can induce BMSCs to differentiate into chondrocytes in monolayer culture in vitro. Cell passage is an important factor in the chondrogenesis differentiation of BMSCs.

结论BMP-2能诱导兔BMSCs在体外单层培养模式下向软骨细胞分化；细胞传代对BMSCs向软骨细胞分化有重要影响。

The conclusion is that this method is a good one to differentiate Lactic acid cocci.

通过本试验可得到以下结论，利用分析乳酸菌的16S rDNA的方法来鉴定乳酸球菌的可行性较好，准确性较高。

Of the research object and also to differentiate and analyze some easily confusable concepts,establishing some core concepts of this research, such as the surface meaning, deep meaning, double meanings etc, differentiating their differences and relations from literal meaning, implication, implicature, ambiguity.

确立了该研究中的一些核心概念，如语表意义、语内意义、双重意义等，分别辨析了其与字面意义、含意、言外之意、歧义的区别与联系。

That the internalized rules of behavior are social in origin does not differentiate this argument decisively from a utilitarian one, in which the source of utility functions is left open, leaving room for behavior guided entirely by consensually determined norms and values--as in the oversocialized view.

被内化的行为规则在被本质上是社会性的，这一点并不是与功利主义的观点完全对立的。在社会化不充分的讨论中，效益的功能是开放性的，它为解释由一致认同的规则和价值来全权指导的行为留有空间——就如同在过度社会化中的观点。

To differentiate the features of yin or yang is to deal with each concrete problem by making use of such basic contradictive relationship of the world.

辨别阴阳属性是在运用整个宇宙的最基本矛盾关系来解决每一个具体的问题。

The father that if work,mad father, love annoys, disposition is too indrawn the be short ofing that the father of Mu Ne, resign oneself to adversity causes father easily to love is broken; Communicate time effectively between father and son, Mu Zizhi to be less than a hour on average to wait a moment every week, it is the cause that causes the child to become addiction.

如工作狂的父亲、爱愤怒的父亲、性格过于内向木讷、逆来顺受的父亲都易造成父爱的缺失；父子、母子之间的有效沟通时间每周平均小于一小时等等，都是造成孩子成瘾的原因。

These are vandal proof and they come with a locking mechanism.

这是野蛮的证据，他们来到了锁机制。