查询词典 cultured

ABSTRACT Penaeus monodon and Litopenaeus vannamei are cultured penaeid shrimp all over the world, and they are the major cultured shrimp in China.

由白斑综合症病毒引起的白斑综合症是斑节对虾和凡纳滨对虾最主要病害之一。

Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of MCSF (25μg/L) with different concentrations of TNF-α(0, 1, 10, 100 μg/L) for 5 days, the formation of TRAP multinucleated cells was observed. These cells were also cultured in the presence of both RANKL (30 μg/L) and M-CSF (25 μg/L) with or without 10 μg/L TNF-α for4, 5, 6 and 9 days. The number of TRAP multinucleated cells and resorption pits on dentine slices were counted under light microscope.

选用小鼠巨噬细胞集落刺激因子依赖性非附着性骨髓细胞，在含有25μg/L M-CSF和0，1，10，100μg/L TNF-α的α-MEM培养液中培养5d后，观察抗酒石酸酸性磷酸酶染色阳性多核细胞的形成；细胞在含有25μg/L M-CSF和30μg/L sRANKL的α-MEM培养液中进行培养，比较加入和不加入10μg/L TNF-α培养4、5、6和9d后，所形成的TRAP多核细胞的数目和骨吸收面积。

AIM: To investigate the effect of enhanced green fluorescence protein gene transfection on the cell cycle distribution of primary cultured human chondrocytes in order to establish a tracking method of cultured human nasoseptal chondrocytes.

目的 ：观察转染增强型绿色荧光蛋白基因后原代培养的人鼻中隔软骨细胞的细胞周期的变化，建立原代培养的人鼻中隔软骨细胞的示踪方法。

Primary cultured human chondrocytes,which were initially obtained from the nasoseptal cartilage, were cultured in vitro and transferred with pEGFP-N1 by means of electroporation wi...

结果 ：增强型绿色荧光蛋白基因在转染 2 4h后得到了明显表达，4 8h后流式细胞仪检测其表达率为 35 37%，细胞周期没有明显的改变，且未影响软骨细胞的贴壁过程。

METHODS: mononuclear cells were isolated from axillary draining lymph node, and the adherent cells were cultured with rhGMCSF and rhIL4 to induce DC. The nonadherent cells were cultured with IL2 to induce tumor draining lymph node cells.

以多种细胞因子联合诱导腋下淋巴结单个核细胞中的贴壁细胞为DC，非贴壁细胞与IL2共培养后诱导成为肿瘤区域引流淋巴结细胞，用自体乳腺癌细胞冻融抗原刺激DC，并和TDLNC共培养，以诱导肿瘤抗原特异性CTL。

METHODS: The mononuclear cells were isolated from axillary draining lymph node, and the adherent cells were cultured with rhGMCSF and rhIL4 to induce DC. The nonadherent cells were cultured with IL2 to induce tumor draining lymph node cells.

以多种细胞因子联合诱导腋下淋巴结单个核细胞中的贴壁细胞为DC，非贴壁细胞与IL2共培养后诱导成为肿瘤区域引流淋巴结细胞，用自体乳腺癌细胞冻融抗原刺激DC，并和TDLNC共培养，以诱导肿瘤抗原特异性CTL。

Results Cells cultured in serum-free medium AIMV could proliferate,kill target cellsand express CD11 c well enough to substitute the cells cultured in standard serum-containing medium.Proliferation,cytotoxicity and expression of CD11 c of A-NK were better than nonadherent natural killer cells.Death patterns of tumor target cells were necrosis and apoptosis.

结果 与完全培养基培养细胞相比，AIMV培养细胞增殖能力、杀伤肿瘤细胞的能力、表达CD11 c 的能力与之相当；在同一培养条件下A-NK细胞的增殖能力、杀伤活性及表达CD11 c 的能力明显强于NA-NK细胞；被A-NK细胞杀伤的肿瘤细胞死亡形式是溶解坏死和凋亡。

Results Cells cultured in serum-free medium AIMV could proliferate,kill target cellsand express CD11 c well enough to substitute the cells cultured in standard serum-containing medium.Proliferation,cytotoxicity and expression of CD11 c of A-NK were better than nonadherent natural killer cells.Death patterns of tumor target cells were necrosis and apoptosis.

结果 与完全培养基培养细胞相比，AIMV培养细胞增殖能力、杀伤肿瘤细胞的能力、表达CD11 c 的能力与之相当；在同培养条件下A-NK细胞的增殖能力、杀伤活性及表达CD11 c 的能力明显强于NA-NK细胞；被A-NK细胞杀伤的肿瘤细胞死亡形式是溶解坏死和凋亡。

CD44~+CD24~ cells in MCF-7 increased after cultured in serum-free medium. The cancer stem-like cells in MCF-7 grew as clonal nonadherent spherical clusters. The percentage of CD44~+CD24~ cells reached up to 29.23% after cultured 3 weeks in serum-free medium.

结论SP检测与CD44~+CD24~细胞检测是目前乳腺癌干细胞检测的两种常用方法，但两者用于同一种乳腺癌细胞系中的检测结果并不一致，均有一定的局限性。

Skin keratinocytes and epithelial cells from hair follicle organized into epidermoid cyst-like spheroids when cultured on nubby dermal papilla cells gel and epidermoid layer-like structure was formed when they were cultured on free dermal papilla cells and skin fibroblasts gel.

团块状的毛乳头细胞诱导毛囊上皮细胞和皮肤角质形成细胞形成球形结构；而游离分散的毛乳头细胞和皮肤成纤维细胞诱导毛囊上皮细胞和皮肤角质形成细胞形成表皮样层化结构。

Means the parent directory, so this command means to execute "toolchain.sh," which is in the current directory.

代表父目录，所以这个命令就是执行当前目录下的"toolchain.sh"。

Yes,In fact,I'm on our city ream.

是的。事实上我是我们市队的。