查询词典 cultured

The present study is to culture otocyst cells in fetal, cochlear sensory epithelial cells of neonatal and mature respectively in vitro with refinement of culture media and techniques. Immunocytochemistry and Bromodeoxyuridine labeling are used to detect properties and mitotic status of cultured cells. With the observations about proliferation and differentiation in cultured cells, we want to find the origin of regenerative cells and to understand how these progenitors proliferate and differentiate.

本研究分别对胎鼠听囊、新生鼠听觉上皮和成年鼠听觉上皮用自然培养方法进行体外培养，并应用免疫细胞化学方法，对培养细胞进行染色标记和观察，系统了解哺乳动物听觉细胞发生、发育过程，以及这些细胞在体外的增殖和分化过程，旨在了解哺乳动物听觉细胞听觉上皮感觉细胞的来源，发现细胞生长的前体细胞。

Methods: The health adult human primary hepatocyte microsome was randomly divided into 12 groups for control and itraconazole with different concentrations in the range of clinical drug blood concentration, each group have 10 samples. Control groups were added culture fluid, itraconazole groups were added itraconazole with different concentration respectively, after cultured 30 minutes, substrates (phenacetin for CYP1A2, testosterone for CYP3A4) were added and cultured for another 40 minutes.

采用健康成人肝细胞微粒体，分为对照组和不同浓度的伊曲康唑组，共12组，每组10个样本，伊曲康唑组分别加入血药浓度范围内不同对应浓度的伊曲康唑，对照组仅加入培养液，孵育30 min后，再加入CYP450同工酶1A2和3A4的相应底物再孵育40 min。

Times as that of T. sp-1 cultured under phototrophic conditions and 3. 2 times as that of T. sp-1 cultured under heterotrophic conditions.

在兼养条件下，T.sp-1的生长速率达0.222 g／，分别为自养和异养培养条件下的1.5倍和3.2倍。

When it comes to fatty acid compositions, PUFAs contents of T. sp-1 cultured in the mixotrophic and phototrophic conditions are 55. 44% and 44. 20% of total fatty acids, respectively. The contents of saturated and monosaturated fatty acids of T. sp-1 cultured under heterotrophic conditions are higher, their contens are 32. 84% and 35. 29% of total fatty acids, respectively.

在脂肪酸组成方面，T.sp-1在兼养和自养条件下培养，脂肪酸以PUFAs为主，含量分别占总脂肪酸的55.44％和44.20％；而在异养条件下培养，脂肪酸以饱和脂肪酸和单饱和脂肪酸为主，含量分别占总脂肪酸的32.84％和35.29％。

Compared with the cell cultured in BG11, the cell wall of the cells cultured at low concentration of Cu2+(0.01～1 mg/L) did not markedly become thick and the chlorophyll did not change notably, but the number of pyrenoid increased.

结果表明，Cu2+对绿球藻的显微结构﹑生长及生理状态的影响比较显著。

METHODS: In the experimental group, ADSCs were cultured with the scaffold and induced by CDMP1(50 μg/L) in basic medium for another 2 weeks. In the control group, SD rat cartilage cells were cultured with elementary nutrient liquid with scaffold for another 2 weeks. Twenty nude mice were imbedded the composite of cells-scaffold of the experimental group in their left armpits and the composites of cells-scaffold of the control group in right armpits. Every ten nude mice were killed at 8 and 12 weeks and were examined by safranine O-fast green and toluidine blue staining.

体外培养的脂肪干细胞复合于支架上，加入诱导液(基础培养液+ 50 μg/L软骨形态发生蛋白1)继续培养2周作为实验组；将SD仔鼠软骨细胞复合于支架上，常规培养2周作为对照组。20只裸小鼠，左侧腋窝皮下均植入实验组细胞-支架复合物，右侧腋窝皮下均植入对照组细胞-支架复合物。8， 12周各处死10只，行甲苯胺蓝和番红O-固绿染色。

Primary cultured rat hepatocytes were cultured with various concentrations of sodium fluoride for 24 hours to study the toxicity and possible mechanism induced by fluoride from morphological, enzymological and biochemical changes.

收集细胞培养上清液，测定ALT和AST活性和MDA含量。用上述指标来评价氟化钠对肝细胞的毒性作用及其可能机制。

In this report, immunohistochemistry and in situ hybridization techniques were perfected to examine the expression of bFGF, fibroblast growth factor receptor-I and bFGFmRNA of cultured HEP-2 laryngo-tumor cell and 803 gastro-tumor cell and of the solid tumor tissue formed by implanting the cultured cells into subcutis of nude mice, intending to elucidate the correlation between the expression level and the tumor formation.

本研究建立了免疫组化和原位杂交的检测方法，通过对体外培养的HEP-2喉癌细胞和803胃癌细胞和这两株细胞在裸鼠皮下形成的实体瘤组织中bFGF，纤维细胞生长因子受体-1（fibroblast growth factor receptor-1，FGFR-1）和bFGFmRNA的检测试图揭示三者在体外和体内表达以及与肿瘤形成的关系，从而为这方面的研究提供更多的材料。

The experiments showed a significant increase in Bax mRNA levels (P.01) in neurons cultured at severe hypoxia condition compared with those cultured at normoxia. In hypoxia groups, tolbutamide increased Bax mRNA expression (P.05), while diazoxide reduced it (n=5 for each).

缺氧+二氮嗪组中基因Bax的mRNA表达水平与单纯缺氧组相比下降(P.01)，缺氧＋甲苯磺丁脲组中基因Bax的mRNA表达水平相对于单纯缺氧组也有提高(P.05)。

RESULTS: The AF cells cultured in different substrates were morphologically undistinguishable. Toluidine blue staining showed that there was also no difference between AF cells cultured on flexible silicone membranes and in plastic plates. The cells growing in different substrates expressed the same levels of collagen typeⅠ,Ⅱ and aggrecan mRNA and integrin β1. AF cells grew well on silicone membranes.

结果：种植于不同基质的纤维环细胞形态学观察无明显差别；在两种基质上的纤维环细胞甲苯胺蓝染色无差异；Ⅰ，Ⅱ型胶原及聚集蛋白聚糖mRNA及细胞膜整合素β1的表达亦无明显差异；纤维环细胞可以黏附于硅橡胶膜上生长。

Means the parent directory, so this command means to execute "toolchain.sh," which is in the current directory.

代表父目录，所以这个命令就是执行当前目录下的"toolchain.sh"。

Yes,In fact,I'm on our city ream.

是的。事实上我是我们市队的。