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METHODS: Human bronchial epithelium cells were isolated and cultured from 18 patients undergone pueumonectomy in hospital. Interleukin 4 or interleukin 6 at concentration of 10 μg/L was used to treat these cultured cells, and Iamil was measured by whole cell patch clamp techniques.

体外分离培养气道上皮细胞，经过终浓度均为10 μg/L的IL-4、IL-6处理，用全细胞膜片钳技术记录原代培养的气道上皮细胞中Iamil。

METHODS: Stably transfected cells were cultured per well in a 6well tissue culture plate and cultured for 24 h.

将稳定转染的HeLaNS5A和HeLaNS5AΔISDR细胞在6孔培养板中培养24 h后，加入人基因工程α2a型干扰素(rHuIFNα2a)至所需浓度。

The test groups were drenched orally with different dosages of compound Radix scutellariae granules. After being drenched orally for ten days, their thymus-index and spleen-nidex, immune globulins of IgA, IgG, IgM and alexins of C3, C4 of serum were defermined, and experiments in vivo were done. The mice-spleen's lymphocytes which were cultured in vivo were added with different concentrations of compound Radix scutellariae granules. The lymphocytes translation stimulate indexes were measured. The ability of licking up neutral red of the peritoneal macrophages cultured in vivo was measured in the groups.

应用复方黄芩颗粒给小鼠灌胃10d后，分别测定对照组与实验组胸腺指数、脾脏指数和血清中的免疫球蛋白IgA、IgG、IgM和补体C3、C4；并且进行体外实验，用不同浓度的复方黄芩颗粒剂对小鼠脾脏淋巴细胞进行体外培养，测定淋巴细胞转移刺激指数；在体外培养测定细胞吞噬中性红的能力。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明，所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达；在缺乏AMBN情况下，与对照组相比，实验组牙胚在体外可以继续生长发育至钟状晚期，出现成釉细胞和成牙本质细胞的分化，成釉细胞可以分化成为分泌期型成釉细胞，胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体，缺乏溶酶体，表明对蛋白合成和脚的能力降低；实验组牙胚有牙尖形成和基质分泌，但牙尖形态异常，基质形成减少，牙尖周围基质最厚处为O.6卜m，明显薄于对照组的5.spin，基质中胶原纤维粗细不等，排列稀疏， 3 第四军医大学硕士学位论文未见钙化现象，充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程，影响胶原纤维和牙本质基质的合成，促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

Methods Macrophagea were cultured in 6-hole plate and coverslip was prepared. Then macropages were cultured one day with different concentrations(0, 12.5, 25.50, 100, 200 mg/L) of matrine, oxymatrine and sophordine; the expression of CD91 and CD13 by immunohistochemical dyeing was analyzed by Image-Pro plus system (Media Cybemetic, Inc., 6.0 edition).

(1)用6孔板培养巨噬细胞RAW264.7，制作细胞爬片，然后与不同浓度(0、12.5、25、50、100、200 mg/L)的苦参碱、氧化苦参碱和槐定碱共培养，细胞爬片免疫组化染色，检测CD91和CD13的表达情况，并采用Media Cybernetic、公司的图像分析系统进行定量分析，测量积分光密度值。

Enucleated within 24 hours after death. 2. immunohistochemical identification of cultured conjunctival cells When the second cultured generation of conjunctival cells close to confluence, the glass coverslips were removed in situ fixation by methanol and DAB color immunohistochemical staining by CK13 monoclonal antibody in time.

由于其具有多向分化的潜能，在体外又较容易培养扩增，加之具有潜在的细胞治疗价值，故成为生物学界和医学界的研究热点。

Cultured PC12 cells were incubated with Fluo 3-AM working solution (the final concentration of Fluo 3-AM is 5μmol/L) at 37℃for 45min, followed by 3 times washes with the culture medium. The cultured neurons grown on glass coverslips were placed in a cell chamber on the stage of an inverted microscope.

多种应激刺激通过细胞内信号转导途径使CREB第133位的丝氨酸磷酸化而激活，活化的CREB直接或间接激活相关基因的转录，进而表述某些蛋白分子以及某些神经营养因子等，进而调节神经元在应激性损伤后再生、存活及修复等。

Immunohistochemical identification of cultured conjunctival cellsWhen the second cultured generation of conjunctival cells close to confluence, the glass coverslips were removed in situ fixation by methanol and DAB color immunohistochemical staining by CK13 monoclonal antibody in time.3. growth curve of conjunctival cellsThe 1st,3rd,5th passage cells were selectecd randomly and subcultured at the density of 5×10~4/ml,after culturing for another 1-9 days, were counted and compared the cell number every day.

采用消化后组织块法培养结膜原代细胞，并进行传代。2、培养结膜细胞的免疫组化鉴定把第2代的结膜细胞接种于置在培养皿中的盖玻片上，当细胞接近融合时，及时取出作原位甲醇固定，CK13单抗、DAB显色免疫组化染色。3、结膜细胞生长曲线的测定随机选取第1、3、5传代细胞，以5×10~4／ml密度传代、分别培养1-9天，每天分别细胞计数并进行分析比较，重复3次。

The effect of gentamicin on survival and neuritogenesis of cultured spiral ganglion cell from NIH mouse 1-5 days after birth was observed.Different doses (25,50 and 100 mg/L)of gentamicin were add to the medium of SGC,and cultured for 14 days.

为观察硫酸庆大霉素对培养的小鼠耳蜗螺旋神经节细胞的细胞生存数和细胞突起长度的影响，在单离培养的出生1～5 d的NIH小鼠SGC培养液中，加入不同浓度的硫酸庆大霉素(25，50，100 mg/L)。

Results TLC can be used for the identification in-vitro cultured Calculus Bovis and artificial Calulus Bovis, the samples containing cultured Calulus Bovis showed the specific spots of bilirubin but no hyodeoxycholic acid spot while the samples containing artifical Calulus Bovis showed the hyodeoxycholic acid spot but no bilirubin spot.

含人工牛黄的样品有猪去氧胆酸斑点，含体外培育牛黄的样品未检出有与猪去氧胆酸对照品相对应的斑点；含人工牛黄的样品不显胆红素斑点，含体外培育牛黄的样品显示与胆红素对照品相对应的斑点。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。