查询词典 control assay

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

PART 1 UNIT 1 B Electrical and Electronic Engineering Basics A Electrical Networks ———————————— 3 Three-phase Circuits A The Operational Amplifier ——————————— 5 UNIT 2 B Transistors A Logical Variables and Flip-flop —————————— 8 UNIT 3 B Binary Number System A Power Semiconductor Devices —————————— 11 UNIT 4 B Power Electronic Converters A Types of DC Motors —————————————15 UNIT 5 B Closed-loop Control of DC Drivers A AC Machines ———————————————19 UNIT 6 B Induction Motor Drive A Electric Power System ————————————22 UNIT 7 B PART 2 UNIT 1 B Power System Automation Control Theory A The World of Control ————————————27 —————29 The Transfer Function and the Laplace Transformation UNIT 2 B A Stability and the Time Response ————————— 30 Steady State————————————————— 31 A The Root Locus ————————————— 32 ————— 33 UNIT 3 B The Frequency Response Methods: Nyquist Diagrams UNIT 4 A The Frequency Response Methods: Bode Piots ————— 34 B Nonlinear Control System 37 UNIT 5 A Introduction to Modern Control Theory B State Equations 40 38 UNIT 6 A Controllability, Observability, and Stability B Optimum Control Systems UNIT 7 A Conventional and Intelligent Control B Artificial Neural Network Computer Control Technology A Computer Structure and Function 42 B Fundamentals of Computer and Networks 43 44 PART 3 UNIT 1 UNIT 2 A Interfaces to External Signals and Devices B The Applications of Computers 46 UNIT 3 A PLC Overview B PACs for Industrial Control, the Future of Control UNIT 4 A Fundamentals of Single-chip Microcomputer 49 B Understanding DSP and Its Uses 1 UNIT 5 A A First Look at Embedded Systems B Embedded Systems Design Process Control A A Process Control System B 50 PART 4 UNIT 1 Fundamentals of Process Control 52 53 UNIT 2 A Sensors and Transmitters B Final Control Elements and Controllers UNIT 3 A P Controllers and PI Controllers B PID Controllers and Other Controllers UNIT 4 A Indicating Instruments B Control Panels Control Based on Network and Information A Automation Networking Application Areas B Evolution of Control System Architecture PART 5 UNIT 1 UNIT 2 A Fundamental Issues in Networked Control Systems B Stability of NCSs with Network-induced Delay UNIT 3 A Fundamentals of the Database System B Virtual Manufacturing—A Growing Trend in Automation UNIT 4 A Concepts of Computer Integrated Manufacturing B Enterprise Resources Planning and Beyond Synthetic Applications of Automatic Technology A Recent Advances and Future Trends in Electrical Machine Drivers B System Evolution in Intelligent Buildings PART 6 UNIT 1 UNIT 2 A Industrial Robot B A General Introduction to Pattern Recognition UNIT 3 A Renewable Energy B Electric Vehicles UNIT 1 A

电路 2 电路或电网络由以某种方式连接的电阻器，电感器和电容器等元件组成。如果网络不包含能源，如电池或发电机，那么就被称作无源网络。换句话说，如果存在一个或多个能源，那么组合的结果为有源网络。在研究电网络的特性时，我们感兴趣的是确定电路中的电压和电流。因为网络由无源电路元件组成，所以必须首先定义这些元件的电特性。就电阻来说，电压-电流的关系由欧姆定律给出，欧姆定律指出：电阻两端的电压等于电阻上流过的电流乘以电阻值。在数学上表达为： u=iR (1-1A-1)式中 u=电压，伏特；i =电流，安培；R =电阻，欧姆。纯电感电压由法拉第定律定义，法拉第定律指出：电感两端的电压正比于流过电感的电流随时间的变化率。因此可得到：U=Ldi/dt 式中 di/dt =电流变化率，安培/秒； L =感应系数，享利。电容两端建立的电压正比于电容两极板上积累的电荷 q 。因为电荷的积累可表示为电荷增量 dq 的和或积分，因此得到的等式为 u=，式中电容量 C 是与电压和电荷相关的比例常数。由定义可知，电流等于电荷随时间的变化率，可表示为 i = dq/dt。因此电荷增量 dq 等于电流乘以相应的时间增量，或 dq = i dt，那么等式(1-1A-3)可写为式中 C =电容量，法拉。

PART 1 Electrical and Electronic Engineering Basics UNIT 1 A Electrical Networks B Three-phase Circuits UNIT 2 A The Operational Amplifier ——————————— 5 B Transistors UNIT 3 A Logical Variables and Flip-flop —————————— 8 ———————————— 3 B Binary Number System UNIT 4 A Power Semiconductor Devices —————————— 11 B Power Electronic Converters UNIT 5 A Types of DC Motors —————————————15 B Closed-loop Control of DC Drivers UNIT 6 A AC Machines ———————————————19 B Induction Motor Drive UNIT 7 A Electric Power System ————————————22 B Power System Automation PART 2 Control Theory UNIT 1 A The World of Control ————————————27 B The Transfer Function and the Laplace Transformation UNIT 2 A B —————29 Stability and the Time Response ————————— 30 ————————————— 32 Steady State————————————————— 31 UNIT 3 A The Root Locus B The Frequency Response Methods: Nyquist Diagrams ————— 33 UNIT 4 A The Frequency Response Methods: Bode Piots ————— 34 B Nonlinear Control System 37 UNIT 5 A Introduction to Modern Control Theory B UNIT 6 State Equations 40 38 A Controllability, Observability, and Stability B Optimum Control Systems UNIT 7 A Conventional and Intelligent Control B Artificial Neural Network PART 3 UNIT 1 Computer Control Technology A Computer Structure and Function B 42 43 44 Fundamentals of Computer and Networks UNIT 2 A Interfaces to External Signals and Devices B The Applications of Computers 46 UNIT 3 A PLC Overview B PACs for Industrial Control, the Future of Control 1 UNIT 4 A Fundamentals of Single-chip Microcomputer B Understanding DSP and Its Uses 49 UNIT 5 A A First Look at Embedded Systems B Embedded Systems Design PART 4 UNIT 1 Process Control A A Process Control System 50 B Fundamentals of Process Control 53 52 UNIT 2 A Sensors and Transmitters B Final Control Elements and Controllers UNIT 3 A P Controllers and PI Controllers B PID Controllers and Other Controllers UNIT 4 A Indicating Instruments B Control Panels PART 5 UNIT 1 Control Based on Network and Information A Automation Networking Application Areas B Evolution of Control System Architecture UNIT 2 A Fundamental Issues in Networked Control Systems B Stability of NCSs with Network-induced Delay UNIT 3 A Fundamentals of the Database System B Virtual Manufacturing—A Growing Trend in Automation UNIT 4 A Concepts of Computer Integrated Manufacturing B Enterprise Resources Planning and Beyond PART 6 UNIT 1 Synthetic Applications of Automatic Technology A Recent Advances and Future Trends in Electrical Machine Drivers B System Evolution in Intelligent Buildings UNIT 2 A Industrial Robot B A General Introduction to Pattern Recognition UNIT 3 A Renewable Energy B Electric Vehicles 2 UNIT 1 A

电路 电路或电网络由以某种方式连接的电阻器，电感器和电容器等元件组成。如果网络不包含能源，如电池或发电机，那么就被称作无源网络。换句话说，如果存在一个或多个能源，那么组合的结果为有源网络。在研究电网络的特性时，我们感兴趣的是确定电路中的电压和电流。因为网络由无源电路元件组成，所以必须首先定义这些元件的电特性。就电阻来说，电压-电流的关系由欧姆定律给出，欧姆定律指出：电阻两端的电压等于电阻上流过的电流乘以电阻值。在数学上表达为： u=iR (1-1A-1)式中 u=电压，伏特；i =电流，安培；R =电阻，欧姆。纯电感电压由法拉第定律定义，法拉第定律指出：电感两端的电压正比于流过电感的电流随时间的变化率。因此可得到：U=Ldi/dt 式中 di/dt =电流变化率，安培/秒； L =感应系数，享利。电容两端建立的电压正比于电容两极板上积累的电荷 q 。因为电荷的积累可表示为电荷增量 dq 的和或积分，因此得到的等式为 u=，式中电容量 C 是与电压和电荷相关的比例常数。由定义可知，电流等于电荷随时间的变化率，可表示为 i = dq/dt。因此电荷增量 dq 等于电流乘以相应的时间增量，或 dq = i dt，那么等式(1-1A-3)可写为式中 C =电容量，法拉。

PART 1 Electrical and Electronic Engineering Basics UNIT 1 A UNIT 2 A UNIT 3 A UNIT 4 A UNIT 5 A UNIT 6 A UNIT 7 A Electrical Networks ———————————— 3 B Three-phase Circuits The Operational Amplifier ——————————— 5 Logical Variables and Flip-flop —————————— 8 Power Semiconductor Devices —————————— 11 Types of DC Motors —————————————15 AC Machines ———————————————19 Electric Power System ————————————22 B Transistors B Binary Number System B Power Electronic Converters B Closed-loop Control of DC Drivers B Induction Motor Drive B Power System Automation PART 2 Control Theory UNIT 1 A B UNIT 2 A UNIT 3 A UNIT 4 A The World of Control ————————————27 Stability and the Time Response ————————— 30 The Root Locus ————————————— 32 The Transfer Function and the Laplace Transformation —————29 B Steady State————————————————— 31 B The Frequency Response Methods: Nyquist Diagrams ————— 33 The Frequency Response Methods: Bode Piots ————— 34 B Nonlinear Control System 37 UNIT 5 A Introduction to Modern Control Theory B B B PART 3 B B B State Equations Optimum Control Systems Artificial Neural Network Computer Control Technology 42 43 44 Fundamentals of Computer and Networks The Applications of Computers 46 40 38 UNIT 6 A Controllability, Observability, and Stability UNIT 7 A Conventional and Intelligent Control UNIT 1 A Computer Structure and Function UNIT 2 A Interfaces to External Signals and Devices UNIT 3 A PLC Overview PACs for Industrial Control, the Future of Control 1 UNIT 4 A Fundamentals of Single-chip Microcomputer 49 B B PART 4 B B B B PART 5 B B B B PART 6 Understanding DSP and Its Uses Embedded Systems Design Process Control 50 52 53 Fundamentals of Process Control UNIT 5 A A First Look at Embedded Systems UNIT 1 A A Process Control System UNIT 2 A Sensors and Transmitters Final Control Elements and Controllers PID Controllers and Other Controllers Control Panels Control Based on Network and Information Evolution of Control System Architecture Stability of NCSs with Network-induced Delay Virtual Manufacturing—A Growing Trend in Automation Enterprise Resources Planning and Beyond Synthetic Applications of Automatic Technology UNIT 3 A P Controllers and PI Controllers UNIT 4 A Indicating Instruments UNIT 1 A Automation Networking Application Areas UNIT 2 A Fundamental Issues in Networked Control Systems UNIT 3 A Fundamentals of the Database System UNIT 4 A Concepts of Computer Integrated Manufacturing UNIT 1 A Recent Advances and Future Trends in Electrical Machine Drivers B B B System Evolution in Intelligent Buildings A General Introduction to Pattern Recognition Electric Vehicles UNIT 2 A Industrial Robot UNIT 3 A Renewable Energy 2 UNIT 1 A

电路 电路或电网络由以某种方式连接的电阻器，电感器和电容器等元件组成。如果网络不包含能源，如电池或发电机，那么就被称作无源网络。换句话说，如果存在一个或多个能源，那么组合的结果为有源网络。在研究电网络的特性时，我们感兴趣的是确定电路中的电压和电流。因为网络由无源电路元件组成，所以必须首先定义这些元件的电特性。就电阻来说，电压-电流的关系由欧姆定律给出，欧姆定律指出：电阻两端的电压等于电阻上流过的电流乘以电阻值。在数学上表达为： u=iR (1-1A-1)式中 u=电压，伏特；i =电流，安培；R =电阻，欧姆。纯电感电压由法拉第定律定义，法拉第定律指出：电感两端的电压正比于流过电感的电流随时间的变化率。因此可得到：U=Ldi/dt 式中 di/dt =电流变化率，安培/秒； L =感应系数，享利。电容两端建立的电压正比于电容两极板上积累的电荷 q 。因为电荷的积累可表示为电荷增量 dq 的和或积分，因此得到的等式为 u=，式中电容量 C 是与电压和电荷相关的比例常数。由定义可知，电流等于电荷随时间的变化率，可表示为 i = dq/dt。因此电荷增量 dq 等于电流乘以相应的时间增量，或 dq = i dt，那么等式(1-1A-3)可写为式中 C =电容量，法拉。

Methods By using Vero cell as the host cells and with acyclovir as the positive control, anti-HSV-1 activity of the extract was observed and determined with cytopathic effect assay and plaque reduction assay. Initial studies on antiviral mechanism included three aspects: virucidal assay, attachment assay and penetration assay.

以Vero细胞为宿主细胞，阿昔洛韦为阳性对照药物，通过观察细胞病变效应与空斑减数实验测定川楝子提取物抗HSV-1活性，计算其IC50与治疗指数，并从药物对病毒的直接灭活作用、对病毒吸附的影响及对病毒穿膜的影响三个方面初探川楝子提取物抗HSV-1活性的机理。

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

objective to study the efficacy of the tr/patoc 1 slide agglutination assay for vaccination new pentavalent whole cell vaccine for leptospirosis.methods using tr/patoc 1 slide agglutination assay to detect the searial of serum specimens of 100 healthy people after vaccination for leptospirosis and comparing with the microsopic agglutination test.results both basic immunizaton and 20 days after the enhanced immunization,their positive seroconversion rates were higher than 90% in tr/patoc 1 slide agglutination assay,they were not signficantly different between slide agglutination assay and mat,the antibody titer was greatly lower in 90 days after basic immunization.conclusion the tr/patoc 1 slide agglutionation assay was one of useful assays in evaluation the lately immune efficency of leptospira vaccination.

目的 采用tr/patoc 1玻片凝集试验评价钩端螺旋体疫苗免疫效果及应有价值。方法采用tr/patoc 1玻片凝集试验检测100名健康人钩端螺旋体疫苗免疫后的血清抗体变化，并与显微镜凝集试验比较。结果无论是基础免疫还是加强免疫20？d后，玻凝试验抗体阳性率高达90%以上，与标准的显微镜凝集试验比较差异无统计学意义，玻片凝集试验检测的抗体持续时间较短，90？d后阳性率已大幅度降低，表明sat检测的抗体是早期抗体。结论 tr/patoc 1玻片凝集试验操作简易，可以作为基层卫生机构监测钩端螺旋体疫苗近期免疫效果的方法之一。

After mass chemotherapy,ScAg assay in case screen for selective chemotherapy was better than that of ScAb assay and stool examination.ScAg assay was also practice in evaluation of control effect,disease surveillance in children,and to confirm repeat treatment cases.In short,ScAg assay applied for case screen would play an important role in control program.

结论检测ScAg用于全民化疗后选择性化疗的筛查方法优于ScAb及粪便检查，适用于防治效果考核，儿童感染的监测及确定重复治疗对象，经多年应用ScAg筛查血吸虫病对达到传播控制起到重要作用。

Unit 22 400vac low voltage distribution pen busbar unit 22 400vac low voltage distribution cubicle lighting.plug and heating unit 22 400vac low voltage distribution measuring and ac-distribution unit 22 400vac low voltage distribution 220vdc control2 unit 22 400vac low voltage distribution control circuit breaker unit 22 400vac low voltage distribution automatic interconnection2 unit 22 device list unit 22 400vac low voltage distribution cubicle lighting.plug anh heating unit 22 400vac low voltage distribution ac/dc supply control unit 22 400vac low voltage distribution cb supply 1 control unit 22 400vac low voltage distribution cb coupling control unit 22 400vac low voltage distribution cb supply 2 control unit 22 400vac low voltage distribution cb supply 1 control unit 22 400vac low voltage distribution control unit 22 400vac low voltage distribution high pressure pump.bearing de control unit 22 400vac low voltage distribution stillstand heaters control control unit 22 400vac low voltage distribution oil mist suction device de control unit 22 400vac low voltage distribution h press pu.bearing de stand-by control unit 22 400vac low voltage distribution oil mist suction device nde control unit 22 400vac low voltage distribution indication unit 22 400vac low voltage distribution automatik input unit 22 400vac low voltage distribution circuit diageam unit…400vac low voltage distribution front view unit…400vac low voltage distribution inside view unit…400vac low voltage distribution cubicle view gauge board cover sheet gauge board front view gauge board inside layout gauge board fower supply gauge board cubicle lighting/heating gauge board 24vdc distributidn gauge board fault indication lamps gauge board fault messages to control gauge board temperatures thrust bearing gauge board temperatures de/nde guiide bearing gauge board temperatures de/nde bearing oil reservoir gauge board temperatures cold air from coolers gauge board display temperatures cooler 1-10 gauge board display temperatures cooler 11-20 gauge board temperatures hot air from coolers gauge board display tem.

单位 22 400 vac 低的电压分配钢笔 busbar单位 22 400 vac 低的电压分配小卧室 lighting.plug 和暖气单位 22 400 vac 低的电压分配测定和 ac-分配单位 22 400 vac 低的电压分配 220 vdc control2单位 22 400 vac 低的电压分配控制线路断路器单位 22 400 vac 低的电压分配自动机械 interconnection2单位 22本装置目录单位 22 400 vac 低的电压分配小卧室 lighting.plug anh 暖气单位 22 400 vac 低的电压分配 ac/直流补给控制单位 22 400 vac 低的电压分配 cb 补给 1 控制单位 22 400 vac 低的电压分配 cb 联结控制单位 22 400 vac 低的电压分配 cb 补给 2 控制单位 22 400 vac 低的电压分配 cb 补给 1 控制单位 22 400 vac 低的电压分配控制单位 22 400 vac 低的电压分配高度强迫 pump.bearing de 控制单位 22 400 vac 低的电压分配 stillstand 加热器控制控制单位 22 400 vac 低的电压分配油雾吸装置 de 控制单位 22 400 vac 低的电压分配 h 杂志报纸 pu.bearing de 台子-被控制单位 22 400 vac 低的电压分配油雾吸装置 nde 控制单位 22 400 vac 低的电压分配指示单位 22 400 vac 低的电压分配 automatik 输入单位 22 400 vac 低的电压分配线路 diageam单位…400休假低的电压分配前面视野单位…400休假低的电压分配内部看单位…400休假低的电压分配小卧室视野精确计量董事会掩护张精确计量董事会比较前面的视野标准度量在地面区划之内搭乘标准度量董事会 fower 补给标准度量董事会小卧室照明/暖气标准度量董事会 24 vdc distributidn标准度量董事会过失指示灯精确计量董事会过失信息控制标准度量董事会温度插入了举止标准度量董事会温度 de/ nde guiide 举止生的标准度量董事会温度 de/ nde 涂油水于水库标准度量董事会来自冷却器的温度冷空气精确计量董事会展览温度冷却器 1-10精确计量董事会展览温度冷却器 11-20标准度量董事会来自冷却器的温度热气精确计量董事会展览 tem。

When I started coding, I had problems with the ASF file writer.

当我开始编码，我曾与ASF文件作家的问题。

I know that in one of the schools the boys have risen up in the classroom and enlisted in a mass.

如果你们也和他们一样，是集体自愿的话，你们的老师将是多么地光荣啊。