查询词典 control assay

objective to establish immunological methods specific for detecting antigens in different groups of monoclonal antibodies.methods indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of aspergillus fumigatus,respectively.two different double monoclonal antibody sandwich elisa assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of aspergillus,penicillium marneffei,and 5 species of candidas.results the results of indirect immnofluorescence assay indicated that the monoclonal antibodies prepared with crude antigen of aspergillus fumigatus were specific for antigens in both clinical isolates and environmental isolates of aspergillus, whereas the other group of monoclonal antibodies was proved to be specific for aspergillus fumigatus of both clinical and environmental isolates.the elisa assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of aspergllius, while the other assay could only detect aspergillus fumigatus of both clinical and environmental isolates.and no cross reaction with the cell culture of penialllium marneffei and candidas was observed with the two methods.conclusion the elisa assays can detect both of the clinical and environmental isolates of aspergillus,and differentiate aspergillus fumigatus from other species of aspergillus.

目的 用2组曲霉单克隆抗体建立特异性识别不同种类曲霉抗原的检测方法。方法采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心elisa法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。结果间接免疫荧光显示，用天然烟曲霉抗原免疫获得的单克隆抗体(mabs-1)可广谱识别多种曲霉分离株，而重组烟曲霉抗原获得的单克降抗体(mabs-2)仅能特异性结合临床和环境分离的烟曲霉抗原。用mabs-1建立的双抗体夹心elisa法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mabs-2)建立的双抗体夹心elisa法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心elisa法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

Result The nested PCR assay was specific and there is no cross-reaction with other parasites, such as Eimeria spp., Cryptosporidium spp., Giardia sp., Toxoplasma sp., Trichuris sp. and cattle ciliate. The assay was able to detect as low as 2.85 × 10^(-2) fg of control positive DNA. The results of detection of clinical samples revealed that the assay was coinciding with microscopic examination.

该套式PCR能特异性地扩增出牛源环孢子虫基因组DNA中相应片段，与艾美耳球虫、隐孢子虫、贾第虫等10种相关原虫基因组DNA无交叉反应；最低能检测到2.85×10^(-2)fg的阳性参照DNA；对临床样品检测结果表明PCR技术检查阳性者显微镜检查都为阳性。

The book is divided into 10 chapters, including discrete and continuous system of PID control system, commonly used digital PID control, Expert PID and fuzzy PID control, neural PID control, genetic algorithm PID control, Multivariable decoupling PID control, several advanced PID control, gray PID control, PID control servo system, PID control in real time, ea

全书共分十章，包括连续系统和离散系统的 PID 控制，常用数字 PID 控制，专家 PID和模糊 PID 控制，神经 PID 控制，遗传算法 PID 控制，多变量解耦 PID 控制，几种先进的PID 控制，灰色 PID 控制，伺服系统 PID 控制，PID 实时控制，每种方法都通过 MATLAB 仿真程序进行了说明。

The results showed: no significant difference was found between the results of single tube quantitative assay method and multiple tube fermentation technique;single tube quantitative assay method showed better accurateness,precision,and reproducibility than multiple tube fermentation technique;single tube quantitative assay method was quicker and more economical.

结果表明：MUG单管定量检测法与大肠埃希氏菌多管发酵法对菌悬液、天然海水、水产品的检测结果没有统计学差异；MUG单管定量检测法检测数据的精确性高于大肠埃希氏菌多管发酵法，而且更为快速、经济。

Significant Spearman rank correlations were found between the results obtained by Luminex assay and by chemiluminescence immunoassay or time-resolved fluorescence immunoassay. Only 1 microliter of serum and 3 hours were needed for Luminex assay. With the advantages of unique capability of multiplexing and high-throughput, good reproducibility, increased detection range and sensitivity, Luminex assay would have a great potential in clinical application.

本研究应用液相芯片技术利用双抗体夹心免疫分析原理同时检测了人血清中的癌胚抗原、甲胎蛋白和乙肝表面抗原，评价了该方法的线性范围、最低检测限、精密度、灵敏度、特异度、准确度等指标，并对检测结果与化学发光免疫分析法（chemiluminescence immunoassay，CLIA）和时间分辨荧光免疫分析法（time-resolved fluorescence immunoassay，TRFIA）进行了相关性分析。

The calibration curve of the SM2-Kit with standard SM2 inhibitor was typical sigmoid curve fitted to the four parameter logistic equation with the linear detection of 0.625 μg/L to 80.0 μg/L (R2=0.9911), the sensitivity of 0.9 μg/L, the IC50 of 5.82 μg/L and the detection limit of 1 μg/L. The recoveries of SM2 spiked in pig feed were 83.4 %, in pig urine were 89.5 %. The precision and accuracy of the assay as determined by inter-assay and intra-assay coefficient variation was both below 15 %. The SM2-Kit generally had 2.08 % cross-reactivity towards sulfamerazine and little or no cross-reactivity towards other sulfonamides. The dilution solution of SM2 had no effect on results of SM2-Kit. The validity of SM2-Kit in 4 ℃ was above six months.

研究结果表明，SM2-Kit的标准曲线呈典型的S型，相关系数R2=0.9911，符合4参数logit曲线拟合，线性检测范围为0.625 μg/L～80 μg/L，灵敏度为0.9 μg/L，半数抑制浓度(IC50)为5.82 μg/L，检测限为1 μg/L；饲料样、猪尿样的平均添加回收率分别为83.4 %、89.5 %，平均批内和批间变异系数均低于15 %；SM2-Kit与磺胺甲基嘧啶的交叉反应率为2.08 %，与其它磺胺类药几乎没有反应性；基质对SM2-Kit的检测结果影响不大；试剂盒在4 ℃可保存6个月。

Proliferation assay, cellular cycle and apoptosis analysis, invasion and migration assay and adherency assay showed that GEBP11 could inhibit the proliferation of Co-HUVECs and HUVECs, induce the apoptosis of ECs, but not alter the cell cycle of ECs.

MTT细胞增殖实验、细胞周期及凋亡分析、细胞侵袭迁移实验、细胞粘附实验显示，GEBP11短肽对Co-HUVECs及HUVECs的增殖表现出不同程度的抑制作用，对Co-HUVECs抑制作用更强，GEBP11能诱导内皮细胞的凋亡，而对细胞周期无明显影响。

Methods: Human T cell leukemia cell line Jurkat was chosen as a model. The effect of deguelin on the growth of Jurkat cells was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium assay. Apoptosis was detected through DNA fragmentation assay, Hoechst 33258 staining assay and Annexin V/PI double-labeled cytometry. The expression levels of nucleophosmin and nucleoporins, including Nup88 and Nup214, were studied by flow cytometry, Western Blot and reverse transcription-polymerase chain reaction.

以人类T淋巴细胞白血病细胞系Jurkat作为研究对象，采用MTT法检测细胞增殖活性；DNA ladder法、Hoechst33258染色法和Annexin V-FITC/PI双标法检测细胞凋亡；流式细胞术、Western Blot、RT-PCR检测鱼藤素作用前后，Jurkat细胞内核孔蛋白Nup88、Nup214与核磷蛋白(nucleophosmin， NPM)表达水平的变化；激光共聚焦显微技术观察上述核孔蛋白与核磷蛋白的亚细胞定位情况。

The immune function was evaluated with T lymphocyte proliferation,NK cell activity assay,IgMplaque forming cell assay,blood serum erythrocytolysin assay and delayedtypehypersensitivity to SRBC.

采用T淋巴细胞增殖和迟发型变态反应来评价细胞免疫功能，采用血清溶血素测定和抗体生成细胞检测来评价体液免疫功能，采用乳酸脱氢酶法测定自然杀伤细胞活性来评价非特异性免疫功能。

Methods The mouse models bearing S180 sarcoma were treated with cadmium chloride with dosages of 0.25,1.00 and 4.00 mg.kg-1,the anti-tumor effect was evaluated by calculating of tumor weight;spleen and thymus indexes,carbon phage assay,lymphocyte transformed assay and hematolysis assay were used to measure the effect of cadmium chloride on immune function.

应用0.25、1.00及4.00 mg.kg-1的氯化镉处理S180肉瘤小鼠模型，检测瘤重确定氯化镉对S180肉瘤的抑瘤作用，采用胸腺指数、脾脏指数、碳粒廓清试验、淋巴细胞转化实验、半数溶血素实验检测小鼠免疫功能的变化。

Continuously improve the production mechanism, to provide customers with better quality products and services.

不断的完善生产机制，给客户提供更优质的产品及服务。

B:Yes, he was the very prize-winner in the court tennis ten years ago?

是的，他是十年前的世界网球冠军。