查询词典 control assay

Objective: To investigate the effects of sulindac on the growth and motility of ovarian cancer cell SKOV3 and its involved mechanism. Methods: In order to detect the effects of sulindac on cultured SKOV3, sulindac with different concentration and untreated control were set up. The changes in morphology of SKOV3 were observed by phase contrast microscopy. MTT colorimetric assay was used to study the effects of sulindac on SKOV3 cell growth. Motility of SKOV3 was determined by scarification assay.

目的：研究舒林酸对卵巢癌SKOV3细胞的生长和细胞运动的影响以及对基质金属蛋白酶2（MMP2）、环氧合酶-2(COX-2)表达的影响：方法：将不同浓度的舒林酸加入培养液中观察其对SKOV3细胞形态的影响，采用MTT法观察舒林酸对SKOV3细胞生长的影响，通过划痕试验观察舒林酸对SKOV3细胞运动的影响，利用免疫细胞化学方法研究舒林酸对SKOV3细胞MMP2、COX-2表达的影响。

Methods: in order to detect the effects of sulindac on cultured skov3, sulindac with different concentration and untreated control were set up. the changes in morphology of skov3 were observed by phase contrast microscopy. mtt colorimetric assay was used to study the effects of sulindac on skov3 cell growth. motility of skov3 was determined by scarification assay.

目的：研究舒林酸对卵巢癌skov3细胞的生长和细胞运动的影响以及对基质金属蛋白酶2（mmp2）、环氧合酶-2(cox-2)表达的影响：方法：将不同浓度的舒林酸加入培养液中观察其对skov3细胞形态的影响，采用mtt法观察舒林酸对skov3细胞生长的影响，通过划痕试验观察舒林酸对skov3细胞运动的影响，利用免疫细胞化学方法研究舒林酸对skov3细胞mmp2、cox-2表达的影响。

Impact of PCL capsulation on the mesenchymal stem cell activity studied in vivo, one plate culture with complete culture medium for control group with and two encapsulated transplantation for experimental groups(complete culture medium or basic culture medium for cell suspended) installed,after package and transplantation,retrieving capsulated cells for cell count,MTT assay,CCK-8 assay Daily for 8days to determine cell activity,and analysis growth curve.

以完全培养液平皿法细胞培养为对照组，设2个实验组进行聚己内酯囊管化骨髓间充质干细胞移植细胞活性研究。两个实验组分别以完全培养液和基础培养液悬浮细胞后封装移植，囊管内细胞回收后以细胞计数、MTT法、CCK-8法测定细胞的活性，共8天，进行生长曲线分析。

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

Plan control, information control and rules control are considered in this assay for FAWs strategy control.

本论文主要是从计划控制、信息控制和规章制度控制三个方面，为一汽提出实施控制的一些建议。

In the last chapter,we study the control problems of the distributedparameter system governed by a class of higher order pseudohyperbolicequation related to the symmetric regularized long wave equation.we havediscussed the controllability and optimal control problems.we have obtainedcompatibility condition of the constraint when the state is controllable,therepresentation of the optimal control for both the time optimal control problemand the optimal control problem of minimum energy type,and the equationsatisfied by the minimum time.we have shown that the optimal control belongsto the boundary of the admissible control set.

在第六章中，我们研究了与对称正则长波方程相关的一类高阶拟双曲型方程支配的分布参数系统的控制问题。讨论了系统的状态能控性问题和最优控制问题，给出了状态能控时的约束相容性条件，给出了最速控制问题、最小能量型最优控制问题的最优控制表达式和最速时间所满足的方程式，证明了最优控制属于控制集合的边界。

The paper, first of all, particularly expounded the new characteristics of the control within the accountants of the enterprises on the accounting computerization basis-control object dualization, control key points singularity, control form procedure, control content data, control range magnifying, and control technique modernization; then put forward the new methods of increasing the enterprises.

具体地阐述了在会计电算化条件下，企业会计内部控制的新特点———控制对象二元化、控制重点单一化、控制形式程序化、控制内容数据化、控制范围扩大化、控制技术现代化；其次提出了企业会计内部控制应增加的新方法，并作了相关的建议。

Results: For CRF rats which made by fed with feedingstuff contains 0.75% of adenine for 4 weeks, the weight of model control group rats were lighter than that of Yi Shen Granule groups rats. The water wastage of Yi Shen Granule groups rats were less than that of model control group rats. Compared urine volume, the ejectable quantity of Na, K, Cl and proteinuria in 12 hours , the data of Yi Shen Granule groups rats were less than that of model control rats. The specific gravity of Yi Shen Granule groups rats was higher than that of model control rats. The numerical value of RBC, HGB, HCT, MCV, MCH in model control group was lower than that in Yi Shen Granule groups. The content of BUN and Cre in serum of model control groups rats were higher than that of Yi Shen Granule groups. The results of excretion test of phenolsulfonphthalein showed that the RPF of Yi Shen Granule groups rats was larger than that of model control group rats.

结果： 益肾颗粒可以使0.75%腺嘌呤饲喂法致大鼠慢性肾衰模型动物体重增长加快；给药后观察，给药组动物饮水量较少，12小时尿量、12小时钠、氯、尿蛋白排泄总量均显著低于模型组动物，尿液比重值较大；模型组动物的RBC、HGB、HCT、MCV、MCH等指标的数值较给药组动物的数值小；给药组动物血清中BUN和Cre的含量较低；给药组动物酚红排泄率均高于模型组动物；给药30天，处死各组动物，尸解观察，模型对照组动物肾脏呈灰白色，各给药组动物肾脏表面均出现不同程度的红、白相兼的颗粒状纹理，各组动物肾脏均肿大，切面可见不同程度的腺嘌呤结晶沉积。

The aerodynamics characteristic of the innovative control surfaces which are implemented on the flying wing aircraft with large aspect ratio were introduced, and elevon and split rudder were involved. And the control effects on three axis were analyzed basing on computetional fluid dynamics method. The concept of control volume was imported to introduce parameters-based method of control surfaces design. The control effect requirements of the control surfaces were also evaluated. The original design of certain aircraft's control surfaces was modified.

介绍了在该新布局飞机上采用的升降副翼和开裂式方向舵等新型操纵面的气动特点，并在计算空气动力学的基础上分析了其三轴控制效率，引进舵容量的概念提出了操纵面的参数化设计方法，基于飞机的可控性对各新型操纵面的操纵效率需求进行了评估，最终对某型飞机的操纵面初步设计进行了修正。

In the design of automatic control system for isomerization unit from heptane tower of PX devices, single-loop control is used in the control programme. There is a total of 11 circuit , of which is 8 single-loop control system and 3 cascade control system. It has done the choice and relevant calculation on the controller, control valves and measurement devices used in the control system. There is a total of eight chapters in this design.

在对PX装置异构化单元脱庚烷塔自动控制系统进行设计时，控制方案基本采用单回路控制，总共有11个设计回路，其中单回路控制系统8个，串级控制系统3个，并对控制系统所要用到的控制器，控制阀和测量元件等进行了选择且进行了相关的计算。

A historical region of southwest France between the Pyrenees and the Garonne River. The duchy of Aquitaine was joined with France after the marriage of Eleanor of Aquitaine to King Louis VII in1137, but its possession was disputed after her subsequent marriage to Henry II of England.

阿基坦法国西南部一个历史地区，位于比利牛斯山和加隆河之间，在1137年阿基坦的埃莉诺与国王路易斯七世结婚之后阿基坦公国加入法国，但当她再嫁英国亨利二世后，其归属权便受到争议

There are moments when I look at the smiling , happy faces of my expectant staff and wonder how they will cope when confronted by an outraged housemaster along with an unrepentant and recalcitrant bleached blond teenager in 18 years ' time .

有时候，我看着那些满怀期待的员工微笑，幸福的脸，想着当他们遇到一位愤怒的宿舍管理员和一位不知悔改，叛逆，而且将头发染成白色的18岁少年时，他们会如何处理。