control assay
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The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.
应用杂交瘤技术制备ZNRD1的首个单克隆抗体;2)利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达;3)构建ZNRD1的小干扰RNA载体,并测序鉴定;4)利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞(AGS、SGC7901、MKN28)和小鼠成纤维细胞(NIH3T3),G418筛选后进行鉴定;5)利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞(SGC7901)、正常胃组织上皮细胞(GES-1)、对长春新碱耐药的胃癌细胞(SGC7901/VCR)、药敏白血病细胞(HL-60)、对长春新碱耐药的白血病细胞(HL-60/VCR),G418筛选后进行鉴定;6)利用MTT实验检测ZNRD1高/低表达对细胞(AGS、SGC7901、MKN28、NIH3T3、GES-1)生长的影响;7)通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响;8)通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响;9)通过流式细胞仪分析ZNRD1高/低表达对细胞(AGS、MKN28、NIH3T3、GES-1)的细胞周期的影响;10)通过流式细胞仪分析上调ZNRD1对细胞(AGS、MKN28、NIH3T3)的凋亡的影响;11)通过MTT实验检测ZNRD1高/低表达对细胞(SGC7901、SGC7901/VCR、HL-60、HL-60/VCR)体外药物敏感性的影响;12)通过肾包膜下移植法检测ZNRD1高/低表达对细胞(SGC7901、SGC7901/VCR)体内药物敏感性的影响;13)通过流式细胞仪分析ZNRD1高/低表达对细胞(SGC7901、SGC7901/VCR、HL-60、HL-60/VCR)内阿霉素蓄积和泵出的影响;14)通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响;15)通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞(SGC7901、SGC7901/VCR、HL-60)凋亡敏感性的影响;16)通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响;17)利用RT-PCR、Western blot对基因芯片的结果进行鉴定;18)利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用;19)利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用;20)利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响;21)利用反义核酸技术抑制p21的表达;通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响;22)利用反义核酸技术抑制p27的表达;通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响;23)利用脂质体转染法上调Skp2的表达;通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响;24)利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响;25)利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响;26)利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响;27)利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞(SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR)多药耐药表型的影响;利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。
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PART 1 UNIT 1 B Electrical and Electronic Engineering Basics A Electrical Networks ———————————— 3 Three-phase Circuits A The Operational Amplifier ——————————— 5 UNIT 2 B Transistors A Logical Variables and Flip-flop —————————— 8 UNIT 3 B Binary Number System A Power Semiconductor Devices —————————— 11 UNIT 4 B Power Electronic Converters A Types of DC Motors —————————————15 UNIT 5 B Closed-loop Control of DC Drivers A AC Machines ———————————————19 UNIT 6 B Induction Motor Drive A Electric Power System ————————————22 UNIT 7 B PART 2 UNIT 1 B Power System Automation Control Theory A The World of Control ————————————27 —————29 The Transfer Function and the Laplace Transformation UNIT 2 B A Stability and the Time Response ————————— 30 Steady State————————————————— 31 A The Root Locus ————————————— 32 ————— 33 UNIT 3 B The Frequency Response Methods: Nyquist Diagrams UNIT 4 A The Frequency Response Methods: Bode Piots ————— 34 B Nonlinear Control System 37 UNIT 5 A Introduction to Modern Control Theory B State Equations 40 38 UNIT 6 A Controllability, Observability, and Stability B Optimum Control Systems UNIT 7 A Conventional and Intelligent Control B Artificial Neural Network Computer Control Technology A Computer Structure and Function 42 B Fundamentals of Computer and Networks 43 44 PART 3 UNIT 1 UNIT 2 A Interfaces to External Signals and Devices B The Applications of Computers 46 UNIT 3 A PLC Overview B PACs for Industrial Control, the Future of Control UNIT 4 A Fundamentals of Single-chip Microcomputer 49 B Understanding DSP and Its Uses 1 UNIT 5 A A First Look at Embedded Systems B Embedded Systems Design Process Control A A Process Control System B 50 PART 4 UNIT 1 Fundamentals of Process Control 52 53 UNIT 2 A Sensors and Transmitters B Final Control Elements and Controllers UNIT 3 A P Controllers and PI Controllers B PID Controllers and Other Controllers UNIT 4 A Indicating Instruments B Control Panels Control Based on Network and Information A Automation Networking Application Areas B Evolution of Control System Architecture PART 5 UNIT 1 UNIT 2 A Fundamental Issues in Networked Control Systems B Stability of NCSs with Network-induced Delay UNIT 3 A Fundamentals of the Database System B Virtual Manufacturing—A Growing Trend in Automation UNIT 4 A Concepts of Computer Integrated Manufacturing B Enterprise Resources Planning and Beyond Synthetic Applications of Automatic Technology A Recent Advances and Future Trends in Electrical Machine Drivers B System Evolution in Intelligent Buildings PART 6 UNIT 1 UNIT 2 A Industrial Robot B A General Introduction to Pattern Recognition UNIT 3 A Renewable Energy B Electric Vehicles UNIT 1 A
电路 2 电路或电网络由以某种方式连接的电阻器,电感器和电容器等元件组成。如果网络不包含能源,如电池或发电机,那么就被称作无源网络。换句话说,如果存在一个或多个能源,那么组合的结果为有源网络。在研究电网络的特性时,我们感兴趣的是确定电路中的电压和电流。因为网络由无源电路元件组成,所以必须首先定义这些元件的电特性。就电阻来说,电压-电流的关系由欧姆定律给出,欧姆定律指出:电阻两端的电压等于电阻上流过的电流乘以电阻值。在数学上表达为: u=iR (1-1A-1)式中 u=电压,伏特;i =电流,安培;R =电阻,欧姆。纯电感电压由法拉第定律定义,法拉第定律指出:电感两端的电压正比于流过电感的电流随时间的变化率。因此可得到:U=Ldi/dt 式中 di/dt =电流变化率,安培/秒; L =感应系数,享利。电容两端建立的电压正比于电容两极板上积累的电荷 q 。因为电荷的积累可表示为电荷增量 dq 的和或积分,因此得到的等式为 u=,式中电容量 C 是与电压和电荷相关的比例常数。由定义可知,电流等于电荷随时间的变化率,可表示为 i = dq/dt。因此电荷增量 dq 等于电流乘以相应的时间增量,或 dq = i dt,那么等式(1-1A-3)可写为式中 C =电容量,法拉。
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PART 1 Electrical and Electronic Engineering Basics UNIT 1 A Electrical Networks B Three-phase Circuits UNIT 2 A The Operational Amplifier ——————————— 5 B Transistors UNIT 3 A Logical Variables and Flip-flop —————————— 8 ———————————— 3 B Binary Number System UNIT 4 A Power Semiconductor Devices —————————— 11 B Power Electronic Converters UNIT 5 A Types of DC Motors —————————————15 B Closed-loop Control of DC Drivers UNIT 6 A AC Machines ———————————————19 B Induction Motor Drive UNIT 7 A Electric Power System ————————————22 B Power System Automation PART 2 Control Theory UNIT 1 A The World of Control ————————————27 B The Transfer Function and the Laplace Transformation UNIT 2 A B —————29 Stability and the Time Response ————————— 30 ————————————— 32 Steady State————————————————— 31 UNIT 3 A The Root Locus B The Frequency Response Methods: Nyquist Diagrams ————— 33 UNIT 4 A The Frequency Response Methods: Bode Piots ————— 34 B Nonlinear Control System 37 UNIT 5 A Introduction to Modern Control Theory B UNIT 6 State Equations 40 38 A Controllability, Observability, and Stability B Optimum Control Systems UNIT 7 A Conventional and Intelligent Control B Artificial Neural Network PART 3 UNIT 1 Computer Control Technology A Computer Structure and Function B 42 43 44 Fundamentals of Computer and Networks UNIT 2 A Interfaces to External Signals and Devices B The Applications of Computers 46 UNIT 3 A PLC Overview B PACs for Industrial Control, the Future of Control 1 UNIT 4 A Fundamentals of Single-chip Microcomputer B Understanding DSP and Its Uses 49 UNIT 5 A A First Look at Embedded Systems B Embedded Systems Design PART 4 UNIT 1 Process Control A A Process Control System 50 B Fundamentals of Process Control 53 52 UNIT 2 A Sensors and Transmitters B Final Control Elements and Controllers UNIT 3 A P Controllers and PI Controllers B PID Controllers and Other Controllers UNIT 4 A Indicating Instruments B Control Panels PART 5 UNIT 1 Control Based on Network and Information A Automation Networking Application Areas B Evolution of Control System Architecture UNIT 2 A Fundamental Issues in Networked Control Systems B Stability of NCSs with Network-induced Delay UNIT 3 A Fundamentals of the Database System B Virtual Manufacturing—A Growing Trend in Automation UNIT 4 A Concepts of Computer Integrated Manufacturing B Enterprise Resources Planning and Beyond PART 6 UNIT 1 Synthetic Applications of Automatic Technology A Recent Advances and Future Trends in Electrical Machine Drivers B System Evolution in Intelligent Buildings UNIT 2 A Industrial Robot B A General Introduction to Pattern Recognition UNIT 3 A Renewable Energy B Electric Vehicles 2 UNIT 1 A
电路 电路或电网络由以某种方式连接的电阻器,电感器和电容器等元件组成。如果网络不包含能源,如电池或发电机,那么就被称作无源网络。换句话说,如果存在一个或多个能源,那么组合的结果为有源网络。在研究电网络的特性时,我们感兴趣的是确定电路中的电压和电流。因为网络由无源电路元件组成,所以必须首先定义这些元件的电特性。就电阻来说,电压-电流的关系由欧姆定律给出,欧姆定律指出:电阻两端的电压等于电阻上流过的电流乘以电阻值。在数学上表达为: u=iR (1-1A-1)式中 u=电压,伏特;i =电流,安培;R =电阻,欧姆。纯电感电压由法拉第定律定义,法拉第定律指出:电感两端的电压正比于流过电感的电流随时间的变化率。因此可得到:U=Ldi/dt 式中 di/dt =电流变化率,安培/秒; L =感应系数,享利。电容两端建立的电压正比于电容两极板上积累的电荷 q 。因为电荷的积累可表示为电荷增量 dq 的和或积分,因此得到的等式为 u=,式中电容量 C 是与电压和电荷相关的比例常数。由定义可知,电流等于电荷随时间的变化率,可表示为 i = dq/dt。因此电荷增量 dq 等于电流乘以相应的时间增量,或 dq = i dt,那么等式(1-1A-3)可写为式中 C =电容量,法拉。
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aldosterone assay:[醛固酮测定]
?plasma cortisol assay[血浆皮质醇测定] | ?aldosterone assay[醛固酮测定] | ?corticosterone assay[皮质酮测定]
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Antithrombin III assay:抗血栓III化验
864.7040 三磷酸腺苷释放化验 Adenosine triphosphate release assay | 864.7060 抗血栓III化验 Antithrombin III assay | 864.7100 红血球酶化验 Red blood cell enzyme assay
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assay bar:检定金,鉴定棒
assay balance 试金天平 | assay bar 检定金,鉴定棒 | assay foot 分析英尺