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Methods BMSCs isolated from rabbit iliac bone marrow were culture-expanded in vitro. The injectable tissue-engineered bone constructed from autologous PRP, FG, and BMSCs was cultured in vitro, and its biological characteristics were observed including the time of gel formation, histological features, seed cell survival and microscopic structures. Results The constructed injectable tissue-engineered bone began gel formation within 20 to 30 s, and after a week-long culture, the gelatine began to degrade, and numerous well viable fusiform cells could be seen to adhere to the bottom of the Petil dish, Scanning electron microscopy identified globular and olivary cells embedded in the fibrin glue, and numerous small particles could be seen around of the cells.

从兔髂骨处抽取骨髓体外培养BMSCs并诱导向成骨细胞分化，抽取兔自体动脉血提取PRP，以FG、BMSCs、PRP共同构建可注射型组织下程骨并体外培养观察其生物学特性如凝胶形成时间，组织学特点、细胞存活情况及其超微结构特征等结果构建的可注射型组织工程骨可在短时间内形成凝胶，体外培养1周时其中细胞生长良好，电镜下见纤维蛋白网格结构致密，种子细胞及血小板颗粒分布广泛。

Compound 4 exhibits an unusual 2-D (4,6)-lattice-type network constructed by the [Co222]2- dimers and [Co(H2O)2]2+ units. As for Compound 5, four basic units link to each other form an approximately parallelogrammic pore, which constructed a 2-D covalent bond double-layers network.

化合物4展现出由[Co_2_2_2]~(2-)二聚体和[Co(H_2O)_2]~(2+)组成的具有4、6两种格子的不同寻常的二维网状结构，化合物5以类平行四边形孔道为基本单元构筑了二维共价键网络结构。

Methods: We constructed a recomb ined adenoviral vector carrying CD or LacZ. 293 packaging cell was trans fected by the newly-constructed plasmid and generated the infectious virus.

构建含CD或LacZ基因的重组腺病毒载体，在293细胞内重组包装后，体外感染小鼠结肠癌细胞株C26，并给予不同浓度的前药氟胞嘧啶(5-FC)进行肿瘤杀伤效果观察。

The late phase expression cassette suited for adenovirus vectors was also constructed. The HBV S gene, HBV preS2+S gene and the late phase expression cassette +HBV S genes were respectively cloned into the Ad5 vector downstream of the E3 promoter. Semiconfluent monolayers of 293 cells were cotransfected with the above constructed plasmids and Ad5 DNA EcoRI A fragment. The progeny adenoviruses named rAd5S, rAd5MS, rAd5S2S were harvested.

通过共转染优化实验，建立了脂质体转染腺病毒的有效方法，并构建了适合腺病毒载体的晚期表达盒，进一步将乙型肝炎表面抗原基因、HBV preS2+S、腺病毒晚期表达盒+HBV S基因插入E3区，与EcoRI消化的Ad5 DNA A片段共转染细胞，并获得重组腺病毒，相应命名为：rAd5S、rAd5MS、rAd5S2S。

Methods Amplify IL-6 (23)-PE40 gene from the previously constructed recombinant plasmid pKK-IL-6(23)-PE40 by PCR, and insert to the SnaB Ⅰand NotⅠ sites of vector pPIC9. Transform the constructed recombinant plasmid pPIC0-SN to P. pastoris GS115 by electroporation, screen Mut-recombinants for expression under induction of methanol.

采用PCR技术，将目的基因IL-6(23)-PE40插入到pPIC9载体中SnaBⅠ与NotⅠ位点，电转化至巴斯德毕赤酵母GS115中，筛选Mut-型重组酵母；甲醇诱导表达，体外细胞试验检测其细胞毒性。

To assess the invasion risk of alien marine species, an invasive risk assessment system was constructed based on the characters of introduced marine species and on the analysis of literatures and expert consultancy. The system has 5 primary and 20 secondary indexes, and was constructed using analytic hierarchy process method and trigonometrical fuzzy numbers for determining the weight of each index of the system.

中文摘要：为了评估外来海洋物种入侵的风险，根据外来海洋物种入侵特点，在文献分析和专家咨询的基础上，采用层次分析和三角模糊数数值标度计算权重的方法，设计5个一级指标和20个二级指标，构建了外来海洋物种入侵风险评估体系。

A kind of M-TNF recombin ant with a stable,uncleavable mutant typewas constructed for its gene therap y. Methods: On the basis of cloning the M-TNF c D NA encoding region,the authors constructed mTNFm,which could potentially expre ss on the c ell surface with a uncleavable type of TNF resulting in deletion of the sequence encoding enzymatic cleavage site of TNF-α-converting enzyme.

近年来研究表明，T NF的两种形式在受体结合、生物学效应等方面各具特征［3，4］。M-TNF通过直接接触在组织局部中发挥效应的特性，有望克服S-TNF在生物治疗应用中引起的远端及全身性毒副作用。

A dendrogram constructed by an unweighted pair group method of cluster analysis showed that populations from Panan constructed one branch and separated from other populations.

根据POPGEN软件计算得到的群体之间的遗传距离进行聚类分析，结果显示浙江的各栽培群体有较近的遗传关系，与其他群体已存在遗传分化。

At last,a convergent and general protocol to marine natural product,as demonstrated by the enantioselective total synthesis of malyngamides O,P,Q,R, 5"-epi-Q and 5"-epi-R bearing terminal vinylic chloride structure,was developed in detail.E,(7S)-7-methoxytetradec-4-enoic acid was prepared using the above described Johnson-Clainsen rearrangement,the terminal vinyl chloride was constructed using Wittig reaction as key step,the pyrrolidone derivative was constructed using L-serine as natural chiral pool.Starting from the synthesis of malyngamides O and P to explore the general route,an aldol reaction of methyl acetate with the vinyl chloride part in the presence of LDA gave the skeleton of amine portion,followed by amidation with the fatty acid finished the synthesis of malyngamide P.

先以malyngamides O和P为试点探索通用合成路线，将乙酸甲酯和烯氯部分在LDA存在下进行aldol反应得到氨部分的骨架，再将手性不饱和脂肪酸和氨部分进行酰胺化反应完成了malyngamide P的合成，将P在碱性条件下进行烯醇甲基化反应完成了malyngamide O的全合成。

That is, when a web site is constructed, the corresponding administration and maintenance system must be constructed to sustain the operation of the whole web site. Thus every man can administrates the web site and releases information everyday.

在建立前台信息发布网站的同时，还要建立一套具有友好用户界面的后台管理维护系统，优化整个网站的运行，帮助普通用户对前台的网站进行日常管理和信息发布。

There had been a moment during the breakfast meeting, though, after the backslapping and the small talk and when all of us were seated, with Vice President Cheney eating his eggs Benedict impassively and Karl Rove at the far end of the table discreetly checking his BlackBerry, that I witnessed a different side of the man.

那次早餐会期间，在表示过关心和寒暄以后，我们所有人坐了下来，我注意观察了一下，副总统切尼面无表情地吃着班尼迪克蛋（Eggs benedict，源自美国，以英式松饼、火腿、水煮蛋以及荷兰酱组合而成），卡尔。罗夫在餐桌的远端谨慎地经常查看他的黑莓手机，我目睹了他所不为人知的一面。

Talk Undelete failed; someone else may have undeleted the page first.

Talk 无法删除选定的页面或图像（它可能已经被其他人删除了）。