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And then,the article briefly analyzed the advantages and disadvantages of the subjectiveevaluation method. According to the fact of the research, it confirmed to simulate darkvision environment of landscape illumination in the lab by the way of the subjectiveevaluation investigation. I classified he confirmed homochromy chromatic lightsaccording their brightness, and used the computer projection system which had beenmeasured to project and reappearance, then carry on the subjective evaluationexperiment of chromatic light feeling preference faced to the people of big swatch.

而后简要分析了主观评价方法的优缺点，结合该研究的实际情况确定利用主观评价调查的方法在实验室模拟景观照明的暗视觉环境，将已确定的单色色光按明度分级，通过已完成标定的计算机投影系统投射再现，对大样本的受试人群进行色光心理感受倾向性的主观评价测试实验。

Among them strain MZ1 had been confirmed a lysogenic strain by indicator strain ZK1 after producing phage plaques induced by Mitomycin C.Two templates of overnight cultivation and its DNA from strain MZ1 were then amplified in the same time with five pairs of primers designed from the same phage,and the same products were obtained,suggesting that strain MZ1 was also confirmed a lysogenic strain by PCR method.

MZ1菌株的过夜培养液与指示菌ZK1(血清型H25)混匀并在双层琼脂上培养12 h后出现了滴度为2×107 pfu的噬菌斑，从而证明生产菌株MZ1确为溶原菌；又以GIL01同源性较高的其它四个基因设计引物，分别以MZ1的培养物及其溶原性噬菌体基因组为模板进行PCR扩增，结果两者得到几乎一致的带型，进一步证明了生产菌株MZ1为溶原菌，说明利用PCR法检测鉴定苏云金杆菌的溶原性是可行的。

Of the 19 cases,19 fetus were found,MRI confirmed 14 cases considered or suspected as fetal corpus callosum agenesis by US(8 cases are completed agenesis of corpus collosom-CACC,6 cases are partial agenesis of corpus collosom-PACC, and 5 cases had took on autopsy),however 3 cases are mild enlargement of lateral cerebral ventricle and 2 cases are leukodystrophy.In these cases,MRI confirmed a Dandy-Walker syndrome and a lipoma of corpus callosum suspected by US,detect a Dandy-Walker syndrome and a microcephalus made missed diagnoses by US.The fetal corpus callosum and additional cerebral anomalies were shown more clearly on MR T_2-weighted images.

胎儿胼胝体长度与额枕径的比值在24～36孕龄阶段相对较恒定。2、19例孕妇共检出胎儿19个，MRI证实超声疑诊的胎儿胼胝体发育不全14例(完全型胼胝体发育不全8例，部分型胼胝体发育不全6例，有胎儿尸检结果对照者5例)，3例为单纯侧脑室轻度扩张，2例考虑脑白质发育不良，合并Dandy—Walker综合征1例，胼胝体脂肪瘤1例，检出超声漏诊合并Dandy-Walker综合征1例及脑小畸形1例。

Results 32 cases of acoustic neurinoma,24 cases were large neurinoma,all confirmed by surgery,8 cases were small neurinoma,of which two cases were confirmed by surgery.

结果 32例听神经瘤，大瘤24例，均为手术证实，小瘤8例，其中2例为手术证实。

Results:92 patients (113 ears) were examined by PTHT, AI , 40 Hz AERP,ABR and DPOAE. It was found that organic deafness was confirmed on 98 ears (86.73%), of which exaggerated hearing loss was 38 ears; nonorganic deafness was confirmed on 13 ears (11.50%); stimulated deafness was 2 ears(1.77%). 98 organic deafness ears were diagnosed, of which conductive hearing loss was 25ears; sensorineural hearing loss was 29 ears, and mixed hearing loss was 44 ears.

结果： 92例患者113耳行纯音听阈测试、声导抗、40 Hz AERP、ABR与DPOAE检测，发现器质性聋98耳，占86.73%，其中夸大性聋者38耳，夸大程度＜20dBnHL6耳，20～40dBnHL27耳，＞40dBnHL5耳；非器质性聋13耳，占11.50%，诈聋2耳，占1.77%；98耳器质性听力损失者中，25耳为传导性耳聋，29耳为感音神经性耳聋，44耳为混合性耳聋。

French Minister of Health, homes on the 1st olona Torkan said that France has confirmed two cases of influenza A H1N1 influenza cases, this is the first time in France confirmed cases of this virus.

法国卫生部长罗斯利娜。巴舍洛。纳尔坎1日说，法国已经确诊2例甲型H1N1流感病例，这是法国首次确诊这种流感病例。

The new opinion and mechanism that the simultaneously adsorption of complex pollution of both organic and heavy metals will be achieved on soil modified with amphoteric surface modifying agent, which have both charged group and organophilic group on one ASMA molecule, were proposed at first time, and its rationality was testified at the same time; The 50% was the critical CTMAB modifying ratio of soil CEC at which the organophilic bond modifying mechanism was emerged; The thermodynamic characteristics of adsorption of OPT and HMP on OMS were discovered ; The study revealed the surface peculiarity of OMS that the modifying agent were distributed at uneven scatter mode and multi-characters surface were coexisted on surface of OMS, revealed the adsorption peculiarity of OMS that multi-mechanisms were coexisted, confirmed the unequal charge amount modifying mode of OMS in which dual-modifying mechanism of both ion exchange and OPB were processed at the same time, and confirmed that the adsorption hobby to CTMAB was higher than that to Ca2+ on Lou soil.

首次提出了用两性表面修饰剂对土壤进行修饰改性，利用两性表面修饰剂所具有的荷电基团和疏水基团达到对土壤中有机、重金属污染物同时吸附的思路和机理，并通过实验证实了所提设想的合理性；发现了土壤CEC值的50%修饰比例是CTMAB开始显现以疏水键机制对土土样表面进行修饰的临界点；揭示了有机修饰改性土对有机物、重金属吸附过程的热力学特征，填补了该项研究工作的空白；阐明了修饰剂在改性土表面呈分散态的非均匀分布，改性土表面具有不同性质表面共存的表面特征和对污染物多机制共存的吸附特征，确定了阳离子表面修饰剂在土表面的修饰是一种离子交换和疏水键结合两种机制相伴并行且为非等电荷量交换的修饰反应模式，以及土具有对CTMAB的吸附偏好性远大于对钙离子吸附偏好性的特征。

On the basis of which, the grade of coarse aggregates is confirmed. The experimental results are validated through the theory calculation.(2)The reasonable ratio of coarse aggregates to fine aggregates is confirmed by the mathematics mold method and interrupted aggregate gradating experiment.

并以此为基础，确定了粗集料的级配，使振实后的粗集料能够达到最大的密实度，同时应用理论计算对试验结果进行验证通过振实试验和容重方法研究了不同粒径粗集料混合后的空隙率。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Consider that processes the factor such as being convenient, the whole frame adopts the stencil plate to press the shaping craft, so as to ensure the whole intensity of the frame; The main body of the frame has continued to use the traditional ladder-shaped structure, of simple structure, stability is good; Assign the scheme wholly according to the electric automobile, has confirmed the physical dimension of the frame; Combine the scheme of decorating of the main spare part of electric automobile, has confirmed the frame is released, the crossbeam one decorates a form and connects the method, install the needing of main spare parts such as the gear box,etc. in because some crossbeams, has taken the structural form of the ingot roof beam.

考虑到加工方便等因素，整个车架采用钢板冲压成型工艺，以保证车架的整体强度；车架主体沿用了传统的梯形结构，结构简单，稳定性好；根据电动汽车整体布置方案，确定了车架的结构尺寸；结合电动汽车主要零部件的布置方案，确定了车架纵、横梁的布置形式及连接方法，部分横梁因为安装变速器等主要零部件的需要，采取了元宝梁的结构形式。

But GST= 0.156, Nm=1. 588. As a result, the foundation of Youyongchi Avicennia marina population was the result of the migration of hypocotyles and human factors.

这项工作可以为海岸防护林中新引进种类的判定以及为研究种群建立者效应方法的确定提供科学依据。

The two-dimensional CDRC-ADI-FDTD update equations for collision unmagnetized plasma are induced. The unconditional stability of the CDRC-ADI-FDTD formulation for collision unmagnetized plasma is obtained by the examples.

推导了碰撞非磁化等离子体中的二维CDRC-ADI-FDTD迭代公式，并用算例验证了碰撞非磁化等离子体CDRC-ADI-FDTD算法也是无条件稳定的。