查询词典 confirmed

Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.

结果(1)成功构建人大细胞肺癌高低转移株差异表达基因正向消减cDNA文库和反向消减cDNA文库；(2)经蓝白菌落筛选和斑点杂交，正向消减文库获得307个阳性克隆，反向消减文库获得78个阳性克隆；(3)正向消减文库挑选55个克隆成功测序，经同源性分析最终确定差异表达基因片段23个与已知人类全长基因具有很高相似性(95％～100％)，这些基因包括NME2、NPM1、MT2A、HSPE1、TAFlA、EPRS、PX19和EIF3S9等；(4)反向消减文库挑选31个克隆成功测序，经同源性检索和比对最终确定差异表达基因片段16个，其中15个与人类全长基因具有很高相似性(95％～100％)，包括ANXA2、TUBB、PKN2、GNAS、EEF1A1、SSR2和RPLPO等基因。1个片段和己知人类基因仅有部分相似性，表明它可能是未被克隆的人类基因EST片段。

In the method of time-frequency the phase of unbalanced force is confirmed by analysis of time domain and amplitude of unbalanced force is confirmed by analysis of frequency.

在这种时-频动平衡方法中，用时域分析来确定不平衡力的相位，用频域分析来确定不平衡力的大小。

Jake confirmed that he had personally seen some of the classified maps showing dramatically altered future coastlines, and also confirmed the possibility of a very advanced high-speed 'shuttle-like' system that connected many places, like the US and Australia under the Pacific Ocean - a longstanding but always uncorroborated rumor within the UFO community that had acquired semi-mythological status since the startling reports of John Lear and William Cooper in the late 1980s.

杰克证实，他曾亲自见过一些机密地图，上面显示了引人注目的、改变了的未来海岸线，同时他确证，未来可能会出现非常先进的高速运转的"梭子般的"（shuttle-like）系统，来连接许多地方，就像美国和太平洋下面的澳洲-这是一个长期存在的，但却总是未被证实的、流传在UFO社区里的传闻

Syphilis was confirmed by clinical and serum test of TPHA and RPR; That of pemphigus vulgarism was confirmed not only by clinical, histopathological examination, but also by direct and indirect immunofluorescence test.

检测部分皮肤病结合珠蛋白的表型分布，探讨结合珠蛋白的表型与部分皮肤病的关系及意义。

Sample collection: Two hundred and fifteen sera samples of normal population were collected from the peripheral blood of healthy blood donors of Shenyang, and the sera were separated by centrifugation after blood clotting; The patients sera were collected from the outpatients and inpatients of No. 1 hospital of China Medical University and the Second hospital of Jilin University. Syphilis was confirmed by clinical and serum test of TPHA and RPR; That of pemphigus vulgarism was confirmed not only by clinical, histopathological examination, but also by direct and indirect immunofluorescence test. DIF test on lesion or non-lesion skin showed immunoglobin and/or C3 deposits in the intercellular space of epidermis, and IIF test showed positive anti- intercellular antibody in patient serum using mouse's or rat's tongue or esophagus as substrate.

标本的收集：正常人的血清：采集沈阳市正常献血员外周静脉血共215份，待血凝后离心分离血清；患者的血清：来自中国医科大学一院及吉林大学二院门诊及住院部的病人，梅毒患者除了病史及临床表现外，全部经血清学TPHA及RPR确认；寻常性天疱疮患者除了临床及组织病理诊断外，还进行了直间接免疫荧光的检测，直接免诊断疫荧光：在皮损区及非皮损区的表皮细胞间显示IgG和/或C3补体沉积；间接免疫荧光用鼠或兔的舌或食管作为底物，在病人的血清中显示有肯定的细胞间抗体。

Asked the Duck, who came paddling up some minutes afterwards.'He has a great many good points, but for my own part I have a mother's feelings, and I can never look at a confirmed bachelor without the tears coming into my eyes.''And how do you like the Water-rat?' asked the Duck, who came paddling up some minutes afterwards.'He has a great many good points, but for my own part I have a mother's feelings, and I can never look at a confirmed bachelor without the tears coming into my eyes.

鸭太太在水里划了几分钟后，才上岸来问道。who came paddling up some minutes afterwards（顺序上是先在水里有了会儿，然后上来问，可以说"鸭太太划了几分钟后上来问"）"他还是有很多优点的，不过从我个人的角度来说，我总有一副做母亲的心肠，一旦看到铁了心不肯结婚的单身汉我总会忍不住掉眼泪。"

The structures of three key active constituents are confirmed, and the structure of one main impurity β-caryophyllene is also confirmed; the wrong assignment of 13C-NMR of β-caryophyllene was firstly rectified.

不仅对榄香烯原料药的3个主要活性成分进行了结构确证，而且对原料药中的1个主要杂质成分β-石竹烯进行了结构确证，并首次对文献中β-石竹烯碳谱数据中有误的归属进行了纠正。

Lines which were 2n=21"+1" and one line which was 2n=20"+2", one line which was 2n=43. The salt-resistance addition line Line15 was confirmed as a Disomic alien addition line by C-banding, SSR, FISH methods, and the two alien addition chromosomes had close correlation with the long arm of the second homoeologous group, the centromere region of the third homoeologous group and the short arm of the seventh homoeologous group. We also confirmed the particular C-banding type of the alien addition chromosomes by C-banding following FISH method. The drought-resistance addition line Line17 was found also as a Disomic alien addition line by SSR and FISH detection. The alien addition chromosomes had close correlation with the short arm of the second homoeologous group.

对导入赖草优质耐盐基因的株系Line15进行了C-分带、荧光原位杂交、SSR相结合的鉴定，确认Line15是一个二体异附加系，附加的一对染色体与小麦的第二同源群长臂、第三同源群着丝粒和第七同源群短臂密切相关，并确定了Line15的外源染色体C-分带特征带型，为目后鉴定Line15附加系的真伪提供了简便快捷的途径；对抗旱附加系Line17进行了荧光原位杂交和SSR检测，得出Line17也是一个二体异附加系，外源染色体与小麦的第四同源群短臂密切相关。

Classical electric mechanics confirmed that accelerating charge must eradiate electromagnetic wave, while at the same time, quantum theory confirmed electron accelerates in the same energy-interzone in atom never radiate electromagnetic wave, is it possible to be united in theory?

经典电动力学认为加速运动的电荷能够辐射电磁波，而量子力学指出电子在同一能级内做加速运动不能辐射电磁波，能否把它们统一起来？

Results The expression plasmid of pVAX1 IL-18 HN was contructed successfully confirmed by enzyme digestion identification. The expressions of IL-18 and HN chimeric genes were confirmed, and the expressed HN protein had higher hemagglutinative titer.

结果：酶切鉴定证实正确地构建了pVAX1 IL-18 HN嵌合表达质粒，Western blotting和血凝试验检测结果表明，嵌合后的IL-18和HN基因均能够表达，且表达的HN蛋白具有较高的血凝活性。

I was just taking her hand, to help her out of a car, and I knew it.

我只是握着她的手，扶她下车，但我知道这一切。

China and South Korea have excoriated Japan over its approval of new school books which they say whitewash the atrocities committed during Japanese occupation, and anti-Japanese protests have been held in Chinese cities. The lingering bitterness over Japan's past imperialism still threatens to mar relations between the big East Asian powers

中国和韩国强烈谴责日本通过新的中学历史教科书，他们认为这一教科书掩盖了日本在其占领期间所犯下的残酷暴行，反日抗议活动在中国数座城市发生，历史上日本帝国主义制造的痛楚无法被忘却，这有可能破坏东亚强国间的关系。