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Methods: Two recombinant palter plasmid containing whole CDNA sequences of HMCD59 transfected CHO cells by lipofectamine with PCDNA respectively. Then G418 was used to select positive clone.

分别构建两种含有HMCD59全长CDNA序列的重组pALTER质粒，并与pCDNA3质粒，按3：1比例混合后，运用脂质体介导法共转染CHO细胞(编号为HM5-CHO及HM6-CHO)，用新霉素类似物G418初步筛选出阳性克隆。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

For the first time, this paper presented the concepts about two basic deformations of Pascal's triangle, that is, Shifted Deformation and Clone Deformation.

本文首次提出了杨辉三角的两种基本变体的概念，即错位变体和克隆变体。

Segments of Adh gene in azuki bean (variety KS 5), covering an interval between partial intron 3 and intron 9, were amplified and sequenced with two pairs of primers. The result has proven the viability of using conserved sequences in a completely sequenced gene of one species to clone same gene in another species. In the comparison between Adh genes of azuki bean and pea, high sequence similarity (about 80%) in exon and long length difference (up to 116 bp) in intron were found. Besides, the edge regions of Adh introns with GT/AG counterparts and more conserved sequences than central regions was observed in both species.

利用两组引子对完成红豆(高雄5号)Adh基因之部分解序，其范围包括部分intron 3至intron 9之间的区域，也印证利用基因之已知保守序列可选殖另一物种之相同基因，红豆与豌豆之Adh基因序列比对结果显示两者之exon均较intron有较高之GC百分比(分别为41.0 ~ 48.7%及23.3 ~ 35.2%)，且两者在exon区域之DNA序列相似程度约80 %，而在intron区域中则有明显的序列差异存在(长度差异最高可达116 bp)，但是intron外侧序列均具有GT/AG组成且相对较中央序列具有保守性。

Specific siRNA was designed and synthesized, and transfected Hela with liposome siNEO FX, then analyse the role of siRNA on proliferation vitality outside the Hela cells, cells periodic distribution by MTT method, double-layer soft agar clone-forming assay, and flowing cell technology.

设计并合成siRNA，脂质体siNEO FX转染Hela细胞，分别用MTT法，双层软琼脂克隆形成试验和流式细胞术分析了siRNA对Hela细胞体外生长增殖活力、细胞周期分布的作用。siRNA作用后的Hela细胞增殖速度减慢，软琼脂克隆形成率降低，G0/G1，期细胞比率增加。

1Ml. After the selection of phagocyte, we gain the rarefied virus clone.

病毒分离自一病死仔猪的小脑、脑干和延髓等组织。

The further identification indicated that free BmVUB could block the combination of 10 positive phagocyte clone with solid BmVUB.

对这10个阳性噬菌体克隆进一步鉴定，游离BmVUB可阻断10个阳性噬菌体克隆与固相化BmVUB结合。

Clone R46 was not clustered into any known phyla and might belong to a novel taxonomic group.

本研究还发现了分类地位尚未明确的一菌群（R46）。

The cDNA clone encoding a planarian SPARC (DjSPARC-like) protein was isolated from a cDNA library of Dugesia japonica.

从东亚三角涡虫文库中分离的克隆N362，DjSparc-like基因的cDNA编码的最大开放阅读框为783bp，由261个氨基酸组成。

Rufa Planch could restrain the cell proliferation in the stomach carcinoma SGC7901, lymphocytic leukemia L1210, mouse neuroblastomax rat gliom hybrid NG108-15, and human hepatocellular carcinoma Bele7404 cell strain by clone cells and restrain the cell SGC7901, NG108-15 cell strain by MTT.

结果山梨猕猴桃根提取物用集落形成法R6，R8对胃癌细胞株SGC7901、白血病细胞株L1210、神经性肿瘤细胞株NG108-15、肝癌细胞株Bele7404的增殖有抑制作用；用MTT法对NG108-15，SGC7901的增殖有抑制作用。

Panorama Many people are dancing in a large sitting room.

在一个大客厅里，许多人在跳舞。

We fully understand how a microwave oven cooks food, it is first necessary to talk ...

我们充分理解一个微波炉煮的食物，这是首先要谈。。。