查询词典 bud mutation

Compared with other HPV16 reference sequence, nucleotide sequencinganalysis of the HPV16 E6, E7 and L1 gene from Cervical Carcinoma Biopsis in Chengdu city and Chongqing of China consistently shows point mutations,including synonymous mutation and nonsynonymous mutation. This researchwill be helpful in preventing cervical cancer and designing vaccines which isspecific for the women in the two regions of China.

与标准序列比较，中国成渝地区HPV16亚型L1、E6和E7基因存在一定范围的变异，提示我们，在预防治疗成渝地区妇女宫颈癌病人以及研制开发针对成渝地区预防宫颈癌的HPV疫苗时，需要注意这些位点的变异，特别是一些引起氨基酸变异的位点。

The otological, audiological and neurological examinations of these patients were conducted, including otoscopy, pure-tone (Madsen 522) and immittance(GSI 33) audiometry. MtDNA A1555G mutation and GJB2 235delC mutation were detected with specific restriction enzyme digestion methods.

对 148 名非综合征型感音神经性耳聋患者分别进行线粒体 DNA 12SrRNA基因A1555G 点突变和 GJB2 基因235delC突变的限制性内切酶分析。

To verify pathopoiesis of PRKAG2 with a novel missense mutation G100S responsible for Chinese PRKAG2 cardiac syndrome and promote pathogenic investigation of the PRKAG2 gene,we study the functional consequences of the mutation in the level of the cell and molecule by overexpressing mutants of PRKAG2 in CCL13 cells.

为了验证PRKAG2 G100S新突变在中国人PRKAG2心脏综合征家系中的致病作用，深入认识PRKAG2 G100S错义突变引起的功能变化，推动PRKAG2基因的致病机制研究，我们通过在CCL13细胞中过表达突变基因，从细胞、分子水平研究了PRKAG2 G100S突变的生物学功能。

The experiment shows that human pancreatic cancer cell line Patu 8988 has GGT→GTT mutation at codon 12. The method of PCR-SSP is rapid, convenient, economical and high specific for detecting K-ras gene point mutation. The goal in the long term is to develop a simple and useful clinical procedure for early diagnosis and differential diagnosis of pancreatic cancer.

明确了人胰腺癌细胞株Patu 8988 K-ras基因第12位密码子点突变方式，为胰腺癌的下一步基因治疗研究打下了坚实的基础；PCR-SSP方法检测K-ras基因点突变简便、快速、经济且特异性高，有望在临床上成为早期诊断和鉴别诊断胰腺癌的实用检测方法。

Results The results of PCR-SSP and sequence analysis showed that the Patu 8988 cells had point mutation at codon 12 and the mutation style was GGT→GTr.

体内实验表明K-ras mutation ASODN较SODN组、RODN组和对照组能有效抑制BALB／C裸鼠人胰腺癌的生长（P〈0.01）。

Results The human pancreatic cancer cell line Patu 8988 had point mutation at codon 12 and the mutation style was GGT→GTT. The result of PCR-SSP was completely in accord with that of sequence analysis.

结果 PCR-SSP法检测人胰腺癌细胞株Patu 8988存在K-ras基因点突变，突变方式为GGT→GTT，其结果与基因序列测定完全相符。

Methods Three kinds of special sequence primers for polymerase chain reaction with regard to the mutation styles (CGT、GTT、GAT) at codon 12 of K-ras were used to study the human pancreatic cancer cell line Patu 8988.The amplification products were studied with 12% polyacrylamine gel eletrophoresis to detect the style of point mutation.

针对K-ras基因第12位密码子点突变方式(CGT、GTT、GAT)设计顺序特异性引物，对人胰腺癌细胞株Patu 8988进行聚合酶链反应，扩增产物经12%非变性聚丙烯酰胺凝胶电泳后判断该细胞株有无K-ras基因点突变及其突变方式。

Chapter five looks at the mutation in language stream with contemporary philologic theories. The characteristics of mutation in changshan dialect are supposed to be show by tone changing , light syllable, r-suffixation, z-suffixation,etc.我每is possibly the r-suffixation of women我们;Some final varied pattern of suffix子is actually r-suffixation;Changshan dialect"s light syllable lies in every level of language and it has multifold forms; There is rich tone change of verbs which can present certain grammatical meanings in Changshan"s dialect.

第五章动态的描写了长山方言中的语流音变，利用当代语音、语法、语义理论从轻声变调、语法变调、小称变韵、子变韵等几个方面考察长山方言语流音变的特点，认为&我每&有可能是&我们&的小称形式；以闻喜方言为代表的&子变韵&并不是&子&音转变而来，而是&儿化&的一种形式；长山方言的轻声变调存在于词汇、短语、句子等多个语言层面，有三种表现形式；轻声助词&了&、&着&、&到&省略后，会引起前面动词的语法变调，等等。

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法：利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变，使编码子249由精氨酸变成丝氨酸，然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中，将注射过的囊胚植入假孕的雌性小鼠子宫，到第14d取小鼠胚胎纤维母细胞，用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞，经测序证实细胞含有由249Arg到Ser的突变。

Missense mutation of Tyr486Asp in Exon 5 of UGT1A1 gene is the pathogenic factor of the CNⅡ case in the family and recessively inherited. The mutation has no influence on the response elements of phenibarbital in the promoter region.

Tyr486Asp突变是本例CN-Ⅱ家系的致病性基因，该突变不影响UGT1A1基因启动子区苯巴比妥酸的应答元件，其遗传规律符合常染色体隐性遗传。

The two-acre Dunbar Community Garden in Little Rock has served as a model for several years.

几年来，坐落于小石城，占地两英亩的邓巴社区菜园树立了一个标杆。

Jerry points to the jacket hanging in the bathroom. It's ruined, and unwearable What?

杰瑞指着洗手间里挂着的夹克，毁了，不能穿了）什么？