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CTAB was used to pyrolyse plant cell and reacted with the released cell nucleic acid to form nucleic acid-CTAB complex, the cell extract was then deproteined with chloroform-isoamylol, at last RNA was selectively precipitated by LiCl.

采用CTAB裂解植物材料，与细胞中的核酸形成核酸-CTAB复合体，然后用氯仿一异戊醇去除细胞裂解液中的蛋白，最后采用LiCl选择性沉淀其中的RNA。

CTAB was used to pyrolyse plant cell and reacted with the released cell nucleic acid to form nucleic acid-CTAB complex,the cell extract was then deproteined with chloroform-isoamylol,at last RNA was selectively precipitated by LiCl.

但是一般高等植物组织内都含有多糖类物质，这些物质的存在严重影响RNA的提取，如果去除不净，将影响反转录、扩增等下游操作。

To understand the infectivity by porcine endogenous retrovirus with porcine skin fibroblast cell in vitro and in vivo, porcine skin fibroblast cell established by our laboratory were co-cultured with neo/HEK293 cell for the infection of RERV in vitro, and were subcutaneously transplantated to SCID (severe combined immuno-deficiency) mice for the infection of PERV in vivo, laying the foundation for valuation of biologic safety of xenotrans-plantation. The event of neo/HEK293 cells infected by PERV occurred during co-culture of porcine skin fibroblast cells with neo/HEK293 cells, expanding the rang of the infection of porcine endogenous retrovirus. Afterpig cells transplantated subcutaneously in SCID mice, the microchimerism (78.57%) of pig cells occurred widel, and there was phenomena of integration of PERV provirus (85.71%) in several organs or tissues remote from the injected sites, indicating infection of PERV in SCID mice in vivo. yet, there is no evidence of active viral replication in analysis of PERV env RNA of these tissues or organs.

为了解猪皮肤成纤维细胞PERV在体外和体内的感染性，通过建立猪皮肤成纤维细胞系，将所建细胞系与人胚胎肾293细胞体外共培养，并移植于严重联合免疫缺陷鼠皮下进行猪皮肤成纤维细胞PERV的体外和体内感染性实验，结果表明，猪皮肤成纤维细胞与人胚胎肾细胞共培养过程中，猪内源性逆转录病霉感染人胚胎肾细胞，进一步证实和拓宽了猪细胞PERV感染人细胞的范畴；猪皮肤成纤维细胞移植SCID鼠皮下后，导致SCID鼠发生猪细胞微嵌合(78.57%)和PERV在体内感染(85.71%)并且波及远离移植部位的多种组织或器官，但是并未检测出SCID鼠组织中表达PERV env RNA。

A number of lines evidence indicate that calcium treatments maintain the structure and function of the cell walls, increase the fruit firmness, decrease the activity of the cell wall degration enzymes and ethylene production, maintain the integrity and stability of the membrane, activate some oxidases, induce phytolexin and regulate cell function as a signal mobecular.

钙的防腐机制在于对果蔬采后腐烂的控制，保持细胞壁的结构与功能，增强果实硬度，降低细胞壁降解酶的活性，抑制呼吸，降低乙烯生成量，保持细胞膜的完整性，激活体内的某些氧化酶，诱导合成植保素，作为信号分子，调节细胞功能。

It has beenconfirmed that the occurrence of basal cell carcinoma, small cell lung cancer,pancreatic cancer, prickle cell carcinoma, prostatic carcinoma, carcinoma of bladder,medulloblastoma and gastric cancer of mankind have a close relationship with the abnormal activation of Sonic hedgehog signaling pathway.

现已证实人类皮肤基底细胞癌、小细胞肺癌、胰腺癌、口腔鳞状细胞癌、前列腺癌、膀胱癌、髓母细胞瘤、胃癌等肿瘤的发生与sonic hedgehog信号通路异常激活密切相关。

According to the structure of wood cell wall, the preparation of aerogel-type wood mainly includes three steps, namely the destruction of wood cell wall in S3 layer, the expansion of wood cell wall and supercritical drying.

根据木材细胞壁的结构，气凝胶型木材的制备主要包括3个步骤：木材细胞壁S3层的破坏；木材细胞壁的膨化；超临界干燥。

The cell outline was collected with two-step expansion model of modified Snake,at the same time;greedy algorithm was applied to reduce the operative time of procedure.Upon the basis that the segmentation of wood cell intracavity and edge was finished,wood cell wall was identified.

利用改进的Snake两步膨胀模型对细胞轮廓进行提取，同时应用贪婪优化算法，减少程序运行时间，在完成对木材细胞内腔和边缘分割的基础上，完成对木材细胞壁的识别。

Different cells,cell density and cell shape lead to comparatively great difference in wood properties and physical mechanical properties, change of wood cells affects change of wood properties including wood quality,wood property and all other aspects,and wood properties decide economic value and directly affect manufacturing and utilization of wood,therefore,the research and analysis on wood cell structure become especially important.

由于树木生长不均匀，使各种树木的木材构造具有多样性，不同细胞体、细胞密度和细胞形状导致了木材材性和物理力学性能存在较大差异，木材细胞的变化影响着木材性质的变化，木材的性质又决定木材经济价值，并直接影响木材的加工和利用，由此对木材细胞结构分析研究就变得尤为重要。

Based on setting up mathematical model of wood fiber cell structure, this paper studied on the variation description method of wood fiber cell structure, at the same time, this item set up the judging rule of the smallest cutting thickness of micrometer wood fiber cell splitting, gaining the high strength MFB of poplar whose modulus reach above 5171MPa.

本文在建立木纤维细胞结构数学模型的基础上，进行了木材纤维细胞结构劈裂描述方法的研究，建立了微米木纤维细胞劈裂的最小切削厚度判定原则，MFB的实验研究获得了弹性模量达到5171MPa以上的高强度试件。

During two-celled pollen grain stage, they were accumulated on the wall of the generative cell. After the generative cell came into the vegetative cell, ppts largely disappeared.

二胞花粉刚形成的生殖细胞表面呈现大量的ATPase反应颗粒，当生殖细胞脱离花粉内壁移入营养细胞，ATPase反应颗粒基本消失。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。