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After renal cortexes were Grinded and digested with 0.25%tryspase.The cell types of epithelial cell were identified by immunocytochemistry.The injury model of rats, renal tubular epithelial cells were established by using gentamicin.And observed the role of bFGF in renal tubular epithelial cell.

通过对大鼠肾皮质研磨、过网的方法，并以0.25%胰蛋白酶消化，以免疫细胞化学方法鉴定体外培养的肾小管上皮细胞(抗Cytokeratin 18抗体)，以庆大霉素对培养的肾小管上皮细胞建立损害作用模型，然后观察碱性成纤维细胞生长因子对损害的肾小管上皮细胞的保护作用。

Methodshexamethyldisiloxane polymer film was obtained by plasma polymerization method on coverslips. oxygen (o2) plasma treatment was employed to modify the surfaces with different time so that a series of surface with different wettability were produced. l929 cell were cultured on these surfaces to observe the cell attachment rate and cell spreading in 6 and 12 hours.

使用薄膜等离子体聚合法在圆形盖玻片表面生成六甲基二硅氧烷(hexamethyldisiloxane， hmdso)薄膜聚合体，然后用低温氧等离子体轰击聚合体表面，根据轰击时间的不同形成一系列的不同润湿性表面，将成纤维细胞系l929接种于不同润湿性表面，观察6h、12h时细胞的贴附率，并观察细胞的形态。

Methods Hexamethyldisiloxane polymer film was obtained by plasma polymerization method on coverslips. Oxygen (O2) plasma treatment was employed to modify the surfaces with different time so that a series of surface with different wettability were produced. L929 cell were cultured on these surfaces to observe the cell attachment rate and cell spreading in 6 and 12 hours.

使用薄膜等离子体聚合法在圆形盖玻片表面生成六甲基二硅氧烷(Hexamethyldisiloxane， HMDSO)薄膜聚合体，然后用低温氧等离子体轰击聚合体表面，根据轰击时间的不同形成一系列的不同润湿性表面，将成纤维细胞系L929接种于不同润湿性表面，观察6h、12h时细胞的贴附率，并观察细胞的形态。

Methods Human monocyte cell line THP-1 was cultured and induced to macrophage by PMA treatment. The effect of 9-cisRA on the differentiation induced by PMA was studied. Cell morphology was observed by phase contrast microscope and the adhesion rate of THP-1 was detected by methyl thiazolyltetrazolium assay. The cell surface markers involved in differentiation of mono cyte/macmphage (CD11b, CD36) were analyzed by flow cytometry.

体外培养人单核细胞系THP-1，经佛波酯诱导分化为巨噬细胞，同时给予9-顺式维甲酸对佛波酯诱导分化进行干预，通过相差显微镜观察细胞形态，MTT比色法检测细胞贴壁率，流式细胞仪检测与单核／巨噬细胞分化有关的细胞表面标志物CD11b和CD36的表达。

Concludes:Three subsets of mast cell in human tissue are sorted and depurated with antibodies of specific enzyme and immunofluorescence labelling and flow cytometry. Mast cell of containing profuse secretary vacuoles could be displayed by confocal microscopy. It demonstrate that mast cell have material substructure offering for fast reaction of I type allergies of human being.

利用肥大细胞的特征性酶抗体、免疫荧光标记和流式细胞仪可将人组织中的肥大细胞分选纯化为三种亚型；以共聚焦显微镜显示肥大细胞含有丰富的分泌颗粒，它说明肥大细胞具备了为人体I型变态反应提供快速反应的物质基础。

When the concentration of lead was very high, the most of lead linked weekly with the cell wall of root and was easy to dissolve into acidic solution. But about 50μg Pb/g cell wall was very hardly desorbed, it might be entered into the cell wall. This phenomena showed that the complete desorption of lead will be very difficult.

铅浓度高的细胞壁，其相当大部分的铅与细胞壁的结合力并不大，因此在用强酸处理时，就比较容易地溶解下来，但是大概有50微克／克细胞壁的铅，它们的结合牢度大，或者深入到了细胞壁的组织中，因此很难解吸下来，这将会对铅的彻底去除带来困难。

The viscosity range between the inside of the cell to the outside of the cell is about 36 dynes per cm2 depending upon the cell hydration and other factors.

细胞内和细胞外的粘滞度范围约为每平方厘米36达因，这取决于细胞水化状态和其它因素。

ResultsMTT assay showed that various concentration of VM26 could effetely inhibit the growth cell; according to the results of flow cytometry, some typical sub diploid peaks were observed,the distribution of cell cycle phase changed at various time, the cell cycle of G2/M phase was arrested within 48 hours.

结果在一定浓度范围内，细胞的生长抑制率随着浓度的增加而增加，细胞的凋亡率随着时间的增加而增加，细胞周期被阻滞在G2/M期，在48小时达到高峰。

Results of the experimental groups: Comparison with the control groups, in 6h and 8h groups eminences of ependymal cells with cobblestone-like appearance were seen, their microvilli were further richer, and a number of microholes could be found on their base. In body part of SFO cell membrane of some ependymal cells were broken completely, their cytoplasm was no longer seen, and cell nucleus located at one side of the cell, 5-7μm in diameter and dividing lobe-like in shape. Beside those cells some ependymal cells appeared many folds on the top of surface. In 2h and 16h groups the observation results seemed no significant differences from the corresponding control groups.

实验组结果：与对照组相比，在6小时和8小时实验组中，可见成团的鹅卵石状室管细胞隆起于表面，微绒毛更为丰富，微绒毛根部可见到若干微细小孔，在SFO体部的一些室管膜细胞的胞膜完全破裂，胞质几乎荡然无存，仅剩胞核居于细胞的一侧，直径有5.7μm，呈分裂叶状；与这些细胞邻近的室管膜细胞表面最高处多呈现皱襞。2小时与16小时的实验组的观察结果与对照组相比未发现明显差异。

The effect of AA on BEL-7402 was not significant; However, AA could effectively enhance AT-induced hepatocarcinoma cell apoptosis and lesion severity through activation of caspase-3 but not ERKs-Conclusion; Caspase-3 and ERKs proteins could involve in arsenic-induced hepatocarcinoma cell apoptosis and differentiation respectively as intracellular signaling molecules; The effect between AT and AA on hepatocarcinoma is synergistic, which further inhibits cell growth and induces apoptosis in human hepatocarcinoma cells through activation of caspase-3 but not ERRs.

AA对BEL-7402无明显的作用，但能有效的通过caspase-3而不是ERKs的激活促进AT对肝癌细胞的凋亡效应。结论：ERKs和caspse-3信号蛋白可能分别参与了砷剂诱导的人肝癌细胞促分化和凋亡效应，AT和AA对肝癌细胞的作用有协同性，他们通过caaspase-3而不是ERKs的激活进一步抑制了肝癌的生长，诱导其凋亡。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。