查询词典 amplified

When C.farreri×P.yessoensiswere tested by using6pairs of microsatellite primers which could be applied to boththe two kinds of scallops,the specific bands could be amplified fromeachone of the 6 pairs,among which,the specific bands fromboth agnate and maternal colony could be distinguished obviouslyinthe amplified products of P13F449 and KMY134,and accordinglythe hybrid identity of hybrid generations could be affirmed.

将这6对具有属间通用性的微卫星引物对栉孔扇贝×虾夷扇贝进行PCR扩增，均能扩增出特异性条带，其中引物P13F449和KMY134的扩增产物中可以明显地分辨出来自父母本群体的特有条带，从而确定了杂交子代的杂种身份。

The fragment size can be amplified by 295 bp through the primer, in order to prevent false positive from appearing, a pair of internal control primers C34 is designed through the invention according to a bull autosome 3 reported sequence, the fragment size is amplified by 208bp, dual PCR amplification is performed to single bull sperm through the two pairs of primers, and then the final evaluation is performed to the sperm separation purity according to the statistical analysis to the detection result.

本发明提供了一种检测X、Y精子分离纯度的引物，该引物是针对牛Y染色体上性别决定基因Sry通过PCR错配技术设计而成，通过该引物可扩增片段大小为295bp，为了防止假阳性出现，本发明根据牛3号常染色体报道序列设计了一对内标引物C34，扩增片段大小为208bp，通过上述两对引物对单个牛精子进行双重PCR扩增，然后根据对检测结果的统计分析，对分离精子纯度做出最终评价。

There were 8 embryos single blastomere amplified, and the amplification success rate was 72. 7%(8/11); 3 embryos' not amplified; 2 embryos'showed preferential amplification , and the PA rate was 25%(2/8); and the 7 tubes of blank with washing cell solution were not contaminated. We transferred 3 unaffected embryos to the uterus. But unfortunately, blood hCG was negative after 2 weeks of transferring indicating no pregnancy.

共8枚胚胎的单卵裂球得到扩增，扩增成功率为72.7%（8/11），3枚未得到扩增，2枚发生了偏性扩增(preferentialamplification，PA)，PA的发生率为25%（2/8），细胞洗脱液空白对照7管均无污染发生。3枚诊断为正常的胚胎被移植入宫内，不幸的是，移植后2周，血hCG阴性，证明未妊娠。

The result indicated that 237 combinations amplified a total of 13 079 AFLP fragments with an average of 55 fragments, the average of 1.30 markers per 1 000 AFLP fragments were found to be linked to clubroot resistant gene; the amplified polymorphic fragments were 3 167 between the two parents, account for 24 percent of polymorphic frequency; the number of amplified fragments and the number of polymorphic fragments was in positive linear correlation; GC content of AFLP primer combinations significantly affects the number of amplified fragments and the number of polymorphic fragments, indicating negative linear correlation between them.

结果表明：平均每个组合检测到55条带，共扩增13079条带，平均每1000条带检测到1.30个根肿病抗性基因连锁标记；多态性带数为3167条，双亲间多态性频率约为0.24；扩增带数与多态性带数呈线性正相关，引物组合中的不同GC含量与扩增带数和多态性带数存在显著性差异，并表现出线性负相关。

In this paper, Amplified fragment length polymorphisms markers were used to explore genetic variation and divergence among two selected lines and one cultured control of the pearl oyster, Pinctada martensii Dunker.

AFLP (Amplified fragment length polymorphism)是一项基于 RFLP 和 RAPD 基础上的分子标记技术，广泛应用于动植物和海洋生物的种质资源鉴定及群体遗传结构分析中。

Some intergeneric hybrids (Sugarcane×Erianthus fulvus) were identified by chromosome counting and RAPD (Random Amplified Polymorphism DNA) technique.

采用体细胞染色体计数和RAPD(Random Amplified Polymorphism DNA)分子标记的方法鉴定甘蔗与蔗茅属间杂交种F1的真实性。

To validate an investigation method for the epidemiology of Clostridium perfringens in chicken,genetic diversities of 34 strains of C.perfringens type A isolated from healthy chicken of 10 diffe-rent integrated chicken farms in Sichuan Province were analyzed by repetitive extragenic palindromic PCR and amplified fragment length polymorphism methods.

为探讨鸡产气荚膜梭菌流行现状的调查方法，采用REP-PCR(repetitive extragenic palindromic PCR)技术对四川省10个规模化鸡场分离得到的34株A型产气荚膜梭菌进行了遗传多样性分析，并与AFLP（amplified fragment length polymorphism）方法进行了分型比较。

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

Methods The genomic DNA extracted from the 2 trematodes was amplified with random amplified polymorphic DNA and then was carried on agarose gel electrophoresis.

应用随机扩增多态性DNA（Random amplified polymorphic DNA，RAPD）技术对斯氏肺吸虫和华支睾吸虫的基因组多态DNA进行扩增，扩增产物经琼脂糖凝胶电泳后分析结果。

The typical spot symptoms on fruit would decrease the production and merchandise of tomato in the heavy rain of June and July; therefore, the key period for disease control is after rainy season. Quick identification on pathogen was using PCR. 1476bp band was amplified by primer P3、P4of bacterial speck. By using primer CMM5、CMM6 for bacterial canker, 614bp fragment was amplified. These PCR tests provided important foundation for pathogen identification and disease diagnoses. Using primer RST65 and RST69 for bacterial spot, expected 420bp band was not amplified.

通过聚合酶链式反应对病原进行快速鉴定，证明加工番茄细菌性斑点病用引物P3、P4可以得到一条大小为1476bp的特异性条带，番茄细菌性溃疡用CMM5、CMM6可以扩增出一条大小为614bp的特异性条带，这为病害的快速诊断打下了基础，但利用引物RST65和RST69对番茄细菌性疮痂病进行鉴定，没有得到预期420bp的目标条带，故对细菌性疮痂病的快速检测还有待继续研究。

Graf, without question, is the greatest women's player in history.

格拉芙，现在，毫无疑问是女子网坛历史上最伟大的选手。

If not, you will have to look around at all your important data.

如果没有，您将需要检查所有的重要数据。