查询词典 amplified

Methods The 9 STR loci (D11S2368, D12S391, D13S325, D18S1364, D22-GATA198B05, D6S1043, D2S1772, D7S3048, D8S1132) were amplified with STR_Typer_10_v1 kit for 1619 unrelated individuals of Han population in Southern China. The PCR products were analyzed with 3100 genetic analyzer and GeneMapper ID 3.1v software. The forensic efficiency parameters were calculated by PowerState V12. xls and the Hardy-Weinberg equilibrium was tested with Arlequin 3.11v software. The genetic polymorphism of 9 STR loci in Han population of Southern China was quite high.

用STRT_yper_10_v1荧光标记试剂盒，对1619例中国南方汉族无关个体的9个STR基因座(D11S2368， D12S391， D13S325 D18S1364， D22-GATA198B05， D6S1043， D2S1772， D7S3048， D8S1132)进行扩增，用AB公司3100遗传分析仪和GeneMapper 3.1v软件作STR分型，用PowerStateV12.xls分析软件进行等位基因频率和法医学常用参数统计分析，用Arlequin 3.11v软件包作Hardy-Weinberg equilibrium平衡检验。

All the probes were pointed on glass slides by GMS 417 Arrayer. Amplified plasmids wer.e labeled by PCR fluorescence(CY5)-primer and CY5-dUTP. The labeled plasmids were applied to the DNA chip. The hybridization was performed at scalar temperatures (43 C, 45 C, 47 C, 49 C, 51C) for one hour. After hybridization the chips were scanned by GMS418 Array Scanner.

采用PCR引物直接标记法和掺入法对HPv标准品进行扩增和荧光标记，将标记好的标准品与芯片在梯度温度下(43℃，45℃，47℃，49℃，51℃)杂交1 小时，用GMs 418 Array Sc~er扫描仪对杂交后的芯片进行扫描检测分析，用 sca'Array软件对各信号点进行信号强度采集。

Against a backdrop of Wildean quotes and music by the Verve, Domenico and Stefano showed rococo Ruritanian military jackets with distressed jeans, shirts, and pants printed with equally rococo swagging, a biker jacket in astrakhan, and combinations of moiré and jacquard that hinted at an aristocratic opium dream. Floral-printed velvets, flocked brocade, and a tee printed with Jacques-Louis David's Leonidas at Thermopylae amplified the entirely aesthetic nature of the collection. Frankly, as the last show of Milan's men's calendar for Fall 2009, it was refreshing to see something that was reckless enough to go Out There.

援引了王尔德作品的背景，在the Verve的摇滚音乐的伴奏下，Domenico和Stefano带来了一系列充满浪漫皇室色彩的洛可可风格军装夹克，搭配同样装饰华丽的牛仔裤、衬衣和裤装；动辄整件铺满羊羔毛的机车夹克，以及大量云纹和提花织机组合图案的应用，十足的贵族气派；而那些印花天鹅绒，软毛金线织锦缎，以及印有法国古典浪漫主义画家Jacques-Louis David著名画作《雷奥尼达在温泉关》的T恤，则提升了整个系列的艺术气质。

To evaluate the specificity of the PCR, genomic DNA of Theileria annulata,Babesia bovis,Toxoplasma gondii,Leishmania donovani and standard strain of N. caninum were used as a template in the PCR. For determining the detection limit of amplification procedure, PCR was run on a dilution series of genomic DNA from N. caninum（1.562 5-200 ng/ml）. Brain tissue samples of 32 aborted fetuses were detected by PCR-based assay, and 23 blood samples from mothers were tested by ELISA. Results The amplified DNA fragment （350 bp）had a high identity of 98% with the Nc-5 gene sequence of N.

以环形泰勒虫、牛巴贝斯虫、刚地弓形虫、杜氏利什曼原虫以及犬新孢子虫标准株DNA为模板进行扩增以验证PCR的特异性，采用紫外分光光度计测定犬新孢子虫标准株DNA浓度和纯度，取高纯度的DNA样品用灭菌水稀释，分别取不同量的DNA进行PCR扩增，确定PCR方法的敏感性；利用该方法对32份奶牛流产的胎牛脑组织样品进行检测，同时，对其中23份流产的母牛血样进行ELISA血清学检测，以评价犬新孢子虫PCR方法的检测效果。

The genome DNA was extracted from the red blood cells or blood of the infected sheep and goats which include as isolates of ovine Babesia from these different geographic areas. The gene specific primers were devised according to the pubulished 18srRNA sequence at GenBank. The target DNA segment was amplified by polymerase chain reaction, the PCR product was ligated into PGEM-Teasy vector.

用分离于四个地区的羊的巴贝斯虫感染实验动物，自感染羊的红细胞或全血中提取基因组DNA，根据GenBank中已发表的巴贝斯虫18S rRNA序列设计两对羊巴贝斯虫特异性引物，通过PCR扩增目的基因片段，将扩增产物连接到PGEM-Teasy载体中，经酶切鉴定，PCR分析，进行序列测定，结果显示羊巴贝斯虫四个分离株的18S rRNA基因大小为1800bp左右。

RAPD (random amplified polymorphic DNA) analysis was employed in order to detect genetic relationship in 45 balsam pear Momordica charantia L.

利用RAPD技术对来自国内外的45份苦瓜栽培品种进行了亲缘关系分析。

RAPD analysis indicated that the profiles of mycelial protoplasts obtained from same single spore isolates amplified by 10 random primers were entirely identical, corroborating that the two nuclei of a basidiospore were homogeneous.

RAPD分析显示，源于同一单孢分离物的菌丝原生质体为10个随机引物所扩增的图谱彼此完全相同，印证了担孢子中的双核是同质的。

Boer goat ; billy goat ; fertility ; random amplified polymorphic DNA

波尔山羊；公羊；繁殖力；随机扩增多态DNA

DNA samples were harvested from 11 Boer billy goat and amplified by 10 selected arbitrary primers,and 113 markers including 31 polymorphic markers were observed.

用 10个随机引物对 11个波尔山羊精液样本DNA进行随机扩增，得到 113个标记，其中多态标记 31个。

The genetic structure of biotype B and biotype Q was analyzed using random amplifIed polymorphic DNA markers here.

本文利用RAPD分子标记对烟粉虱B型和Q型不同地理种群的遗传结构进行了分析。

His female bullfighter Lydia gradually Health love, but she was in a vegetative state after the Bulls Zhuangcheng away from her, just because Lydia's ex-boyfriend showed more affectionate than he is like love.

他对女斗牛士莉迪亚渐生爱意，却在她被公牛撞成植物人后离她而去，仅仅因为莉迪亚的前男友表现出好似比他更深情的爱。

I know of none which are not easy to answer, but what I am trying to do now is only by a series of hints to throw some light on the narrow path of legal doctrine, and upon two pitfalls which, as it seems to me, lie perilously near to it.

我不认为有什么难以回答的问题，但我现在要做的仅仅是通过一系列线索，来给法律理论的狭窄路径以及在我看来离它近得危险的两个陷阱提供一些启示。