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The flanking sequence of T-DNA insertion site was successfully amplified using TAIL-PCR in torenia, and the length of the amplified products ranged about from 200 bp to 2 000 bp and most of them were about 400 bp and 800 bp. Among them, 36% had homologous plant sequences.

采用TAIL-PCR成功扩增了转基因蓝猪耳T-DNA插入位点的侧翼序列，扩增片断长度为200~2000bp，大多数片段在400bp和800bp左右，其中36%的序列含有植物的同源序列。

The Laccase gene of Trametes versicolor's genomic DNA amplified by PCR using a set of primers that we designed in this study. The amplified fragment was cloned into the vector pUCM-T.

本研究以PCR扩增的方法，从变色栓菌的基因组DNA中扩增出了一预期大小的DNA片断，并将其克隆到了pUCM-T载体上。

Methods The genomic DNA extracted from the 2 trematodes was amplified with random amplified polymorphic DNA and then was carried on agarose gel electrophoresis.

应用随机扩增多态性DNA（Random amplified polymorphic DNA，RAPD）技术对斯氏肺吸虫和华支睾吸虫的基因组多态DNA进行扩增，扩增产物经琼脂糖凝胶电泳后分析结果。

Methods: Unrepeated MRSA strains from clinical specimens were divided into two groups: C-MRSA and H-MRSA, We used Chelex 100 to get DNA template and amplified mecA gene by PCR. The DNA templates of MecA were used to RAPD(random amplified polymorphic DNA), then we got the RAPD-DNA fingerprint-maps, randomly sampled 20 strains from each group as study objects.

收集临床MRSA标本，将其分为社区获得性MRSA与医院获得性MRSA两组，用Chelex 100裂解法制备DNA模本，PCR扩增mecA基因，选择mecA基因的DNA模本，用RAPD技术扩增出各菌株的RAPD-DNA指纹图谱。

If they mad amplified, I'm gonna be even more amplified.

如果他们疯放大，第二天我会更加充实。

The specific band EM433 was recovered., cloned and sequenced, and 2 primers of Sequence characterized amplified regions markers, 5 --CGAGACGTCCGAGTC GATATAGC-3 - and 5 --GTCGTCCTTCTAGTGGGTATCCTCC-3 -, were deviced and systhesised in terms of the result of sequence. 41 strains of V dahliae were amplified specifically, 9 nondefoliating strains and 2 defoliating strains could yield EM.

将特异片段EM_(433)进行回收、克隆、测序，在此基础上设计并合成两条SCAR标记引物5&-CGAGACGTCCGAGTCGATATAGC-3&和5&-GTCGTCCTTCTAGTGGGTATCCTCC-3&，对41个V.dahliae进行扩增，10个非落叶型菌系中有9个菌系可以扩增出特异片段，31个落叶型菌系中29个菌系无此特异片段，鉴定准确率可达90％以上。

Amplified fragment length polymorphism was performed on 41 strains of V dahiiae of cotton in search of DNA probes specific for distinguishing between nondefoliating strains and defoliating strains. Specific bands of 433 base pairs and 1 lObp amplified only from nondefoliating strains were obtained using two pairs , E64 M53 and E49 M65 ,which named EM433 and EM110, respectively.

选用25对EcoRI和MseI引物组合，对供试的41个V.dahliae进行AFLP扩增，筛选到两对引物E64、M53和E49、M65，能分别扩增出433bp和110bp两条仅为V.dahliae非落叶型菌系独有的特异片段，可将落叶型与非落叶型菌系分开，这两条特异片段被命名为EM_(433)和EM_(110)。

It was found that the sequences amplified from the CHD-W gene using primers P2、P8 were more conservative, the sequences amplified from the CHD-Z gene had many variation, and that the length of Z chromosome had polymorphyism during the sex identification of Chinese Egret.

发现引物P2、P8扩增的CHD-W基因的片段较为保守，而扩增的CHD-Z基因片段变异较大，而且在对黄嘴白鹭的性别鉴定过程中发现Z染色体长度多态现象。

The proviral DNA was amplified by PCR using primers gag1 or tax1. The amplified products were detected with agarose gel electrophoresis.

结果］ 2株都扩增出了HTLV-I前病毒DNA的gag1和tax1片断，证明了嗜人T细胞白血病病毒I型的分离成功。

After purification and quantification,1 ugof total RNAs were reverse transcribed and amplified by PCR with5'upstream 10 base random primers and one-base or two-base anchored 3'primer respectively,and the amplified products were displayed on asequencing gel side by side.

本文以父本黄早4，母本U8112和杂种一代掖单4号的玉米幼苗为材料，提取总RNA，用5'端随机引物和3'端单锚定或双锚定引物，进行了mRNA的差异展示研究。

Cable dampener cannot be rolled up but must be hung over the cable.

缆帆不能被卷起，必须保证处于展开状态

"This skylark is a present from my master," he says.

"这只云雀是我主人献上的礼物。"他说。