查询词典 amplification factor

Methods and Results— We synthesized the 3 components of a gene vector binding complex:(1) A poly allylamine bisphosphonate with latent thiol groups,(2) a poly ethyleneimine with pyridyldithio groups for amplification of attachment sites , and (3) a bifunctional (amine- and thiol-reactive) cross-linker with a labile ester bond.

方法和结果—我们综合了基因复合物载体的三种成分：(1)巯基聚丙烯胺二碳磷酸盐化合物，(2)能扩增结合位点聚乙烯亚胺-pyridyldithio复合物，(3)具有不稳定酯键的双功能交联剂。

In the T1 generation, ten from 30 plants showed kanamycin-resistance. And, PCR amplification confirmed that 8 plants of them had specific fragment of chitinase, but no specific fragment ofβ-1, 3-glucanase. In this study an amaranth agglutinin gene was also cloned from Amaranthus retroflexus for obtaining aphids resistant transgenic cotton.

对卡那霉素有抗性植株的T1代进行盆栽检测，30株中表现卡那霉素抗性的植株有10株，其中8株在几丁质酶基因的检测中表现出阳性，但相同植株在β-1， 3-葡聚糖酶基因的检测时无目的条带出现。

Transgenic plants expressing GNA showed significant insecticidal activity towards \\omoptera insects such as aphids and brown planthopper in bioassay and feeding tests.In this paper a lectin gene was firstly isolated from leaves of Zephyranthes grandiflora, belonging to Amaryllidaceae, by the use of rapid amplification of cDNA ends technique. Characterization, functional prediction and construction of plant and prokaryotic expression vector of this novel gene were studied.

本实验采用cDNA末端快速扩增(Rapid amplification of eDNA ends，RACE)技术，首次从中国特有的野生植物韭莲(又名风雨花，Zephyranthes grandiflora)的叶片中分离克隆出了一个新的凝集素基因，对基因进行了序列分析和功能预测，并成功地构建了韭莲凝集素基因的植物表达载体和原核表达载体。

RT-PCR amplification of ginseng β-amyrin synthase gene was successfully performed based on the total RNAs extracted from ginseng hairy roots induced by Agrobacterium rhizogenes A4 using a modified guanidine isothiocyanate-method.

以发根农杆菌A4菌株诱导的人参发根为材料，用改良的异硫氰酸胍法提取总RNA，得到了纯度好的完整的总RNA。

80 Individuals of ATA-A F2 plants were used for RAPD molecular mapping of the monoecious gene by bulk segregant analysis. 840 random primers were chosen for polymerase chain reaction amplification between monoecious pool and andromonoecious pool, the results showed that: primer S128 was tightly linked to the monoecious character, and primer S493 was tightly linked to the andromonoecious character.

以甜瓜单性花系ATA-A系与两性花品种YJX-1的杂交F2代的80个单株为材料，利用群分法进行单性花基因有关RAPD分子标记研究，筛选分析了840个10bp随机引物的PCR扩增谱带，结果表明，随机引物S128在1180bp，S493在930bp处产生与性别相关的特异性条带，S128的特异条带与单性花性状相关，S493的特异条带与两性花性状相关。

MATERIALS AND METHODS: Using telomeric repeat amplification protocol assay and ABC immunohistochemical technique, the level of telomerase activation and PCNA expression were evaluated in 29 skin lesions of arseniasis patients and 10 cases of normal skin.

材料与方法：采用端粒重复序列扩增法和免疫组化ABC法检测29例砷中毒患者病损皮肤及10例正常皮肤组织中的端粒酶活性和PCNA表达情况。

Five methods namely improved CTAB method, CTAB-silica extraction method, SDS method, low pH extraction method with high salt and Zhou shiliang method were adopted to extract genome DNA from the leaves of Citrus aurantium. And three methods including ultraviolet spectrophotometer, agarose gel electrophoresis and SSR-PCR amplification were used to detect extracted sample DNA.

以枳壳叶片为试验材料，分别采用改良CTAB法、CTAB-硅珠法、SDS法、高盐低pH值法和周世良法5种方法提取枳壳基因组DNA，并通过紫外分光光度计、琼脂糖凝胶电泳及SSR-PCR扩增3种方法对所提取的DNA样品进行检测。

Furthermore, cell culture and amplification are comparatively easy so we can get a great quantity of cells applied by autografting and allografting.

目的：探讨由脂肪组织分离获得脂肪基质细胞的方法及其在体外经诱导培养后向成骨细胞分化的潜能。

Some taxon-selective primers that were intended to be specific to fungi and basidiomycetes respectively have been designed and tested to characterize fungal symbionts by amplification of specific sequences in the nuclear ribosomal unit.

大量DNA分析新技术在丛枝菌根真菌的分子遗传、分类鉴定、种间及种内亲缘关系、菌株持久性等方面得到应用，与传统菌根研究方法相比，表现出巨大的优越性。

Transgenic plants were obtained by basta screening and PCR amplification.

转化T1代植株经除草剂Basta筛选和PCR扩增获得转化植株。

The quality of both red and white wine depends very much on the wine maker talent and on the quality of the grapes.

他的红酒和白酒的品质，根据酿造者的能力和葡萄的质量，各有差异。

No, I don't want to get drunk before my next match.

" "不，我不想让我的下一场比赛前喝醉了。