查询词典 amplification factor

Darkroom equipment: We have to wash film and enlarge photos with the equipment listed together, must be equipped with are: Developing tank, roll film plate, amplification machine, zoom lenses, thermometers, measuring cups, storage containers, plates, clips, safety lamp.

暗房设备：我们洗电影和扩大同列在一起的照片的设备，必须有：显影罐，胶卷板，放大机配备变焦镜头，温度计，量杯，容器，盘子，夹子，安全灯。

They include the variation of the degradement velocity of different sample, the inhibition product of PCR, the amount of DNA extraction, quality of DNA and the heteroplasmy of some tissue. Correct sequencing, comparison of the result of forward and reverse primers sequencing should be done to rule out wrong signals, and prevent contamination. In addition to gene sequencing, low grade heteroplasmy could be dealt by Denaturing high performance liquid chromatography. Multiplex Ligation-dependent Probe Amplification. The analysis of mitochondrial HV Ⅰ, HV Ⅱ sequencing is able to identify the maternal sanguinity, then it could assist in the human identification. It also complements the genomic STR system in distribution of population, the origin of human, and analysis for fossil.

分析粒线体DNA时必须考虑各类检体腐败DNA降解速度之不同、PCR的抑制物质、DNA的萃取量及品质、特定组织的异质现象、萤光序列分析图及正、反向引子定序结果比对、排除杂讯干扰、防治污染等，除以基因定序外，低程度的异质现象也可用Denaturing high performance liquid chromatography、Multiplex Ligation-dependent Probe Amplification等方法分析，粒线体HV Ⅰ、HV Ⅱ碱基序列的分析可鉴别一个人的母系血缘关系、辅助人类身分鉴定，对於族群分布、人类起源探讨、远古检体的分析可作为辅助STR系统的利器。

The isothermal amplification step depends not only on the replication of DNA polymerase, but also on the scission of nicking enzyme, which can generate a great number of aptamer.

我们用该方法对细胞色素P450酶系中的异喹胍羟化酶的单碱基多态性(CYP2D6*10)进行了检测，使突变型和野生型基因DNA得到了很好的区分。2。

Mitochondrial DNA extraction, long-PCR amplification and primer-walking sequencing methods were carried out for determining the complete mt DNA sequence of Scolopendra multilan.(1) The complete mitochondrial genome of Scolopendra multilan is 13,734 bp in size.

采用长片断扩增和PCR引物步移测序等方法，补齐少棘蜈蚣线粒体基因组全序列，主要结果如下：（1）序列全长为13，734 bp，其基因组成和顺序、基因的方向即编码链的选择、碱基G的低含量和对碱基A偏好以及GC和AT偏斜与已知的多足动物线粒体基因组相同。

With the method of Bulked Segregant Analyis, 300 randomly selected 10bp primers were used in PCR amplification of the DNA pools from gynoecious and monoecious cucumber, respectively, and polymorphism were found in five primers in three replications.

共选用 30 0个 1 0碱基的随机引物按BSA法对黄瓜性别表型分离群体进行PCR扩增，筛选出 5个在全雌和弱雌株基因池中表现多态性的引物。

In this study, homologous amplification and RACE technique were used to clone the encoding gene (SpTPS1) of trehalose-6-phosphate synthase from Selaginella pulvinata, which is an endemic exrophilous plant in China. The full-length cDNA is 3223 bp long, containing an open reading frame of 2790 bp.

本研究以我国特有旱生植物垫状巻柏为材料，采用同源扩增与RACE技术相结合的方法克隆了海藻糖-6-磷酸合成酶基因SpTPS1，cDNA全长3223 bp，包括一个2790 bp的开放阅读框。

As high sensitiveness of ABI3730XL DNA analyzer, common PCR amplification reaction system was not suitable for the detection system.

由于ABI3730XL DNA分析仪的灵敏性很高，普通的PCR扩增反应条件不适应其检测系统。

Inaddition,we extract total RNA from cultural U251 cell of humanglioma,make coding gene extron amplification of TfR through ReverseTranscription-Polymerase Chain Reaction,construct expressionplasmid of pCDNA3.1-TfR after digestion of enzyme andconjunction,do transfection to U251 cell by liposome afteridentification,cause excess expression,detect the efficiency of transientexpression of TfR protein by pEGFPN1-TfR, sieve positive cell ofpCDNA3.1-TfR through G418, choose positive cell clone, detect theexpression of TfR in anteroposterior period through Western blot,FCM,cell immunochemistry and mRNA through RT-PCR, identify the effect of transfection.

另外，培养人脑胶质瘤U251细胞，进行细胞总RNA抽提，运用RT-PCR方法进行TfR基因的完整编码区外显子的扩增，经过酶切、连接后构建pCDNA3.1-TfR的表达质粒，鉴定正确后按脂质体转染方法转染人胶质瘤U251细胞，使其过量表达，通过pEGFPN1-TfR检测其瞬时转染的效率；用G418对稳定表达pCDNA3.1-TfR的细胞进行筛选，挑选阳性细胞克隆，运用Western blot、流式细胞检测技术和细胞免疫化学以及mRNA半定量的方法检测TfR在转染前后的表达水平，鉴定转染的效果。

The effect of pulse width on soliton amplification has been studied.

为了获得更短的光脉冲，脉冲放大技术已成为研究的热门课题。

Stagger studded for tremendous soundproofing and meticulously designed and constructed to allow perfect amplification, the sound quality is incredible without disrupting adjoining spaces.

隔音墙带来惊人的隔音功能，加上精工设计打造，造成完美的放音效果，不可思议的声音品质，却不致干扰到邻近空间。

There are different types of personal loans available.

有许多不同类型的个人贷款优惠。

The above mentioned teaching model of spiral rising can be used to avoid rigidness in teaching.

上述螺旋式上升的教学模式，可以避免教学形成模式後的僵化现象。