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We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

However, if your first cash-in amount is less than the bonus amount and there are further funds in your casino account, the cash-in amount will be removed, and the remainder (to make up the full bonus amount) will be subtracted from your casino account.

不过，如果你的第一个现金数额比奖金数额减少，这个时候，在赌场的帐户更多的资金，现金数额将被删除，其余的将于减去您的赌场帐户。

Less amount of essence if used in floral water, about 5 percent (including 5%), a large amount of alcohol is used, but the density is low and a small amount of cassia oil needs to be added, herba agastachis oil and other raw materials need to be added, a large amount (over 5%) of essence is needed for perfume, less alcohol is used, but the density is high.

花露水香精用量少，在5％（含5％）以下，酒精用量多，但浓度低，且要加入少量桂皮油、藿香油等原料；香水香精用量大，在5％以上，酒精用量少，但浓度高。

Recent research shows that 5 gram of Young Barley Grass Powder contains equivalent amount of dietary fibre to 170 gram of apple or 270 gram of vegetable; equivalent amount of calcium to 400 ml of bovine milk; equivalent amount of iron to 35 gram of spinach and equivalent amount of carotene and vitamins to 170 gram of tomato.

最新科学研究发现，每5克的大麦嫩芽粉中，膳食纤维相当于170克苹果或270克青菜，含钙相当于400ml牛奶，含铁相当于35克菠菜，胡萝卜素和维生素相当于170克西红柿。

Obviously, the optimization management in middle-sized or small cities of China is very necessary. The basic characteristics of municipal solid waste generated in middle-sized or small cities of China are put forward in the paper. The total amount of municipal solid waste generated in middle-sized or small cities is significantly less than that in large cities. The amount of garbage is about 1 kg per capita and year. In the garbage, inorganic material is more than organic material, the combustible less than incombustible and the amount of uncompostable garbage more than the amount of garbage that can be disposed for composting. The value of heat of garbage is about 800 kal/kg.

通过总结，我国中小城市生活垃圾的基本特点为：各城市的生活垃圾产生量相对较小，人均生活垃圾产生量大约在1kg/d左右；垃圾组分中以煤渣、灰土为主的无机成分含量过高，可回收物质成分含量很低，不可燃成分多于可燃成分，不可堆腐成分多于可堆腐成分，垃圾热值大约在800kal/kg左右；并且在我国，南方城市与北方城市，经济发达城市与欠发达城市之间的生活垃圾产生量与组成也存在着明显的地区差异。

Through the photographic technology of fisheye lens and achieved the space image of cotton canopy structure distributing at different condition, and setupped cotton canopy structure base after image analysis. The experiment results indicated that the adaptability of different varieties to plant density is different, reasonable plant density is benefit to the raise of varieties product, and Xinluzao10 is better at high density（225000plant/ hm2） condition and Xinluzao12 is better at mid-high density（150000 plant / hm2） condition. For different nitrogen, Xinluzao10 is better at right nitrogen application amount（600 kg/ hm2） condition and Xinluzao12 is better at mid-high amount（900kg/ hm2）condition. For different irrigation, Xinluzao6 and Xinluzao12 is better at limited drip irrigation amount（1500 m3/ hm2） condition and Xinluzao8 Xinluzao13 is better at right drip irrigation amount（3000 m3/hm2） condition.

利用鱼眼镜头照相技术获取了不同处理棉花冠层分布空间图象，对图像进行处理分析，建立了棉花冠层图像库及分析诊断指标，研究结果表明，不同品种对种植密度适应性不同，合理的配置种植密度有利于品种的产量潜力的挖掘，高密（225000plant/ hm2）条件下选择新陆早10号、中高密（150000 plant / hm2）条件下选择新陆早12号栽培种植较为适宜；对于不同氮肥用量，适量氮肥用量（600 kg/ hm2）条件下选择新陆早10号、中高氮肥用量（900kg/ hm2）条件下选择新陆早12号较为适宜；对于不同灌水量，限量滴灌（1500 m3/ hm2）条件下选择新陆早6号、12号种植较适宜，适量滴灌（3000m3/hm2）条件下选择新陆早8号、13号种植较适宜。

The soil erosion process on the slope was clearly divided into sheet erosion stage, rill erosion developing stage and rill erosion stable stage. Before lOminutes of rainfalls, the percentage of sheet erosion amount to total erosion amount was more than 90%, and it indicated that sheet erosion happened on the slope. This percentage sharply decreased before SOminutes of rainfall time, on the other hand, the percentage of rill erosion amount to total erosion amount increased in curve form with rainfall time.

在降雨前10min左右，面蚀贡献率约占坡面侵蚀总量的90%以上，为面蚀阶段；随着降雨时间的延续，面蚀贡献率先急剧递减最后在第30min左右基本达到稳定，细沟侵蚀贡献率则出现相反趋势，此为细沟发育阶段；降雨后期，面蚀和细沟侵蚀贡献率的变化趋势逐渐变缓且基本达到稳定。

CD8~+ cell is the main T lymphocyte subset in spleen, and B lymphocyte mainly is IgG~+ cell, moreover the amount of these B lymphocytes could exceed CD3~+ T lymphocyte subset after 7 days. CD8~+ cell is the main T lymphocyte subset in tonsil of appendix, and B lymphocyte is IgM~+ cell, and the amount could exceed CD3~+ T lymphocytes after 35 days. After 21 days, B lymphocytes in esophago tonsil are the main IgA~+ cells and the amount exceeds CD3~+ lymphocytes. The amount of CD4~+ lymphocytes is more than CD8+ lymphocytes.4. CD3~+、CD4~+ and CD8~+ T lymphocytes in spleen mainly distribute in periarterial lymphoid sheath. However IgM~+、IgG~+ and IgA~+ cells mainly distribute in ellipsoid periarterial lymphoid sheath and germinal center. T lymphocytes in appendix tonsil mainly distribute in middle and inferior part of mucous and the B lymphocytes mainly in middle and mucous between 4~7 days. Whereafter T, B lymphocytes equably distribute in mucous. CD4~+ cells arrange tightly and mainly occupy the central part in aggregates of T lymphocytes in esophago tonsil and CD8~+ lymphocytes mainly distribute in periphery. Meanwhile B lymphocytes encircle the periphery of aggregates of T lymphocytes. The aggregates of B lymphocytes is mainly the germinal center with lots of IgM~+、IgG~+ and IgA~+ cells. Meanwihle T lymphocytes encircle the periphery of aggregates of B lymphocytes.5. There is an intimate relationship between the development of tissue structure of peripheral immune organs and lymphcytopoiesis. The maturation of tissue structure is stimulated by the immigration of lymphocytes and the mature tissue structure provides place where lymphocytes grow mature and functionate.

脾脏在21日龄时达到稳定，食管扁桃体和盲肠扁桃体均在35日龄时达到稳定；脾脏中T淋巴细胞亚群以CD8~+细胞为主，B淋巴细胞则以IgG~+细胞为主，并在7日龄后数量超过CD3~+T淋巴细胞；盲肠扁桃体中T淋巴细胞亚群以CD8~+细胞为主，B淋巴细胞以IgM~+细胞为主，并在35日龄后数量超过CD3~+T淋巴细胞；21日龄后，食管扁桃体中B淋巴细胞以IgA~+细胞为主，数量超过CD3~+细胞，CD4~+细胞的数量多于CD8~+细胞。4、在T、B淋巴细胞组织定位方面，脾脏中CD3~+、CD4~+和CD8~+T淋巴细胞主要分布在动脉周围淋巴鞘中，而IgM~+、IgG~+和IgA~+细胞主要分布在椭球周围淋巴鞘和生发中心中；4～7日龄时，盲肠扁桃体中T淋巴细胞主要分布在粘膜固有层的中下部区域，而B淋巴细胞则主要分布在中上部区域，随后各日龄T、B淋巴细胞均匀地分布在粘膜固有层中；在食管扁桃体的T淋巴细胞聚集物中，CD4~+细胞紧密排列，主要占据中央部位，CD8~+细胞主要散布在外周，同时B淋巴细胞又环绕在整个T淋巴细胞聚集物的外周；B淋巴细胞聚集物主要为生发中心，其中存在大量IgM~+、IgG~+和IgA~+细胞，同时T淋巴细胞又环绕在整个B淋巴细胞聚集物的外周。5、外周免疫器官的组织结构发育和淋巴细胞发生之间存在密切的关系，淋巴细胞迁入淋巴器官刺激组织结构的发育成熟，同时成熟的组织结构又为淋巴细胞发育成熟并行使功能活动提供场所。

The amount of different mineral elements in different parts of male and famale B. thoracica had big difference, except Zn; the amount of other mineral elements in female breast muscle were all higher than that in male breast muscle, and the amount of Ca, Fe in leg muscle, Ca, Cu, Mn in skeleton and Cu, Mn in feathers of male were all higher than that of female, but the amount of Ca, Fe, Zn in feathers of female were higher than that of male.

雌雄个体不同部位、不同矿质元素含量存在较大差异，除Zn外，雌性胸肌中其他元素的含量高于雄性，雄性腿肌中Ca、Fe含量、骨骼中Ca、Cu、Mn含量、羽毛中Cu、Mn含量均高于雌性，雌性羽毛中Ca、Fe、Zn含量高于雄性。

On the base of probabilistic and statistics results of joints and cracks, the Author modifies Block Theory. The sliding probability of Key Block is I A RSTR A CT F2 ? 5.It is put up forward that GM(l,1) Model to predict Amount of rainfall, and Markov Chain to modifY GM( 1,1 )predicting result, and then a theoretical predicting Model of amount of rainfall is firstly built. This model is applied in a situ example. It is proved that the model is very useful to predict amount of rainfall ,when ordinal array of Amount of rainfall changes in unexact index rule.

首次将节理裂隙视为有限长，借助有关岩体节理裂隙概率统计研究成果，对块体理论进行修正，确定了关键块体变为滑动块体概率计算公式为 5、本文提出采用GM（1，1）模型预测降雨量，而采用Markov链进行修正的方法，首次用该方法建立了大气降雨量预测的理论模型，并结合实例进行具体应用，解决了当降雨量的时间序列呈不严格的指数规律变化时，大气降雨量的预测问题。

The most precious possession that ever comes to a man in this world is a womans heart.

在这个世界上，男人最可贵的财产就是一个女人的心。

Some 4 years above company and divisional management experience, 6 year foreign enterprises work experience.

有4年以上的公司及部门管理经验，6年外企工作经验。