查询词典 adherence

Get the neonate Sprague-Dawley rats medulla spinalis, through the conventional primary cell culture procedure, we use the method of difference adherence time and get the motor neurons of anterior spinal cord. When the neuron is in maturity, we make the mechanical injury model in vitro. All the models were divided into four groups: group A is control group; group B is 100μM ATP group; group C is 100μM ATP+20μg/ml suramin group and group D is 100μM ATP+10μM ouabain+10μg/ml Thapsigargin group. Culture the four groups neurons, after one day, we count the motor neuron, observe the survival and activity of neurons through MTT shade selection experiment, use flow cytometry to analyze the percentage of apoptosis of motor neurons of anterior spinal cord and detect the expression of protein p-GSK-3β(ser9) through Western-Blot technology.

方法取新生大鼠脊髓，通过常规的原代细胞培养程序，采用差速贴壁法分离出大鼠脊髓前角运动神经元，培养成熟后制作神经元机械损伤体外模型，分为A组：对照组、B组：100μMATP组、C组：100gMATP+20μg/ml Suramin组和D组：100μM ATP+IOμM Ouabain+10μg/ml Thapsigargin组，对各组机械损伤的运动神经元进行培养，1天后分别进行运动神经元计数、MTT比色实验观察运动神经元的存活及活性、流式细胞仪分析机械损伤的脊髓前角运动神经元凋亡百分率和Werstern Blot技术检测p-GSK-3β(ser9)的表达。

METHODS: Triceps brachii was sterilely collected from 3 rats, and muscle-derived stem cells were isolated in vitro, and purified by adherence method.

取3只大鼠，无菌条件下切取肱三头肌，体外分离培养肌源性干细胞，贴壁法纯化扩增。

METHODS: Bone marrow was sterilely separated from human. After heparinization, human BMSCs were harvested using density gradient centrifugation and adherence method. At the fifth passage, BMSCs at 1×108/L were incubated in the 6-well plate and divided into 2 groups. BMSCs in the edaravone group were 50% confluent and incubated in L-DMEM containing basic fibroblast growth factor and fetal bovine serum for 24 hours. After washing in PBS, these BMSCs were incubated in serum-free L-DMEM containing 20 mg/L edaravone for 24 hours. BMSCs in the blank control group were incubated in L-DMEM, supplemented with 10% fetal bovine serum.

无菌抽取的骨髓经肝素化后，采用密度梯度离心法及贴壁筛选法分离获得人骨髓间充质干细胞，传至第5代按1× 108 L-1接种于6孔板内，设立2组，依达拉奉组细胞达50%融合时用含碱性成纤维生长因子、胎牛血清的L-DMEM预诱导24 h，PBS洗涤后再用20 mg/L依达拉奉无血清L-DMEM诱导24 h；空白对照组始终用含体积分数为10%胎牛血清的L-DMEM培养，不加任何预诱导剂和诱导剂。

METHODS: Rat double lower limbs were isolated sterilely. Bone marrow was obtained by rinsing using low-glucose DMEM. BMSCs were isolated and purified by the whole bone marrow adherence method and density gradient centrifugation.

无菌条件下分离大鼠双下肢，低糖DMEM培养液冲出骨髓，分别运用全骨髓贴壁法和密度梯度离心法体外分离骨髓间充质干细胞，利用差速贴壁原理对细胞进行纯化扩增。

METHODS: Bilateral sciatic nerve was sterilely dissociated from rats. Schwann cells were prepared and purified by low-concentration enzyme fast digestion and differential adherence.

无菌剥离大鼠双侧坐骨神经，应用低浓度酶快速消化法、差速贴壁法分离培养纯化许旺细胞。

METHODS: Bilateral testis of Kunming male mice aged 5-7 days were sterilely collected. Spermatogonial stem cells were isolated and purified using adherence and noncontinuity Percoll density gradient centrifugation.

无菌收集5~7 d龄昆明雄性小鼠双侧睾丸，采用差速贴壁联合非连续性Percoll密度梯度离心法分离纯化精原干细胞。

laparoscopic procedures were completed in 24 cases. only one case of the transperitoneal group was transfered to open surgery for severe adherence.

1例早期经腹腔入路手术的病例在术中发现肿瘤粘连紧密改为开放性手术，其余24例在腹腔镜下顺利切除肿瘤。

A prospective study of patient adherence to topical treatments: 95% of patients underdose.

对依从局部治疗的患者进行一项前瞻性研究：95％的患者用药剂量不足。

ISO pretreatment had no effecton arteriolar and postcapillary venular blood flow velocityin LPS rats or leukocyte adherence in LPS or control rats.

预防性使用 ISO 不会影响 LPS 组的小动脉和毛细血管后小静脉的运动速率，也并不影响 LPS 组和对照组白细胞的附着性。

ISO pretreatment had no effecton arteriolar and postcapillary venular blood flow velocityin LPS rats or leukocyte adherence in LPS or control rats.

预防性使用 ISO 不会影响 LPS 组的小动脉和毛细血管后小静脉的运动速率，也并不影响 LPS 组和对照组白细胞的附著性。

I was just taking her hand, to help her out of a car, and I knew it.

我只是握着她的手，扶她下车，但我知道这一切。

China and South Korea have excoriated Japan over its approval of new school books which they say whitewash the atrocities committed during Japanese occupation, and anti-Japanese protests have been held in Chinese cities. The lingering bitterness over Japan's past imperialism still threatens to mar relations between the big East Asian powers

中国和韩国强烈谴责日本通过新的中学历史教科书，他们认为这一教科书掩盖了日本在其占领期间所犯下的残酷暴行，反日抗议活动在中国数座城市发生，历史上日本帝国主义制造的痛楚无法被忘却，这有可能破坏东亚强国间的关系。