外植体

基本解释 (translations)

explant  ·  explanted  ·  explanting  ·  explants

与外植体相关的网络例句 [注：此内容来源于网络，仅供参考]

The plant expression vectors were constructed and two genes were transferred into A. thaliana and N. tabacum, antikanamycin plants were obtained.4. The trials of adventive bud regeneration directly and callus induction was conducted to select out the optimal explants and formula. The results revealed that double scale can produce 13~16 adventive buds per centimeter in MS medium added 10.0 mg/L 6-BA, 1.0 mg/ L NAA, 40 g/L sugar and 7.5 g/L agar. Scale, leaves, scape and different tissue of flower can be induced to produce callus in appropriate medium, but the filament with anther was the optimized explant.

构建了两个基因的pBI121和pCAMBIA1300植物表达载体，并通过农杆菌将上述基因转入烟草和拟南芥中，得到了卡那抗性的再生植株。4、以中国水仙不同部位外植体进行了不定芽直接分化和愈伤组织诱导分化试验，结果表明，双鳞片外植体在MS0+6-BA 10.0 mg/L+NAA 1.0 mg/L+蔗糖40 g/L+琼脂7.5 g/L培养基上可以大量地直接再生，再生芽数量平均可达13~16个/cm双鳞片，比传统的鳞茎盘之间分化提高30~50%；鳞片、叶片、花葶及花部不同组织在适宜的配方中均可不同程度地诱导出愈伤组织，但以带花药的花丝诱导出的愈伤组织最好。

Result］ The new buds and petals of I.germanica was proper to be used as explant of tissue culture, with lower browning rate compared with other positions. The well situated tissue block of explant (3 mm×3 mm×3 mm) might reduce browning rate. Adding 0.1%Vc or 0.3% active carbon in medium had obvious effect on preventing tissue browning. The exogenous hormone 2 mg/L 6-BA was easy to lead to brownness turning, and 2 mg/L Kt and 2 mg/L NAA brought less effect to tissue browning. When using disinfectants of alcohol, its dipping time of 5～10 s for explant was the best, with the minimum browning rate. The culture under full illumination condition was liable to induce brownness turning, compared with other cultured conditions, the brownness turning could be restrained effectively by using imitating artificial climate condition for culture.

结果］ 鸢尾的新生芽和花瓣较适宜作组织培养的外植体，与其他部位相比褐变率较低；外植体组织块适中（3 mm×3 mm×3 mm）可降低褐变率；在培养基中加入0.1%Vc或0.3%活性炭对防止组织褐化效果明显；外源激素2 mg/L 6-BA易引发褐变，2 mg/L Kt和2 mg/L NAA对组织褐变影响不大；使用乙醇消毒剂时，对外植体浸泡时间以5～10 s为佳，褐变率最低；全光照条件培养容易诱发褐变，采用仿人工气候条件培养较其他条件培养可有效抑制褐变的发生。

Study on the rapid Propagation Technique of Lycoris. Herb and get follow results: In the period found of axenic clone 0.1%HgCl2 is the best disinfector to deal with the Lycoris" bulb ,as to neat part, such as root, leave, and bulbil, is fit to use 0.1%HgCl2 antisepsis 7min. And found the best effect is the bulb scale with base. Root, leaf and bulb scale without base all were not inducement adventitious buds. Different position of bulb had different culture effect. 3-15 of middle part of bulb can be induced most adventitious buds but inner and outer of least it. Incised the bulb with three types (pieces of eight, pieces of six, piece of four), and found the type of pieces of six is best to Lycoris mass production. L.sprengeri is fit MS+BA5mg/l+NAA0.1mg/l and L.squamigera is fit MS+BA5mg/l+NAA0.5mg/l, but the medium fit to culture L.longituba haven"t be found.In the period of Subculture-Found of mass production, the most multiplication of adventitious buds in MS+BA5mg/l+NAA0.5mg/l during subculture of L.sprengeri, L.squamigera.

石蒜属植物快速繁殖技术研究中，在无菌无性系建立阶段：鳞茎以0.1％HgCl_2消毒10-12分钟效果最好，而叶、根尖、鳞芽等较干净部位选用0.1％HgCl_2消毒7分钟；用三种石蒜属植物的叶片、根尖、鳞芽及带基盘与不带基盘鳞片进行培养，以带基盘鳞片诱导分化效果最理想，鳞芽易培养出芽，但数量有限，而叶片、根尖与不带基盘鳞片均未诱导分化；带基盘鳞片为石蒜属植物快速繁殖最佳外植体，以鳞茎中部3-15层芽诱导率高，较外层稍次之，内部鳞片诱导率最低；选用八等分法、六等分法、四等分法切割鳞茎，六等分法综合效果最好；每外植体带三鳞片培养最为适宜；三种石蒜属植物各自适合的培养基成分不同，换锦花在MS+BA5mg／l+NAA0.1mg／l中培养效果最好，夏水仙为MS+BA5mg／l+NAA0.5mg／l，而白花长筒石蒜在各培养基组合中培养效果均不理想，其适合的培养条件有待于进一步研究。

0Mg/L 6-benzylaminopurine was the optimal concentration of shoots quantity inducing which frequency of inducing shoots and number of shoots per explant were 100% and 11.1 respectively. 0.5mg/L 6-benzylaminopurine was the optimal concentration of shoots length inducing which average length of shoots was 12.0mm. Explant size had significantly effects on direct shoots quantity and length induction. When complete cotyledon used as explant, frequency of inducing shoots, number of shoots per explant and average length of shoots could approach to 100%, 6.7 and 17.1mm respectively. 2.0mg/L silver nitrate could significantly increase shoot quantity induction, both frequency of inducing shoots and number of shoots per explant could be as high as 97.2% and 4.2 respectively. But silver nitrate also could restrain shoots elongation.

结果表明：不同黄瓜品种直接不定芽数量诱导存在显著差异，但长度诱导无显著差异；6-苄氨基嘌呤对直接不定芽数量和长度的诱导作用显著，其中4.0mg/L为数量诱导适宜浓度，其出芽率和每外植体出芽数达到最高，分别为100.0%和11.1，0.5mg/L为长度诱导适宜浓度，其平均芽长12.0mm；外植体的大小对直接不定芽数量和长度均具显著影响，随着子叶的增大诱导直接不定芽的数量和长度增加，其中单片完整子叶为最适宜大小，其出芽率、每外植体出芽数和平均芽长分别达到100%、6.7和17.1mm；硝酸银也对直接不定芽数量诱导影响显著，其中2.0mg/L为适宜浓度，出芽率和每外植体出芽数分别达到97.2%和4.2，但对芽长有抑制作用。

It turned out reproduction rete of chrysanthemum was raised,the regular,non-plant diseases and insect pests could be offered to the market. Shoot tip were used as explants to study the effects of different sterilization methods, illumination intensity and concentrations of 6-BA and NAA on shoot propagation. Rooting with perlite, vermiculite and river sand instead of agar was studied. In the process of preliminary multiplication, the effect of using 0.1% HgCl_2 in sterilization of explants was better than using saturated bleaching solution. The highest multiplication rate was found under whole illumination. MS media with 6-BA from 0.2 mg·L~(-1) to 5 mg·L~(-1) and NAA from 0.05 mg·L~(-1) to 2 mg·L~(-1) were tested and as a result, the highest differentiation rate and the multiplication rate was reached with 0.2 mg·L~(-1) 6-BA, 0.05 mg·L~(-1) NAA.

本实验以菊花的茎尖为外植体，研究了不同灭菌方法，光照强度，全光照、半光照和弱光处理以及不同6-BA和NAA浓度及配比对于试管苗增殖的影响，并研究了珍珠岩、蛭石和河砂等琼脂替代物对于菊花生根的影响，结果表明：在初代培养物建立的过程中采用0.1％升汞进行表面灭菌的效果好于饱和漂白粉的灭菌效果；在全光照条件下外植体的增殖倍数最高；在试管苗增殖培养的过程中，以MS为基本培养基，并在培养基中添加0.2～5mg·L~(-1)6-BA和0.05～2mg·L~(-1)NAA，其中0.2mg·L~(-1)6-BA和0.05mg·L~(-1)NAA最适于外植体的分化和增殖，其分化率为100％，增殖倍数为12。

The small bulbs induced were full and the shapes of them were regular. The explants incubating in other seasons were not suitable. By comparison different incision methods, we found it' was better taking stem plate carrying bulb leaves as explants than any other treatments.

通过对不同季节的水仙鳞茎外植体进行组培观察，发现8月份至次年5月份接种的水仙外植体，小鳞茎的诱导率几乎达到100%；并且，小鳞茎比较饱满，形态比较规则，其它时间接种的水仙外植体的组培效果均不理想。

In order to breed new germplasm for Narcissus tazetta,the bulb of Narcissus tazetta is used as explants.The adventitious buds is induced in MS media with hormone combination.γ-ray irradiation treatment of Narcissus tazetta combined with tissue culture is studied.Three materials,including the bulb basal plate tissues inoculated for 7 days,tissues transferred in fresh differentiation culture medium for three days after inoculated for 25 days,and small bulbs in vitro,are studied.

以带鳞片叶的鳞茎盘为外植体，接种在添加激素的MS培养基上，通过激素组合筛选合适的培养基获得不定芽，同时对在预分化培养基上接种7D的外植体、接种25D后转接到分化培养基上3D的外植体、诱导产生的试管小鳞茎进行辐照诱变处理，研究了60COΓ射线对中国水仙丛生芽分化以及小鳞茎生长的影响。

In cider to breed new gern1asm for Narcissus tazetta, the bulb of Narcissus tazetta is used as explants. The adventitious buds is induced in MS media with hormone combination.γ-ray irradiation treatment of Narcissus tazetta combined with tissue culture is studied. Three materials, including the bulb basal plate tissues inoculated for 7 days, tissues transferred in fresh differentiation culture medium for three days after inoculated for 25 days, and small bulbs in tarn, are studied.

以带鳞片叶的鳞茎盘为外植体，接种在添加激素的MS培养基上，通过激素组合筛选合适的培养基获得不定芽，同时对在预分化培养基上接种7d的外植体、接种25d后转接到分化培养基上3d的外植体、诱导产生的试管小鳞茎进行辐照诱变处理，研究了60Coγ射线对中国水仙丛生芽分化以及小鳞茎生长的影响。

Bulb scale segments of Lilium longiflorum were used as original explants and shoot sections excised from shoot clusters arosed from the first explants as the secondary explants.

采用两步外植体法，即以百合鳞片叶为初始外植体，以从初始外植体上长出的芽为次级外植体，成功建立了麝香百合的高频离体再生系统。

The callus induction of licorices was easy. And the MS+1.0 mg/L NAA+1.0 mg/L 2, 4-D +1.0 mg/L 6-BA was the best medium to cotyledons, hypocotyls and radicles. And all the induction rates were 100%. 4. In the study of callus regeneration, only obtain the regeneration plants of hypocotyls callus,and its frequency was 4.57%.

甘草离体再生中愈伤组织的诱导较为容易，三种外植体子叶、下胚轴和胚根均以MS+1.0 mg/L NAA+1.0 mg/L 2，4-D+1.0 mg/L 6-BA为愈伤组织诱导的最佳培养基，在此培养基上，3种外植体愈伤组织诱导率均达到100%。

与外植体相关的网络解释 [注：此内容来源于网络，仅供参考]

Phytolacca americana：美洲商陆

[摘要]利用发根农杆菌菌株Ar1334与美洲商陆(Phytolacca americana)叶片外植体共培养转化体系,共获得58个发状根无性系(SL-1-58).以发根农杆菌Ri质粒TL-DNA上的rol C基因设计特异引物,对发状根进行PCR检测,得到了预期的560 bp目的片段,

encloser：外罩

外展过度hyperabductionsuperabduction | 外罩encloser | 外植体explant

escharotic：腐蚀剂, 苛性剂 <单词词性>[医]生焦痂性的

explant 移植 外植体 | escharotic 腐蚀剂, 苛性剂 [医]生焦痂性的 | intuitively 直觉地, 直观地

explant：外植体

8.外植体(explant)用于离体培养进行无性繁殖的各种植物材料. 9.脱分化(dedifferentiation) 植物已经分化的细胞在切割损伤或在适宜的培养基上诱导形成失去分化状态的、结构均一的愈伤组织或细胞团的过程.

explant：移植 <单词词性>外植体

blind drainage 内流水系 暗沟排水 | explant 移植 外植体 | escharotic 腐蚀剂, 苛性剂 [医]生焦痂性的

explant：外植体[用于植物材料];外植块[用于动物材料]

expansion factor 溶胀因子 | explant 外植体[用于植物材料];外植块[用于动物材料] | explantation 外植

explant culture：外植体培养

explant 外植体 | explant culture 外植体培养 | explantation 外植

explantation; tissue culture：组织培养

外植体(切下作培养的组织或器官的断片) explant | 组织培养 explantation; tissue culture | 爆发的演化 explosive evolution

scape：花葶

根据本课题多年来的初步研究发现,百合科多肉植物的花葶(Scape)可能比较适合作离体无性系的外植体[3,4],因此本项目以水晶掌花葶作外植体,试图通过较系统地愈伤组织诱导、植株再生等研究,为多肉植物引种和种质保存、快速繁殖与开发利提供依据.

explants：外植体,外植块

exogenousmltogen 外源促细胞分裂原 | explants 外植体,外植块 | explantation 外植培养