鉴定试验

Biuret test A test for the presence of proteins, in which sodium bydroxide is added to the test solution, followed by drops of 1% copper sulfate solution.

双缩脲试验：鉴定蛋白质存在的试验。在待检测溶液中加入氢氧化钠，然后滴加1％硫酸铜溶液。

Kit method and coagulase gene method, to authenticate staphylococci and evaluate coagulase test method. The strain will be test again if the result of 4 kind method are inconsistent.

和凝固酶基因法，鉴定葡萄球菌和评价凝固酶试验方法。4种方法结果不一致的菌株进行重复试验。

Then they were identified by serum plate agglutining test, HI test and serotype-specific monocolonal antibody. The results showed that 15 isolates among them were serotype A, and 4 isolates were serovar B. This results indicates that there are some new epidemiological characteristics of infectious coryza in china.

又将分离株用血清平板凝集试验、血凝抑制试验和型特异性单抗进行了血清型的鉴定，结果表明分离菌有15株为A型，4株为B型，说明我国鸡传染性鼻炎的流行已经出现了新的特点，在疾病预防和控制中应加以注意。

In order to understand the prevalence of conditions of the disease in Shandong Province, 6 strains of bacteria were isolated from 20 suspected Infectious Coryza chickens in vivo from five farms in Dezhou region of Shandong Province, and identified as Haemophilus paragallinarum by the bacterial culturing, biochemical tests sequencing of 16S rDNA gene, chicken embryo inoculation test.

为了了解该病在山东省的流行状况，从山东德州地区5个鸡场的20只疑似鸡传染性鼻炎鸡体内共分离到6株细菌，经细菌分离培养、生化试验、鸡胚接种试验和16s rDNA基因序列分析等方法鉴定为副鸡嗜血杆菌。

Tractor drawbar and noise test play an important role in theoretical study, performance analysis, quality appraisal of tractors.

拖拉机牵引和噪声试验是对拖拉机进行理论研究、性能分析以及对新产品作定型鉴定的最主要试验项目。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

Result］ It could be concluded through analyzing the model that both the mass uncertainties of quinoline phosphomolybdate precipitates and tested samples were from the resolving power and precision of balance and its maximum tolerance in assay certificate; the uncertainty brought in by volumetric flask was from the permissible error of 250 ml volumetric flask, the operation of increasing the volume to scale of volumetric flask while diluting solution and the difference between experimental temperature and the temperature at the time when the volumetric flask was calibrated; the uncertainty brought in by pipette was from the permissible error of itself, the operation of increasing the volume to intake scale while taking solution and the difference between experimental temperature and the temperature at the time when the pipette was calibrated.

结果］磷钼酸喹啉沉淀和试样质量的不确定度均来自天平的分辨力、精确度、鉴定证书上天平的最大允差，容量瓶引入的不确定度来自250 ml容量瓶本身的允许误差、稀释溶液时将体积增加到容量瓶刻度的操作和试验温度与容量瓶校准时温度的差异，吸量管引入的不确定度来自于吸量管本身的允许误差、吸取溶液时将体积增加到吸取刻度时的操作和试验温度与吸量管校准时温度的差异。

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

We made all-round serological tests to the two RhD-- individuals,including identification of blood group、Coombs test、antibody screening and identification、antibody titer and HDN test,together with investigation of genealogical blood group to all family members by classic hemagglutination methods.

我们采用经典的血细胞凝集法，对2个体进行了血型血清学检测，包括血型鉴定、抗人球蛋白试验、抗体筛选与鉴定、抗体效价检测与新生儿溶血病检测，并对两个家系所有家系成员进行家系血型调查。

In the experiment, the tea plant shoots were cultured in vitro, and inoculated artificially. The result showed the disease condition in vitro was basically consistent with that of the tea plantation in nature. Therefore, we thought this identification method of tea plant resistance to tea brown blight was feasible, especially a large number of cultivars.

本试验采用离体插枝培养法，人为造成伤口、人工接种并保湿对茶树品种进行抗性鉴定，室内人工接种与田间自然发病的情况基本一致，因此，可以认为此方法对茶树品种抗云纹叶枯病鉴定是可行的。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。