鉴定试验

Methods The samples were identified by FORTUNE.2000 of Fuxing Company and automatic animalcule identification system of Zhuhai Heima Bioengineering Co., Ltd. The drug sensitivity was tested by disk diffusion methods.

菌株鉴定采用复星公司FORTUNE.2000和珠海黑马生物工程有限公司全自动微生物鉴定系统，药敏试验用纸片扩散法。

Twenty varieties or materials of tomato with different resistance were used for experiment. Through testing the reaction condition and pH of extract of POD and PPO, identifying the genetic relationship of twenty varieties by POD isozyme and testing the biochemical indexes of resistance to ToMV, the main results were showed as follows:1. Classification of resistance: According to the campestral resistance to ToMV and the analysis experimental data ,the resistance of twenty varieties were divided into three kinds:z(1)resistant variety, including Zhong-za No9. Zao Hong-bao.

本试验以20个番茄品种或材料为试材，研究了过氧化物酶、多酚氧化酶酶液提取及反应适宜条件；应用POD同工酶对20个番茄材料进行亲缘关系的鉴定；对20份材料苗期室内接种ToMV后进行抗病性鉴定以及接种两周后所有材料POD、PPO、苯丙氨酸解氨酶的活性变化的研究，得出以下结论： 1。

This study was conducted to help identify gynoecium quickly and effectively. Our target was to develop a new method at the molecular level to overcome physical shortcomings.

快速、准确划分出黄瓜雌性系是蔬菜育种工作者面临的重大任务，为了弥补目前物理方法鉴定的缺陷，本试验开展分子水平上鉴定的研究。

Through morphological study, biophysical and biochemical tests ,we discovered that this strain was a kind of osmophilic yeast and can fermented glucose,sucrose and maltase.

分离筛选到了一株产赤藓糖醇能力高的菌种B84 5 ，通过对B84 5的形态、生理生化特性试验，发现该菌耐高渗，能发酵葡萄糖、蔗糖、麦芽糖，细胞为圆形、椭圆形，单边或多边芽殖，有真菌丝，在固体培养基上培养 7d后颜色开始变深，由黄色变成褐黑色，参阅真菌鉴定手册，初步鉴定为圆酵母属菌 Torulasp。

The thermal properties and mechanical properties were analyzed by Thermogravimetric analysis and Hardness Pencil Test and Universal Test Machine, respectively. The structure of prepared PI-MWNTs was characterized by Fourier transform infrared spectrometry. The morphology of PI-MWNTs were observed by scanning electron microscopy and transmission electron microscopy. The electrical conductivity was analyzed by Super Megohmmeter.

本研究中使用能量分散光谱仪来鉴定多壁奈米碳管纯化效果，热重损失分析仪、铅笔硬度计、万能试验机分别测定材料的热稳定性质和机械性质，傅立叶转换红外光谱仪来鉴定其化学结构，扫描式电子显微镜与穿透式电子显微镜来观察材料的剖面型态与奈米碳管和基材之间的分散性，超绝缘量测仪量测材料的电性。

According to breeding objective and the study on characteristics of wild southernwood resources, the plant types of southernwood were directionally screened by derived system method and group mixed method, the selected population were purified and their characters were identified, the variety comparative tests in muti-sites for many years were conducted for the purified types that passed identification.

方法］根据育种目标和对青蒿野生资源特征特性的研究，采用派生系统法和集团混合法对青蒿植株类型进行定向筛选，对中选群体进行纯化和性状鉴定，对通过鉴定的纯化类型进行多年多点品种比较试验。

Elegans. In the above experiments we also excluded defects in locomotion and thermotaxis test of 3 mutants coursed interfering on results. Next we tried to investigate the genetic pathway between tph-1, bas-1, daf-2 and foregone genes related to C.

之后将进一步确定所鉴定的基因与已知的影响线虫记忆行为的一些基因之间的遗传关系，发现试验中鉴定的基因与glr-1、dgk-3、eat-4之间存在不同程度的遗传关系，具体来讲，tph-1作为glr-1的上游调节子发挥作用，而位于dgk-3的下游。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

For the species identifying the bacterium parting for out, have been used for the BCG milk culture medium training an appraisal an experiment, the flavescence training 48 h queen bacterial colonies vicinity colour, may judge out that bacterium produces acid when growing kind; Have made the benzazole experiment, reaction result is feminine gender.

为了鉴定分离出的菌种，做了BCG牛乳培养基培养鉴定实验，培养48h后菌落周围颜色变黄，可判断出该菌种生长时产酸；还做了吲哚试验，反应结果为阴性。

Two organisms(SG1,SG2)were isolated from the affected birds,which were identified as Chlamydia psittaci by means of electromicroscopic observation,artificial culture media inoculation test,sulfanilamide sensitivity test and iodine staining.

从发病鸽分离到 2株菌(SG1、SG2 )，经电镜形态学观察、人工培养基接种试验、碘胺敏感性试验及碘染色试验鉴定为鹦鹉热衣原体。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。