鉴定试验

First, a chlorinator was self-designed in this paper, and dichloride of 2-methyl-1-propene was synthesized by 2-methyl-1-propene reacted with chlorine fast in this reactor. By means of the Orthogonal Experiments design and single factor experiments, the influence of several reaction variables on the yield were examined and the optimum reaction conditions under the intervallic operation were obtained, the yield and selectivity of dichloride of 2-methyl-1-prop-ene were more than 65% and 80% respectively. Second, the preparation of 2-methylene-1, 3-propanediol, which dichloride of 2-methyl-1-propene reacted with alkali was investigated. By means of the Orthogonal Experiments design and single factor experiments, the influence of several reaction variables on the yield were examined and the optimum reaction conditions under the intervallic operation were obtained, the conversion of dichloride of 2-methyl-1-propene was 100%. Moreover the preparation of MPO, which 2-methylene-1, 3-propanediol reacted with hydrogen, in presence of Al_2O_3-Pd catalyst, was gotten, and the product was appraised.

本文在氯化反应过程中，自行设计制作出了氯化管式反应器，在该反应器中异丁烯和氯气迅速混合反应生成二氯异丁烯，根据该氯化反应特征利用正交试验和单因素试验考察了有关因素的影响并优化得到了最佳工艺条件，二氯异丁烯的总收率达65％以上，选择性达80％；接着，对二氯异丁烯进行水解反应制得了2-甲烯-1，3-丙二醇，讨论了相关因素对水解反应的影响，并通过正交试验和单因素试验得到了较佳工艺条件，二氯异丁烯的转化率达100％；同时还对制备的2-甲烯-1，3-丙二醇在常压下采用三氧化二铝负载钯催化剂催化加氢得目的产物MPO，并对产品进行了鉴定，从而打通了整个工艺路线。

The size of bacteria,characteristic of stain,move about under light microscope and types of endospore were observed.

通过观察实验菌株的形态、染色特征、动力、芽孢的形态与位置，结合葡萄糖、阿拉伯糖、乳糖、肌醇发酵、卵磷脂试验、尿素水解试验、溶血性试验、青霉素抗性试验、溶菌酶抗性试验等，结果3种微生物被鉴定为蜡样芽孢杆菌、克柔念珠菌、枯草芽孢杆菌。

Selected the best cultivate condition by cultivated on four different media under different conditions; Observated morphology perperties; Studied on biology perperties by germ tube testing, Carbohydrate fermentation testing, Tween testing and Esculin dissociation; lysis testing.

选择四种不同的培养基分别在不同温度条件下培养，选出最优培养条件；通过光学显微镜、电子显微镜观察菌落及菌体的形态学特点；通过芽管试验、碳水化合物发酵试验、碳水化合物同化试验、沙堡培养基生长试验、过氧化氢酶试验、七叶苷分解试验、吐温试验研究该菌的生理、生化学特征；应用PCR技术通过特异性的引物扩增得到菌属特异性的片段，最后测定扩增片段的核苷酸序列，从而进行菌种的分析、鉴定。

The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段，应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段；在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法；检测方法的特异性试验结果表明，该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应；检测方法的敏感性试验结果表明，通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg；对20份临床样品检测中，有10份为AIV阳性，其中有5份为H5亚型病毒阳性。

In handling these problems, integrated test design and evaluation technology is studied in this thesis,the purpose of which is to reduce repetitiveness of test in the developing phase,guarantee quality of test data,make full use of various test i.

针对我国武器装备在其全寿命周期内经历的工作阶段多、各阶段技术状态各不相同但具有一定的继承性、而各阶段特别是全系统现场试验量较少的特点，分析了目前小子样试验方法存在的不足，提出了武器装备综合试验与鉴定的技术框架，重点介绍了支撑该技术框架的综合试验设计方法、小子样多状态试验数据融合处理方法以及战技指标综合评定方法的研究进展，指出了需要进一步研究和解决的问题

Results The Bartonella sp.strains could decompose disaccharide without disassociation of glycose,trisaccharide and spirits,and produce bacillosporin with positive of glucose oxidation and ureolysis test,negative of hydrogen sulfide production test and oxidase test....

结果检测到巴尔通体菌株具有分解双糖，不分解单糖、三糖和醇类，葡萄糖氧化阳性，硫化氢试验阴性、尿素分解试验阳性，氧化酶试验阴性，能产生多粘菌素等生化特性。结论全自动微生物鉴定系统能快速检测出巴尔通体一些特殊的生化反应特性。

Water stress is one of the most important abiological stress to maize seedling .In this study, we arm to identify the sensitive stage of maize seedling to water stress and select the material which tolerance to flooding. And we use waterlogging tolerance index to identify the sensitive stage of maize seedling. we have studied 20 inbred lines of maize which seedlings under water stress in three stage. The result show that the three leave stage is the most obvious stage to flood stress. In addition, we have analyzed ten of the inbred lines to re-act the waterlogging tolerance index. The result show good re-action. And the result also show that waterlogging tolerance index is a credible index to select waterlogging tolerance materials in seedling.

涝渍是玉米苗期主要的非生物逆境胁迫之一；本试验通过对20份玉米自交系进行苗期的淹水对照试验，采用了三个处理时期，并利用耐渍系数作为指标进行敏感时期的鉴定，结果表明三叶一心期是淹水的相对最佳时期；同时利用其中10份自交系对耐渍系数的重演性进行了分析，结果表明其重演性很好；同时结果还表明耐渍系数是耐渍性鉴定的一个可靠指标。

Considering the merits of in vivo studies in biotransformation i. e. activation or inactivation, protein-binding such as sex hormone binding protein , toxicokinetics, and more evaluative in hazard identification and risk assessment, we selected a battery of short-term and long-term in vivo studies, including uterotrophic assay in weaning mice and ovariectomized adult mice, an in vivo multiple endpoints assay, and a gestational and lactational exposure assay to identify the estrogenic effects of endosulfan on the offspring.

考虑到体内试验在代谢转化、血浆蛋白如性激素结合蛋白的结合、代谢动力学等方面比体外试验优越，在危害鉴定和危险度评定中更有价值，我们选择了多项体内试验，包括对幼年小鼠和卵巢切除成年小鼠的子宫增殖作用、对成年去势小鼠的体内多终点观察以及大鼠孕期和哺乳期接触对雄性子代生殖系统结构和功能的改变，即去雄性化或雌性化作用等，来验证硫丹的体内雌激素样作用。

From its the depart in splanchnic organization arrives bacterium of 2 individual plant, pass bacteriological method appraisal, one individual plant is deputy pig bloodsucking bacili, another individual plant is a pig streptococcic; will send check sample book have an inoculation to marc-145 cell, can observe cellular pathological changes, the pcr of virus fluid classics of results detects, for positive of blue ear ill viru

从其内脏组织中分离到2株细菌，通过细菌学方法鉴定，一株为副猪嗜血杆菌，另一株为猪链球菌；将送检样本接种到marc-145细胞，能观察到细胞病变，收获的病毒液经pcr检测，为蓝耳病病毒阳性；将送检的样本接种spf鸡胚，收获的鸡胚尿囊液有血凝性，血凝试验和血凝抑制试验鉴定为猪副黏病毒。

To update test technical system and build informatized test range, we discuss the establishment of informatized test technical system for naval military equipment, present an idea of multi-sensor information fusing based on network environment, and propose a concept for realizing the unidirectional transparentness of the test using multi-dimensional sensing systems, and establish a dynamic equation of the "quantized" equipment test system.

这一目标的提出，需要信息化试验技术体系的支撑。本文对创建海军军事装备信息化试验技术体系进行了探讨，论述了信息化试验技术体系的一些基本问题，并以知识处理为该技术体系的逻辑起点，提出了基于网络环境的多传感器信息融合思想，以及采用多维感知系统实现试验单向透明等概念，建立了装备试验系统&量子化&的动力学方程，旨在为建立装备信息化试验技术体系做有意义的探讨，这对提高信息化靶场的鉴定和试验能力具有现实意义。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。