鉴定

To better understand the roles of LsrA protein in nodulation, a series of analyses of nodules using scanning electron microscopy, genetic and biochemical approaches have been carried out. Our analyses suggest that the lsrA1 nodules contain bacteroids in the invasion and establishing zone only. The lsrA gene expression is active early in the invasion zone and activated later than bacA genes. The lost of LsrA protein dramatically reduced the expression of nifA and fixK, and completely blocked the expression of the nifH gene for nitrogenase. LsrA protein functions early in the bacteroid development and it is essential for the development nitrogen fixing bacteroids.

前期的工作中，苜蓿中华根瘤菌Rm1021中90个候选LysR基因已经被定向插入突变，并筛选在自生生长时期、共生生长时期的表型，以期寻找更多在自生状态或共生固氮中有功能的LysR转录因子。1 针对前期鉴定出的共生固氮必需的lsrA基因，我们应用了一系列扫描电子显微镜技术、生物化学、分子遗传学等方法，发现lsrA基因主要在根瘤侵染区开始表达，表达时序也在侵染阶段左右，但晚于bacA基因表达；LsrA蛋白缺失后根瘤固氮区中缺乏具有固氮能力的类菌体，nifA和fixK基因的转录水平降低，nifH基因的转录被完全阻断，因此LsrA蛋白为根瘤发育所必需，是新的根瘤发育信号传导途径成员。2 通过表型筛选我们鉴定了苜蓿中华根瘤菌的oxyR基因，并研究了它的调节特性。oxyR突变后，苜蓿中华根瘤菌对过氧化氢敏感性提高，适应性降低。

So it has very importance practical meanings to indentify the type of ball-pen ink with chromatography.

而圆珠笔种类的鉴定是很关键的一个步骤，为书写时间的鉴定奠定基础。

It's often required to exame the type and date of writing ink in the documents relate to criminal,economical or civil cases. So the identity of ball-pen ink is very importante for forensic science.

在法庭科学中，许多刑事、经济案件及民事纠纷中经常涉及到其种类鉴别和书写时间的鉴定，所以圆珠笔油墨的鉴定是倍受关注的。

The average interspecific divergence was 3.860%, and special sites were found in each chicken breed which was the bases to identifying chicken breeds. The DNA taxonomy of Bar1 used NJ was consistent with morphological taxonomy among the six indigenous chicken breeds. However, the analysis of Bar2 sequences of COⅠindicated that they were lower polymorphism and no special site in most chicken breeds which was not benefit to identify chicken breeds. The DNA taxonomy of Bar2 used NJ was not consistent with morphological taxonomy among the six indigenous chicken breeds.

本研究选择的2段序列中，以Bar1序列648bp（712位点到1359位点）多态性最高，突变位点最多，为24个，品种间平均多态性为3.860%，6个鸡种在此序列中有各自不同的特异位点，NJ聚类分析6个品种基本被聚为不同的类别，与形态学分类基本一致，该COⅠ基因的Bar1序列用于这些品种鉴定是可行的；而Bar2序列进行序列多态性分析，由于其多态性低，大多品种没有其特异位点，NJ聚类分析6个品种基本被聚为不同的类别，与形态学分类基本一致，不利于品种鉴定。

Feasibility study on application of DNA bar-coding technology in molecular identification of ScolytideaCO I gene of 15 bark beetle species collected from different regions and host is studied to discuss the feasibility of application of DNA bar-coding technology in Scolytidea molecular identification.

基于线粒体COⅠ基因的DNA条形码技术应用于小蠹科昆虫分子鉴定的可行性研究通过对采自不同地区、不同寄主的15个小蠹虫样本的线粒体COⅠ基因研究，对基于线粒体COⅠ基因的DNA条形码技术应用于小蠹科昆虫分子鉴定的可行性进行了探讨。

Further more,at present,there are tess systemic professional references about intercepted and captured bark beetle during quarantine in China,so it\'s time to establish a database of morphologic characteristics of bark beetle for quarantine in order to help quarantine inspection officer correctly identify them.Together with DNA bar-coding technique,the database will greatly promote the ability in identifying intercepted and captured bark beetle during quarantine.

此外，由于小蠹科昆虫种类繁多，目前对检疫截获小蠹虫的鉴定工作存在较大困难，且比较系统地反映我国进口货物中截获小蠹虫的专业资料较少，因此，急需建立针对我国进口木材中小蠹虫检疫用的形态图谱以加强检疫人员对其的识别；并通过分子生物学分类技术的研究，辅以DNA条形码技术以提高对其的鉴定水平。

At the same time, the identification of these two was designated as the national key laboratory gemstones, bear the state to entrust the task of identification.

同时这两家鉴定所被指定为国家重点宝石实验室，承担国家委托之鉴定任务。

MethodTo authenticate carefully the patients who have caught the nosocomial infection causing by the same bacteria within three weeks with the micro scan appraise systems of the France Bio Merieux API 20E and the USA Dade Behring. And test them by the K-B Medicine Susceptibility.

方法对集中在三周内发生同一种细菌引起的医院感染的病人采用法国梅里埃API 20E和美国德灵Micro Scan鉴定系统及K-B法药敏试验进行细菌的鉴定与药敏试验。

Methodto authenticate carefully the patients who have caught the nosocomial infection causing by the same bacteria within three weeks with the micro scan appraise systems of the france bio merieux api 20e and the usa dade behring.

方法对集中在三周内发生同一种细菌引起的医院感染的病人采用法国梅里埃api 20e和美国德灵micro scan鉴定系统及k-b法药敏试验进行细菌的鉴定与药敏试验。

To analyse the reason for one case of hemolytic transfusion reaction, antibodies in a patient's serum were identified using panel cells and Le phenotype cells, patient phenotype was identified by using anti-Le and anti-Le blood grouping reagents and the entire coding region of FUT3 gene was amplified by PCR and sequenced directly.

为了分析1例溶血性输血反应产生的原因，用谱细胞和Le表型细胞鉴定患者血清中的抗体，用抗Le和抗Le血型试剂鉴定患者红细胞表型，用PCR测序方法分析LE基因（FUT3基因）全编码区序列。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。