酵素

Cholesterol–cholesterol oxidase and glucose-glucose oxidase reactions were both coupled with different H2O2 production reactions to demonstrate the ability of biological luminescence and chromogen quantification and potential for diverse clinical diagnosis.

胆固醇–胆固醇氧化酵素和葡萄糖-葡萄糖氧化酵素氧化反应皆各自与不同过氧化氢光学反应结合，证明其具备冷光物质及发色物质定量能力，极具应用於不同临床分析的潜力。

Mutation of the gARID1 binding site in the cwp1 promoter resulted in a decrease of promoter activity and mRNA levels during vegetative growth and encystation, suggesting that the gARID1 binding sites are positive cis-acting elements of the cwp1 gene promoters in both trophozoites and encysting cells. Using cotransfection assay, we also found overexpession of gARID1 can increase luciferase activity and mRNA levels, while mutation of the gARID1 binding sites of the cwp1 promoter resulted loss of this transactivation.

我们也用萤光酵素基因接上cwp1基因启动子，如果将其cwp1启动子上的gARID1结合位置突变，也可以发现萤光酵素和讯息RNA的表现有明显的下；再用cotransfection assay，可发现gARID1会使萤光酵素和讯息RNA表现明显上升，然而如果将其cwp1启动子上的gARID1结合位置突变，这种转活化则消失。

In the second part of the thesis, we developed a very effective assay for the sulfotransferase that catalyzes the sulfation of serine and threonine. It utilized PAPS regenerated from 3-phosphoadenosine 5-phosphate by a recombinant PST using 4-methylumbelliferyl sulfate as the sulfuryl group donor. The change in the fluorescence intensity of 4-methylumbelliferone corresponded directly to the amount of active protein sulfotransferase.

第二部份则是探讨蛋白质亚硫酸转移酵素的活性检测方法，检测系统的主旨是用重组的PST催化受质4-methylumbelliferyl sulfate做为亚酸基提供者并转移至3'-phosphoadenosine 5'-phosphate再生PAPS ，此核苷酸的亚酸基再由蛋白质亚硫酸转移酵素催化，反应后所产生的4-methylumbelliferone之萤光变化可用来定量蛋白质亚硫酸转移酵素的活性。

Our main line is health enzyme products. Our product is made from pineapple, papaya, apple, kiwi fruits, etc. and high active yeast plant from california, U.S.A., so it contains high quality oligosaccharide. You may mix them with vegetable soup, yogurt or enzyme beverage as a dietary supplement. With years' experience in manufacturing and advanced technology, we assure you excellent qulity products at reasonable prices.

我们的酵素产品采用美国加州高活性酵母原菌，凤梨，木瓜，苹果，奇异果等新鲜水果制成，是优质寡糖；它可搭配精力汤，优酪乳及酵素饮料，并且可补充蔬果加热后流失之酵素，由於多年的制造经验及先进的制造技术，我们能提供品质优良且价钱合理的产品给我们的顾客。

The application of zymogen fertilizer or zymogen fertilizer with chemical fertilizer can both promote the development of water melon and raise the yield, between which the latter s effectiveness is better.

施用酵素菌肥或酵素菌肥配施化肥均能促进西瓜的生长发育，提高产量，以酵素菌肥配施化肥的处理效果最好。

Primary culture of osteoclasts cells were isolated from newborn Wistar rats. The effects of Drynaria fortunei on bone cell viability were determined by the method of MTT assay. The effects on osteoclasts cells activities were analyzed by acid phosphatase and lactate dehydrogenase.

本实验用Wistar新生老鼠做细胞初代培养，以体外试验模式探讨骨碎补对蚀骨细胞的影响，经由不同时间点的细胞存活率测试，分析酸性磷酸酵素和乳酸去氢酵素的含量来测量酵素活性。

At the depigmentation assay, DH and EH show dose-dependently inhibitory activities against mushroom tyrosinase, and the IC50 is 5.697 and 5.256 mM, respectively, and show noncompetitively inhibitory modes in the present of tyrosine, but not DOPA. The control synthetic products, D and E, have no inhibitory effects on tyrosinase. Using tyrosinase activity staining in PAGE gels, both DH and EH show dose-dependently inhibitory pattern in the presence of tyrosine, while, EH also could suppress tyrosinase activity in the presence of DOPA.

而美白活性方面，以商品酪胺酸以商品酪胺酸酵素进行实验，当基质为酪胺酸时，发现DH与EH具浓度相关抑制效果，IC50分别为5.697与5.256 mM，且其相对於基质酪胺酸之抑制型态皆为非竞争型抑制，而其结构类似物之对照化合物D与E则不具此活性；但当基质为多巴时则皆不具此活性；利用原态PAGE电泳活性染色亦发现抑制酪胺酸酵素结果，但EH在基质为多巴时也能下降酪胺酸酵素活性。

Inflorescence development was divided into four stages, the initiating expansion pseudobulb, initiating inflorescence (1-3 cm in flower stalk length), the initial-branching flower stalk, and full bloom inflorescence, respectively. The activity variations of mannan cleaving enzymes,-mannosidase (EC 3.2.1.25), endo--mannanase (EC 3.2.1.78), and the mannan synthesizing enzyme, GDP-mannose pyrophosphorylase (EC 2.7.7.13), were analyzed during different stages to better understand the role of mannan-metabolizing enzymes during inflorescence development of the Oncidium.

将花梗发育过程分为四个阶段，分别为假球茎刚膨大、刚抽梗（花梗长度约1-3公分）、花梗刚分叉以及花盛开，分析参与分解甘露聚醣之酵素－β-mannosidase（EC 3.2.1.25）及endo-β-mannanase（EC 3.2.1.78），与合成甘露聚醣之酵素－GDP-mannose pyrophosphorylase（EC 2.7.7.13），在不同阶段假球茎内的活性变化，了解在花梗发育过程中代谢甘露聚醣之相关酵素所扮演的角色。

The activities of superoxide dismut ase and glutathione peroxidase were elevated with increased dur ation of the supplementation. However, no differences between the LA and HA g roups were observed.

抗氧化酵素系统中的超氧化歧化酵素(superoxide dismutas e；SOD)和麸胱甘太过氧化酵素(glutathione peroxidase；GSH-Px)的活性均随著抗氧化营养素补充时间的增长而显著上升，但高剂量组和低剂量组的上升程度相似。

For long-term treatments, the rat administered daily by gavages for six weeks, the results of OGTT showed that guava fruit water or alcohol extracts could reduce the plasma glucose level in the diabetic Wistar rats, however there was on significant difference in insulin level among all tested groups. The results of carbohydrate metabolism showed diabetic Wistar rats administered with 200 or 400 mg/kg BW of guava fruit water or alcohol extracts could decrease more than 15% glucose-6-phosphatase and fructose-1,6-bisphosphatase activities, and it also increase more than 12% glucose-6-phosphate dehydrogenase activities to reduce inhibitory hexokinase from reducing glucose-6-phosphate level.

在醣类代谢方面，实验结果发现糖尿病鼠灌食番石榴果实水及乙醇萃取物六周后，肝脏醣质新生作用中丙酮酸合成肝醣之关键酵素glucose-6-phosphatase及fructose-1，6-bisphosphatase的酵素活性会降低15%以上；另一方面肝脏glucose-6-phosphate dehydrogenase酵素活性则明显增加12%以上，并造成glucose-6-phosphate浓度减少，进而降低其对hexokinase的抑制(因为hexokinase受其代谢产物G-6-P的浓抑制)，因此加速肝脏葡萄糖的糖解作用。

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如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。