转数

Northern blot analysis showed that the GFP gene was correctly expressed in the transgenic plants. Green fluorescence was observed in roots and immature embryos of transgenic plants by fluorescence microscopy.Progeny analysis showed that GFP insertions were inherited in T_2 generation with normal expression.Both Mendelian and non-Mendelian inheritances were observed in T_1 progenies of transgnic maize,and non-Mendelian inheritances were observed in T_2 progenies.

Southern blot结果表明GFP基因以低拷贝数、简单的方式(1～3条Southern杂交带)整合到玉米基因组；Northern blot结果表明GFP基因在转录水平上表达；在转基因植株的根和幼胚中观察到GFP表达；转基因植株的后代遗传分析表明GFP基因能够遗传至T_2代并正常表达，部分转基因株系中的GFP基因以孟德尔遗传方式传递给T_1代，但T_2代不符合孟德尔分离比。

By using electroporation transfects,the TGF〓expression vector were introduced into HL-60 cells.Thecells were allowed to grow for 1 day before beingsubjected to selection for the ability to grow in mediumcontaining 600ug/ml geneticin.Stable cell clones resistantto G418 sulfate were obtained after 2-3 WK growth withdrug-containing medium.Southern blot hybridization wascarried out.RNA slot have higher expression of TGF〓mRNA in HL-60 transfected.All results followed suggestedthat TGF〓 have suppressed the maliganant phenotype of HL-60 cells.

利用电击法将〓表达载体导入HL-60细胞中，经G418筛选培养20天后再行Southern杂交检测外源〓整合情况，利用RNA狭线杂交法证明了在转录水平上表达有所提高，进一步进行了流式细胞仪测定DNA含量，使细胞在〓期产生生长阻滞作用，细胞生长速度，在软琼脂中成集落数、在组织中的浸润性及在裸鼠中的成瘤性均发生变化，我们的实验结果表明TGF〓对HL-60细胞具有抑制恶性表型的作用。

The Shanghai number permanent RJ mold has made the limited companyto be established since 1996, established Wicks the brandseries mold, passes through more than 10 years development, hasestablished the leading status in the national mold profession,unceasingly grows along with the company, we already become from thenumerical control knee bend mold to the numerical control ZTCM and the plate cutter bit omni-directional numerical controlplate work processing equipment cutting tool mold supplier.

上海数恒刃具模具制造有限公司自1996年成立以来，创立了威克斯品牌系列模具，经过十多年的发展，已在全国模具行业确立了领先地位，随着公司不断成长，我们已经成为从数控折弯模具到数控转塔冲模及剪板机刀片的全方位数控钣金加工设备刀具模具的供应商。

The Shanghai number permanent RJ mold has made the limited companyto be established since 1996, established Wicks the brandseries mold, passes through more than 10 years development, hasestablished the leading status in the national mold profession,unceasingly grows along with the company, we already become from thenumerical control knee bend mold to the numerical control ZTCM ＆ the plate cutter bit omni-directional numerical controlplate work processing equipment cutting tool mold supplier.

上海数恒刃具模具制造有限公司自1996年成立以来，创立了威克斯品牌系列模具，经过十多年的发展，已在全国模具行业确立了领先地位，随着公司不断成长，我们已经成为从数控折弯模具到数控转塔冲模及剪板机刀片的全方位数控钣金加工设备刀具模具的供应商。

The Shanghai number permanent RJ mold has made the limited companyto be established since 1996, established Wicks the brandseries mold, passes through more than 10 years development, hasestablished the leading status in the national mold profession,unceasingly grows along with the company, we already become from thenumerical control knee bend mold to the numerical control ZTCM ＆ the plate cutter bit omni-directional numerical controlplate work processing equipment cutting tool mold supplier.

上海急招大中专毕业生，月薪5000，企业订单培养100%推荐就业——赞助商链接上海数恒刃具模具制造有限公司自1996年成立以来，创立了威克斯品牌系列模具，经过十多年的发展，已在全国模具行业确立了领先地位，随着公司不断成长，我们已经成为从数控折弯模具到数控转塔冲模及剪板机刀片的全方位数控钣金加工设备刀具模具的供应商。

The parade stretched for several kilometers, and when it turned right from Chungshan South Road into Ketegelan Boulevard, it was blocked in front of Kungyuan Road by steel wire barricades and a military police cordon.

绵延数公里的行列，从中山南路右转凯达格兰大道时，受阻於公园路前的铁丝蛇笼和宪警封锁线。

Introduces the raw cotton property effect on yarn strength ,and deals throug h the experiment with the relationship between cotton yarn strength and cotton f iber thinness,the rate of short-staple and its number of natural convolution,so as to provide theoretical reference when using cotton fiber reasonably.

介绍原棉性能对纱线强力的影响，并通过实验探讨棉纱强力与棉纤维线密度、短绒率、天然转曲数之间的关系，为合理使用棉纤维提供了理论依据。

Results show that the performance of this VCRT basically reaches the original objective,which indicates that a convergent-divergent blade passage in the high pressure rotor and a convergent-divergent meridian plane of the VCRT are suitable for the VCRT.

结果表明，对转涡轮各方面性能基本达到了设计目标，说明高压动叶采用收扩型叶栅通道且整个涡轮子午流道也采用收扩结构的设计可以获得满意性能；三排叶片仍有改进余地，即涡轮效率还有进一步提升的潜力；高压动叶出口沿整个叶高均为超声速，出口马赫数节距平均值在1.4~1.6之间；高压动叶尾缘斜激波强度以中展为中心向两侧递减；涡轮性能随出口背压的增加而下降；涡轮出口背压的变化对高压转子性能基本没有影响，但对低压转子性能的影响较大。

Businesses are losing millions of dollars, and products are more expensive due to white-collar crime.

由于白领犯罪，企业界每年损失数百万美元，产品也因之更贵。转帖于 Xue163.com_英语词汇

By establishing functional relations between concurrence and the generalized partial transpositions, we derive analytical lower bounds of concurrence for multipartite systems.

通过这个关系，我们将判定多体量子态的纠缠问题转化为计算推广的部分转置的迹范数是否大于1的问题。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。