胚胎的

The development of embryos from diploid somatic cells in embryo culture.

是胚胎培养过程中，二倍体细胞发育为胚胎的过程。

Expression was predominantly in extraembryonic tissues of E7.5 mouse embryo.

ZNF18主要在E7.5小鼠胚胎的胚外组织表达，E8.5出现了胚胎躯干前端表达。

Sheep embryo with high-purity,high-quality and high security features to extract the cells from the completion of the injection can be divided into three phases:-Using the most advanced chemical and biological equipment from sheep embryos to extract the liver activity of the protein and remove impurities,heat-sensitive eggs White matter and may result in allergic reactions of various types of risk factors-The adoption of advanced technology to extract the cells separated,thus the effectiveness of different cells,such as chest Gland cells,placental cells,liver cells and so on dozens of different types of-Using the most advanced technology to maintain the freeze-dried cells of live sheep-embryo for the first time the use of only afew hours to keep The cell activity,and now,with the continuous development of science and technology,after the extract ion of the cells have been able to keep to five years.

羊胚胎素具有高纯度、高品质和高安全性的特点，从细胞的提取到针剂的完成可分为三个阶段：-运用最先进的生化磁粉探伤仪，从小羊胚胎的肝脏中提取活性蛋白质，并去除杂质、热敏感蛋白质及可能导致过敏反应的各类危险因子-通过先进的技术对提取的活性细胞进行分离，从而得到具有不同功效的活性细胞，如胸腺细胞、胎盘细胞、肝脏细胞等几十种不同类型-采用目前最先进的冻干技术来保持细胞鲜活性，羊胚胎素在最初使用时只能保持几小时的细胞活性，而如今，随着科学技术的不断发展，提取后的细胞活性已经可以保持到5年。

From April to September, several clusters of bamboos, 3 ~ 4 m long, 1 ~ 2 m wide, were set on the sea bed at a depth of 16 to 20 m to attract their spawning, and the egg-strings were then transported to the laboratory. When the development of the embryo reached stage 24, iris of eyes being prominent as a colour circle and statolith being formed, they were transferred into different rearing conditions, i.e., 20, 25, 30, 35 o/oo and 15, 20, 25, 30 oC. The durations from stage 24 to hatching were different among all different rearing conditions. The statoliths were extracted and mounted in Crystal Bond thermoplastic cement for reading their growth rings.

每年的四月到九月为东北角软丝主要的产卵季节，竹丛被设置在沙质海床上吸引成熟的个体前来产卵，将采集到的卵串带回实验室中培养，当胚胎发育至第二十四期的时候(胚胎的虹膜及平衡石开始生成)，这些胚胎分别被转换至不同的温度及盐度条件之下培养，温度被控制在15， 20， 25， 30 oC 四种条件下，盐度则分为20， 25， 30， 35 o/oo 四种条件，共组成十六种不同的培养条件，从平衡石生成至幼体孵出所需要的时间随著培养条件的不同而有所差异，孵化所需要的时间会随著培养的温度而有很大的变化，低温的条件会将所需的时间增长许多，在正常的条件下(25oC，35o/oo)，从平衡石出限制肤出约需要9~16天。

Many fetal liver cells were EGFP positive in E13. 5 embryos as well as most of yolk sac was EGFP positive.

在胚胎发育到13.5天时，有大量肝脏组织细胞表达EGFP，其包围胚胎的胎膜上仍有大量细胞表达EGFP。

In conclusion, either electrofusion parameter or developmental stage of donor embryos have a significant effect on the efficiency of NT, NT embryos require different concentration of serum at their different development stages.

结果表明，电融合参数和供体胚胎的发育阶段以及受体卵母细胞的状态对NT效果有显著影响，在NT胚胎的不同发育阶段采用不同的血清种类和浓度可提高其胚胎发育能力。

During the process of nuclear reprogramming, the zygotes of the normal embryos and the donor nuclei of thecloned embryos cease their unique repertoire of gene expression and reset their gene expression to the totipo-tent status, and then the normal and cloned embryos redifferentiate from the totipotent status to variousdifferentiated states for tissue generation or organogenesis.

通过细胞核重编程，首先，受精卵和克隆胚胎的供体核停止其特有的基因表达程序，恢复为全能状态的基因表达程序；然后，受精胚胎和克隆胚胎的细胞再从全能状态重新进入分化状态，最终形成各种组织和器官。

Experiment Ⅴ: Mouse polyploid embryos were produced by electrofusing 2-cell blastomeres successionally.

实验六、通过2-细胞卵裂球交换制作2n／4n嵌合小鼠胚胎，对这种胚胎的发育能力进行了评价。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分为4个部分，第一部分对影响猪卵母细胞体外成熟和孤雌激活后胚胎分裂的几个重要条件进行了比较研究，确立了一种较好的培养方法：与颗粒细胞共培养，找到了一种适合猪卵母细胞体外成熟的培养基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸，成熟率和分裂率分别为(86.7±3.35)%和(86.3±4.16)%，国外报道的最高成熟率为(85.7±4.1)%；第二部分对猪卵母细胞孤雌激活的化学方法进行了研究，确立了化学激活猪卵母细胞的两种最佳方法：1将成熟的去卵丘颗粒细胞的MII期卵母细胞用20μmol/Lionomycin作用30min，再将卵母细胞培养于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培养液中，卵裂率和桑囊胚发育率达到(76.7±7.6)%和(37.1±6.4)%2将成熟的去卵丘颗粒细胞的MII期卵母细胞在200μM/L的Thimerosal中处理20min，再与8mM的DTT共孵育30min，卵裂率和桑/囊胚形成率为(81.0±2.8)%和(39.6±2.7)%；第三部分对孤雌激活胚胎的培养条件进行了研究，确立了一种最佳的胚胎培养条件：在SOF简单培养基中添加颗粒细胞进行前3天的培养，然后转入添加胎牛血清的NCSU—23培养基并和输卵管上皮细胞进行后期的培养，其桑椹胚和囊胚的发育率为(59.5±3.2)%；第四部分研究了IGF-I

Embryos were explanted and cultured 4 hours after CP administration. On d 10.5, culture was terminated and embryos were examined for yolk sac diameter, head length and crown-rump length and teratogenic effects of CP.

本文应用小鼠全胚胎培养技术，通过妊娠d 8分别ip 5，10，15和20 mg.kg-1环磷酰胺，研究了该药对器官原基形成期胚胎的致畸作用，并对其致畸机理作了初步探索。

With the development of highway construction, the speed of cars has been gradually increased.

随着公路的发展，汽车的车速也在不断提高。

So we basically had to develop a bond with the animal in terms of socializing with it.

所以我们就主要想通过跟它沟通的方式来建立一种良好的关系。